Nanocrystalline calcium sulfate/hydroxyapatite biphasic compound as a TGF-β1/VEGF reservoir for vital pulp therapy

Chiang, Yu‐Chih; Chang, Hao-Hueng; Wong, Ching-Ching; Wang, Yiping; Wang, Yin‐Lin; Huang, Wen-Hsuan; Lin, Chang‐Yu

doi:10.1016/j.dental.2016.06.013

Cited by 33 publications

(25 citation statements)

References 42 publications

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“…Sequences of highly purified primers are outlined in Table 1. Briefly, cells were plated on 6 well plates at the density of 2×10 5 and incubated in medium with different extracts mediums for 10 days. Then the total RNA was isolated by using trizol (Takara Bio, Shiga, Japan) and synthesized complementary DNA using the RT Reagent Kit (Takara Bio) according to the manufacturer's instruction.…”

Section: Real-time Quantitative Polymerase Chain Reaction (Realtime Pcr)mentioning

confidence: 99%

“…To assess the formation of calcium nodule, alizarin red S staining assay was carried out. HDPCs were seeded into 6 well plates at the density of 2×10 5 For Western blot analysis, 30 μg of total protein was separated on 12% sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Pierce Biotechnology, Rockford, IL, USA) and then transferred to a polyvinylidene fluoride membrane. The membranes were blocked with 5% skim milk for 1 h. They were then rinsed and incubated overnight with antibodies against DSPP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and β-actin (Cell Signaling Technology) with 1:2000 dilution at 4°C.…”

Section: In Vitro Mineralization Assaymentioning

confidence: 99%

“…Pulp capping biomaterials are adopted to stimulate the pulp dentinogenic response in VPT, which are required to possess excellent physicochemical properties, biocompatibility, and more importantly, the ability to promote the differentiation of dental pulp cells and dentin formation 5) . First introduced to the clinical dentistry in 1921, Calcium hydroxide (CH) has been taken as the gold standard for pulp capping materials, which shows excellent ability in inducing dentin regeneration and antimicrobial characteristics.…”

Section: Introductionmentioning

confidence: 99%

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Effects of silicate-based composite material on the proliferation and mineralization behaviors of human dental pulp cells: An <i>in vitro</i> assessment

Tang

et al. 2018

Dental Materials Journal

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The objective of this study was to investigate the effects of a silicate-based composite material on proliferation and mineralization of human dental pulp cells (hDPCs), which was compared with those of calcium hydroxide (Ca(OH), CH) and tricalcium silicate (CaSiO, C3S). HDPCs were cultured with CH, C3S and tricalcium silicate/dicalcium silicate (CaSiO/CaSiO, C3S/C2S) composites extract. The CCK-8 assay showed that the composite material stimulated the proliferation of hDPCs. The odontogenic marker genes and DSPP protein expression were more significantly up-regulated by the C3S/C2S composite material compared with pure CH and C3S. HDPCs cultured with composite material extract exert stronger ALP activity and alizarin red S staining. C3S/C2S composite material was advantageous over pure C3S by showing enhanced ability to stimulate the proliferation and odontogenic differentiation of hDPCs, suggesting that the C3S/C2S composite materials possess desirable biocompatibility and bioactivity, and might be a new type of pulp-capping agent and dentin alternative materials.

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Section: Real-time Quantitative Polymerase Chain Reaction (Realtime Pcr)mentioning

confidence: 99%

Section: In Vitro Mineralization Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of silicate-based composite material on the proliferation and mineralization behaviors of human dental pulp cells: An <i>in vitro</i> assessment

Tang

et al. 2018

Dental Materials Journal

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“…The main substances utilized for impression taking are compound and alginate. 17,18 Alginate is mainly utilized when removable appliances are placed. 17 Compound, on the other hand, is used with fixed appliances because of its high stability and accuracy.…”

Section: Introductionmentioning

confidence: 99%

“…17 Compound, on the other hand, is used with fixed appliances because of its high stability and accuracy. 18 Taking impression may be carried out with or without impression trays. Impressions are usually used for bilateral rather than unilateral fixed space maintainers application.…”

Section: Introductionmentioning

confidence: 99%

Space maintainers application, indication and complications

Albati

Showlag

Akili

et al. 2018

Int J Community Med Public Health

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Dental space maintenance is essential in cases of premature loss of primary dentition to prevent malposition, supra-eruption, impaction, or crowding of the developing permanent teeth. Many types of space maintainers are available and each type is specifically designated for particular indications. Space maintainers have many classifications according to their design, their fixation pattern, their position, and their site. They can be fixed, semi-fixed, or removable, with or without loops, wires, and/or bands, unilateral or bilateral, and are placed on mandibular or maxillary arches. The most commonly used space maintainers are loops and bands, fixed lingual arches, Nance appliances, and transpalatal arches. Though space maintainers are generally safe, several complications are sometimes inevitable. This article will review the different types of space maintainers and their application and will discuss their indications and potential complications.

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