Effects of silicate-based composite material on the proliferation and mineralization behaviors of human dental pulp cells: An &lt;i&gt;in vitro&lt;/i&gt; assessment

Tang, Wu; Xu, Chunxiang; Du, Ruxu; Wen, Yumei; Chang, Jiang; Huan, Zhiguang; Zhu, Yun

doi:10.4012/dmj.2017-328

Cited by 11 publications

(9 citation statements)

References 45 publications

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“…In addition, Shie et al 47) found that compared to silicates with a lower silicon content, silicates with a higher silicon content could promote cell adhesion, proliferation and differentiation to osteoblasts, which suggests that it is important to pay attention to the silicon content when designing pulp-capping agents. In our previous study 48) , a silicate-based composite material with Si/Ca molar ratios of 1:3 and 1:2 also promoted mineralization compared to CH.…”

Section: Discussionmentioning

confidence: 79%

Silicate bioceramics elicit proliferation and odonto-genic differentiation of human dental pulp cells

Peng

Huan

et al. 2022

Dent. Mater. J.

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This study aimed to investigate the effects of silicates on the proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. HDPCs were cultured in the presence of calcium silicate (CS) extracts, while calcium hydroxide (CH) extracts and culture medium without CH or CS were used as the control groups. The calcium and phosphorus ion concentrations in the CS were similar to those in the control groups, but the concentration of silicon ions in the CS extracts was higher than that in the control groups. HDPCs cultured with CS and CH extracts at dilution of 1/128 proliferated significantly more than those cultured with the control treatments. CS extracts promoted cell migration, enhanced the expression of odontogenic marker genes and conspicuously increased odontogenesis-related protein production and the release of cytokines, suggesting that CS bioactive ceramics possess excellent biocompatibility and bioactivity and have the potential for application as pulp-capping agents.

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Section: Discussionmentioning

confidence: 79%

Silicate bioceramics elicit proliferation and odonto-genic differentiation of human dental pulp cells

Peng

Huan

et al. 2022

Dent. Mater. J.

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“…Although this seems to be counterintuitive, it is possible that the high alkalinity of the calcium silicate‐based sealer media can up‐regulate alkaline phosphatase activity and enhance mineralization (Wu et al . 2018). Moreover, the main component of both calcium silicate‐based sealers is calcium silicates, whose mineralization capacity has previously been reported (Zordan‐Bronzel et al .…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and bioactivity potential of the new Endosequence BC Sealer formulation HiFlow

et al. 2020

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AimTo evaluate in a laboratory setting the effects of Endosequence BC Sealer HiFlow (Brasseler USA, Savannah, GA, USA), a novel calcium silicate‐based sealer developed for use in warm canal filling techniques, on human periodontal ligament stem cells (hPDLSCs).MethodologyEluates of EndoSequence BC Sealer HiFlow (BCHiF) (Brasseler USA), EndoSequence BC Sealer (BCS) (Brasseler USA) and AH Plus (AHP) (Dentsply DeTrey GmbH, Konstanz, Germany) were placed in contact with hPDLSCs. The characterization of the chemical elements of the root canal sealers was assessed using scanning electron microscopy and energy‐dispersive X‐ray analysis (SEM‐EDX). Inductively coupled plasma‐mass spectrometry (ICP‐MS) was used to determine the ion release of the sealers. MTT assay and wound healing techniques were used to determine cell viability and migration, respectively. Cell morphology and cell attachment were assessed using a direct contact technique of hPDLSCs onto the surface of the sealers and analysed by SEM. The bioactivity potential was carried out with the Alizarin Red and qPCR testing methods. The statistical differences were evaluated using one‐way anova and Tukey’s test (P < 0.05).ResultsICP‐MS and EDX revealed significantly more zirconium in BCHiF than BCS (P < 0.05), whereas BCS had slightly higher levels of Ca2+ than BCHiF (P < 0.05). The cell viability assay revealed no relevant differences between BCS and BCHiF when compared with the control group (P > 0.05). Both BCS and BCHiF had similar rates of cell migration to the control group at 24 and 48 h. Cell morphology and adhesion capacity were also similar for BCS and BCHiF groups, whilst the AHP group was associated with reduced adhesion capacity. The Alizarin Red assay revealed a significant difference between the BCS and the control group (P < 0.001), as well as for the BCHiF group (P < 0.001). Finally, BCS and BCHiF promoted overexpression of osteo/cementogenic genes.ConclusionsIn general, EndoSequence BC Sealer HiFlow possesses suitable biological properties to be safely used as a root canal filling material and promote increased expression of oste/cementogenic genes by hPDLSCs.

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“…We chose this cell type because, in the indications of these materials, PDLSCs have contact with the periodontal ligament which are at the root-end cavity when periapical surgery is done or when iatrogenic perforation is repaired it is next to the periodontal ligament [10,21]. Other studies have used dental pulp stem cells with direct pulp capping materials [22], or osteoblast cells when they have also studied endodontic cements [23,24]. Therefore, the clinical situation is crucial for the choice of cell line.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

et al. 2020

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New bioactive materials have been developed for retrograde root filling. These materials come into contact with vital tissues and facilitate biomineralization and apical repair. The objective of this study was to evaluate the cytocompatibility and bioactivity of two bioactive cements, Bio-C Repair (Angelus, Londrina, Pr, Brazil) and TotalFill BC RRM putty (FGK, Dentaire SA, La-Chaux-de-fonds, Switzerland). The biological properties in human periodontal ligament stem cells (hPDLSCs) that were exposed to Bio-C Repair and TotalFill BC RRM putty were studied. Cell viability, migration, and cell adhesion were analyzed. Moreover, qPCR and mineralization assay were performed to evaluate the bioactivity potential of these cements. The results were statistically analyzed using ANOVA and the Tukey test (p < 0.05). It was observed that cell viability and cell migration in Bio-C Repair and TotalFill BC RRM putty were similar to the control without statistically significant differences, except at 72 h when TotalFill BC RRM putty was slightly lower (p < 0.05). Excellent cell adhesion and morphology were observed with both Bio-C Repair and TotalFill BC RRM putty. Both cements promoted the osteo- and cementogenic differentiation of hPDLSCs. These results suggest that Bio-C Repair and TotalFill BC RRM putty are biologically appropriate materials to be used as retrograde obturation material.

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Effects of silicate-based composite material on the proliferation and mineralization behaviors of human dental pulp cells: An <i>in vitro</i> assessment

Cited by 11 publications

References 45 publications

Silicate bioceramics elicit proliferation and odonto-genic differentiation of human dental pulp cells

Silicate bioceramics elicit proliferation and odonto-genic differentiation of human dental pulp cells

Chemical composition and bioactivity potential of the new Endosequence BC Sealer formulation HiFlow

In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

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