Nanodispenser for attoliter volume deposition using atomic force microscopy probes modified by focused-ion-beam milling

Meister, André; Liley, Martha; Brugger, Jürgen; Pugin, Raphaël; Heinzelmann, H.

doi:10.1063/1.1842352

Cited by 110 publications

(96 citation statements)

References 11 publications

(6 reference statements)

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“…More recently, such contact methods have been extended to spotting of DNA microarrays 15,16 and the utilization of AFM tips for transfer of volumes in the attoliter range. [17][18][19][20] From the perspective of microfluidic integration, it would be ideal if all fluidic processes, such as dispensing, routing, mixing, activation and quenching of chemical reactions, could be implemented by utilizing a single tunable external mechanism. It has been demonstrated, for example, that ionic or electrolytic solutions can be mobilized and dispensed along a surface by the technique known as electrowetting.…”

Section: Introductionmentioning

confidence: 99%

Planar digital nanoliter dispensing system based on thermocapillary actuation

Darhuber

Valentino²,

Troian

2010

Lab Chip

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We provide guidelines for the design and operation of a planar digital nanodispensing system based on thermocapillary actuation. Thin metallic microheaters embedded within a chemically patterned glass substrate are electronically activated to generate and control 2D surface temperature distributions which either arrest or trigger liquid flow and droplet formation on demand. This flow control is a consequence of the variation of a liquid's surface tension with temperature, which is used to draw liquid toward cooler regions of the supporting substrate. A liquid sample consisting of several microliters is placed on a flat rectangular supply cell defined by chemical patterning. Thermocapillary switches are then activated to extract a slender fluid filament from the cell and to divide the filament into an array of droplets whose position and volume are digitally controlled. Experimental results for the power required to extract a filament and to divide it into two or more droplets as a function of geometric and operating parameters are in excellent agreement with hydrodynamic simulations. The capability to dispense ultralow volumes onto a 2D substrate extends the functionality of microfluidic devices based on thermocapillary actuation previously shown effective in routing and mixing nanoliter liquid samples on glass or silicon substrates.

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Section: Introductionmentioning

confidence: 99%

Planar digital nanoliter dispensing system based on thermocapillary actuation

Darhuber

Valentino²,

Troian

2010

Lab Chip

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“…The cantilevers have been produced using standard microfabrication technique, whereas, the nanoaperture, shown in Fig. 3, has been fabricated using FIB technique [10]. Square and circular apertures with dimensions down to 80 nm have been drilled into the tip apex of the hollow cantilever, which nicely illustrates that FIB technique is, in contrast to standard fabrication methods, not limited to flat surfaces.…”

Section: Introductionmentioning

confidence: 99%

Focused Ion Beam: A Versatile Technique for the Fabrication of Nano-Devices

Santschi¹,

Przybylska

Guillaumée

et al. 2009

Practical Metallography

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“…A set of technologies are available for producing such nanoarrays, e.g. atomic force microscopy (AFM) [25], dip-pen nanolithography (DPN) [26], nanofountain probe [27,28], nanoimprint lithography [16], and various nanodispensers [20,22,29]. Albeit successful, these nanoarray layouts have been found to be associated with a set of key methodological shortcomings.…”

Section: Introductionmentioning

confidence: 99%

Generation of miniaturized planar ecombinant antibody arrays using a microcantilever-based printer

et al. 2014

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Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm−2) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, BioplumeTM—consisting of an array of micromachined silicon cantilevers with integrated microfluidic channels—to produce miniaturized, multiplexed, and high-density planar recombinant antibody arrays for protein expression profiling which targets crude, directly labelled serum. The results demonstrated that 16-plex recombinant antibody arrays could be produced—based on miniaturized spot features (78.5 um2, Ø 10 μm) at a 7–125-times increased spot density (250 000 spots cm−2), interfaced with a fluorescent-based read-out. This prototype platform was found to display adequate reproducibility (spot-to-spot) and an assay sensitivity in the pM range. The feasibility of the array platform for serum protein profiling was outlined.

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Nanodispenser for attoliter volume deposition using atomic force microscopy probes modified by focused-ion-beam milling

Cited by 110 publications

References 11 publications

Planar digital nanoliter dispensing system based on thermocapillary actuation

Planar digital nanoliter dispensing system based on thermocapillary actuation

Focused Ion Beam: A Versatile Technique for the Fabrication of Nano-Devices

Generation of miniaturized planar ecombinant antibody arrays using a microcantilever-based printer

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