Nanocomposite Porous Microcarriers Based on Strontium-Substituted HA-<i>g</i>-Poly(γ-benzyl-<scp>l</scp>-glutamate) for Bone Tissue Engineering

Yan, Shifeng; Xia, Pengfei; Xu, Song; Zhang, Kunxi; Li, Guifei; Cui, Lei; Yin, Jingbo

doi:10.1021/acsami.8b02448

Cited by 48 publications

(36 citation statements)

References 60 publications

(96 reference statements)

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“…The main mechanism is thought to lie in Sr ion possessing the ability not only to enhance the osteogenesis differentiation of MSCs via phosphating the Ras/MAPK signaling pathway but also to suppress the osteoclastogenic differentiation by inhibiting RANKL expression in MSCs [54]. Investigators had also indicated that Sr-contained HA cement and scaffolds could promote early angiogenesis after implantation into the critical size of bone defect and significantly enhance new bone regeneration [55,56].…”

Section: Resultsmentioning

confidence: 99%

Effect of Strontium Substitution on the Physicochemical Properties and Bone Regeneration Potential of 3D Printed Calcium Silicate Scaffolds

Chiu

Shie

Lin

et al. 2019

IJMS

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In this study, we synthesized strontium-contained calcium silicate (SrCS) powder and fabricated SrCS scaffolds with controlled precise structures using 3D printing techniques. SrCS scaffolds were shown to possess increased mechanical properties as compared to calcium silicate (CS) scaffolds. Our results showed that SrCS scaffolds had uniform interconnected macropores (~500 µm) with a compressive strength 2-times higher than that of CS scaffolds. The biological behaviors of SrCS scaffolds were assessed using the following characteristics: apatite-precipitating ability, cytocompatibility, proliferation, and osteogenic differentiation of human mesenchymal stem cells (MSCs). With CS scaffolds as controls, our results indicated that SrCS scaffolds demonstrated good apatite-forming bioactivity with sustained release of Si and Sr ions. The in vitro tests demonstrated that SrCS scaffolds possessed excellent biocompatibility which in turn stimulated adhesion, proliferation, and differentiation of MSCs. In addition, the SrCS scaffolds were able to enhance MSCs synthesis of osteoprotegerin (OPG) and suppress macrophage colony-stimulating factor (M-CSF) thus disrupting normal bone homeostasis which led to enhanced bone formation over bone resorption. Implanted SrCS scaffolds were able to promote new blood vessel growth and new bone regeneration within 4 weeks after implantation in critical-sized rabbit femur defects. Therefore, it was shown that 3D printed SrCS scaffolds with specific controllable structures can be fabricated and SrCS scaffolds had enhanced mechanical property and osteogenesis behavior which makes it a suitable potential candidate for bone regeneration.

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Section: Resultsmentioning

confidence: 99%

Effect of Strontium Substitution on the Physicochemical Properties and Bone Regeneration Potential of 3D Printed Calcium Silicate Scaffolds

Chiu

Shie

Lin

et al. 2019

IJMS

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“…Porous microcarriers were fabricated from the nanocomposites of strontium-substituted hydroxyapatite-graft-poly (γ-benzyl L-glutamate) [103]. It was confirmed by confocal microscopy that the seeded rabbit adipose-derived stem cells (ADSCs) adhered and infiltrated within the internal pores of the microspheres.…”

Section: Understanding Of Real 3d Cell Culture and Challengesmentioning

confidence: 94%

Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

Huang

Abdalla

Xiao

et al. 2020

IJMS

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The concept of three-dimensional (3D) cell culture has been proposed to maintain cellular morphology and function as in vivo. Among different approaches for 3D cell culture, microcarrier technology provides a promising tool for cell adhesion, proliferation, and cellular interactions in 3D space mimicking the in vivo microenvironment. In particular, microcarriers based on biopolymers have been widely investigated because of their superior biocompatibility and biodegradability. Moreover, through bottom-up assembly, microcarriers have opened a bright door for fabricating engineered tissues, which is one of the cutting-edge topics in tissue engineering and regeneration medicine. This review takes an in-depth look into the recent advancements of microcarriers based on biopolymers—especially polysaccharides such as chitosan, chitin, cellulose, hyaluronic acid, alginate, and laminarin—for 3D cell culture and the fabrication of engineered tissues based on them. The current limitations and potential strategies were also discussed to shed some light on future directions.

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“…Recently, the field of bone tissue engineering (BTE) has been developing rapidly, and various promising biomaterials have emerged and have been widely applied for bone repair and regeneration (11,12). The use of osteogenic cells combined with various growth factors and biocompatible scaffolds can greatly promote bone repair.…”

Section: Introductionmentioning

confidence: 99%

The effect of enhanced bone marrow in conjunction with 3D-printed PLA-HA in the repair of critical-sized bone defects in a rabbit model

Liu¹,

Chu²,

Zhang³

et al. 2021

Ann Transl Med

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Background: Traditionally, the iliac crest has been the most common harvesting site for autologous bone grafts; however, it has some limitations, including poor bone availability and donor-site morbidity. This study sought to explore the effect of enhanced bone marrow (eBM) in conjunction with three-dimensional (3D)printed polylactide-hydroxyapatite (PLA-HA) scaffolds in the repair of critical-sized bone defects in a rabbit model.Methods: First, 3D-printed PLA-HA scaffolds were fabricated and evaluated using micro-computed tomography (μCT) and scanning electron microscopy (SEM). Twenty-seven New Zealand white rabbits were randomly divided into 3 groups (n=9 per group), and the defects were treated using 3D-printed PLA-HA scaffolds (the PLA-HA group) or eBM in conjunction with 3D-printed PLA-HA scaffolds (the PLA-HA/eBM group), or were left untreated (the control group). Radiographic, μCT, and histological analyses were performed to evaluate bone regeneration in the different groups.Results: The 3D-printed PLA-HA scaffolds were cylindrical, and had a mean pore size of 500±47.1 μm and 60%±3.5% porosity. At 4 and 8 weeks, the lane-sandhu X-ray score in the PLA-HA/eBM group was significantly higher than that in the PLA-HA group and the control group (P<0.01). At 8 weeks, the μCT analysis showed that the bone volume (BV) and bone volume/tissue volume (BV/TV) in the PLA-HA/ eBM group were significantly higher than those in the PLA-HA group and the control group (P<0.01).Hematoxylin and eosin staining indicated that the new bone area in the PLA-HA/eBM group was significantly higher than that in the PLA-HA group and the control group (P<0.01). Conclusions:The group that was treated with eBM in conjunction with 3D-printed PLA-HA showed enhanced bone repair compared to the other 2 groups. PLA-HA/eBM scaffolds represent a promising way to treat critical-sized bone defects.

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Nanocomposite Porous Microcarriers Based on Strontium-Substituted HA-g-Poly(γ-benzyl-l-glutamate) for Bone Tissue Engineering

Cited by 48 publications

References 60 publications

Effect of Strontium Substitution on the Physicochemical Properties and Bone Regeneration Potential of 3D Printed Calcium Silicate Scaffolds

Effect of Strontium Substitution on the Physicochemical Properties and Bone Regeneration Potential of 3D Printed Calcium Silicate Scaffolds

Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

The effect of enhanced bone marrow in conjunction with 3D-printed PLA-HA in the repair of critical-sized bone defects in a rabbit model

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