The effect of enhanced bone marrow in conjunction with 3D-printed PLA-HA in the repair of critical-sized bone defects in a rabbit model

Liu, Zhiqing; Chu, Wenxiang; Zhang, Linyuan; Zhai, Zanjing; Li, Fengxiang

doi:10.21037/atm-20-8198

Cited by 7 publications

(10 citation statements)

References 35 publications

(36 reference statements)

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“…At present various approaches have been established for the augmentation of critical-sized bone defects, which include autologous bone transplantation and the use of allografts [ 14 , 15 ]. Nevertheless, the aforementioned strategies had limitations that hindered their frequent use [ 16 ]. Bone graft alternatives offer physicians an “off the shelf” substitute to help heal defects.…”

Section: Discussionmentioning

confidence: 99%

“…We used bone graft, putty made with bone graft, and ADM to evaluate the critical bone defect model’s regenerative potential and osteoinductive nature. The outstanding bone regeneration and new bone formation in the segmental defect model indicate that bone grafts produced using the SCCO 2 technique improved the regenerative ability of bone grafts [ 16 ]. The bone graft produced using the SCCO 2 technique possessed the native pore structure of porcine bone with high porosity.…”

Section: Discussionmentioning

confidence: 99%

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Regenerative Efficacy of Supercritical Carbon Dioxide-Derived Bone Graft Putty in Rabbit Bone Defect Model

et al. 2022

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Bone defects can arise from numerous reasons, such as aging, tumor, trauma, infection, surgery, and congenital diseases. Bone grafts are commonly used as a substitute to fill the void and regenerate the defect. Due to its clean and green technology, the supercritical carbon dioxide (SCCO2) extraction aided the production of bone grafts is a recent trend. The SCCO2-derived bone graft has osteoconductive and osteoinductive properties along with excellent biocompatible, nontoxic, bioabsorbable, osteoconductive, and good mechanical properties; however, clinical usage during surgery is time-consuming. Therefore, we produced a putty material combining bone graft powder and acellular dermal matrix (ADM) powder and tested its regenerative efficacy in the critical defect in the rabbit model. The putty was found to retain the tubular structure. In addition, the putty depicted excellent stickiness and cohesiveness in both saline and blood medium. The bone regeneration of bone graft and putty was similar; both had excellent bone healing and regeneration of critical defects as evaluated by the X-ray, microtomography, hematoxylin-eosin, Masson trichrome, and alizarin red staining. Putty contains a less washout rate, good mechanical strength, and biocompatibility. In conclusion, the SCCO2-derived moldable putty could be a promising easy-to-use alternative for bone grafts at present which might have real-world usage in orthopedics as a potential bone void filler and dental socket preservation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Regenerative Efficacy of Supercritical Carbon Dioxide-Derived Bone Graft Putty in Rabbit Bone Defect Model

et al. 2022

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“…The rabbits were anesthetized by intramuscular injection with xylazine hydrochloride (0.025 ml/kg). According to some studies on critical radial defects [ 31 , 32 ],a longitudinal incision was created in the middle of the right radius to fully expose the radius, and a 15 mm long, 3 mm diameter cylindrical bone defect was created in the middle of the radius with a microvibrating saw. The SF/CS/nHA-BMSCs scaffold cell complexes were prepared as follows:The SF/CS/nHA scaffolds were prepared to a diameter of 3 mm and a length of 15 mm.Third-generation BMSCs were seeded on the scaffolds at a density of 2 × 10 5 /ml, and α-MEM containing 10% FBS was added.…”

Section: Methodsmentioning

confidence: 99%

A silk fibroin/chitosan/nanohydroxyapatite biomimetic bone scaffold combined with autologous concentrated growth factor promotes the proliferation and osteogenic differentiation of BMSCs and repair of critical bone defects

Zhou

Liu²,

She³

et al. 2022

Regenerative Therapy

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“…It is conducive to prepare excellent osteogenic scaffolds through a combination of a superior materials and effective growth factors. Common materials include inorganic materials such as hydroxyapatite (HA) [ 2 , 3 , 4 ], titanium alloy [ 5 , 6 ], and tricalcium phosphate (TCP) [ 7 , 8 ] and organic materials such as polylactic acid (PLA) [ 9 ], chitosan (CS) [ 10 , 11 ], collagen [ 12 ], and silk fibroin (SF) [ 13 , 14 , 15 ]. In early studies, these materials were often used alone, but in recent studies, two to three or even more materials have been combined.…”

Section: Introductionmentioning

confidence: 99%

“…In early studies, these materials were often used alone, but in recent studies, two to three or even more materials have been combined. Furthermore, biological scaffolds have prepared by various methods including layer-by-layer (LBL) self-assembly [ 16 , 17 ], 3D printing [ 4 , 6 , 7 , 9 ], coating [ 7 , 16 ] and electrospinning [ 13 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Angiogenesis-Based Scaffold of MesoporousBioactive Glass Nanofiber on Osteogenesis

Zheng

Bai

Huang

et al. 2022

IJMS

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There is still an urgent need for more efficient biological scaffolds to promote the healing of bone defects. Vessels can accelerate bone growth and regeneration by transporting nutrients, which is an excellent method to jointly increase osteogenesis and angiogenesis in bone regeneration. Therefore, we aimed to prepare a composite scaffold that could promote osteogenesis with angiogenesis to enhance bone defect repair. Here, we report that scaffolds were prepared by coaxial electrospinning with mesoporous bioactive glass modified with amino (MBG-NH2) adsorbing insulin-like growth factor-1 (IGF-1) as the core and silk fibroin (SF) adsorbing vascular endothelial growth factor (VEGF) as the shell. These scaffolds were named MBG-NH2/IGF@SF/VEGF and might be used as repair materials to promote bone defect repair. Interestingly, we found that the MBG-NH2/IGF@SF/VEGF scaffolds had nano-scale morphology and high porosity, as well as enough mechanical strength to support the tissue. Moreover, MBG-NH2 could sustain the release of IGF-1 to achieve long-term repair. Additionally, the MBG-NH2/IGF@SF/VEGF scaffolds could significantly promote the mRNA expression levels of osteogenic marker genes and the protein expression levels of Bmp2 and Runx2 in bone marrow mesenchymal stem cells (BMSCs). Meanwhile, the MBG-NH2/IGF@SF/VEGF scaffolds promoted osteogenesis by simulating Runx2 transcription activity through the phosphorylated Erk1/2-activated pathway. Intriguingly, the MBG-NH2/IGF@SF/VEGF scaffolds could also significantly promote the mRNA expression level of angiogenesis marker genes and the protein expression level of CD31. Furthermore, RNA sequencing verified that the MBG-NH2/IGF@SF/VEGF scaffolds had excellent performance in promoting bone defect repair and angiogenesis. Consistent with these observations, we found that the MBG-NH2/IGF@SF/VEGF scaffolds demonstrated a good repair effect on a critical skull defect in mice in vivo, which not only promoted the formation of blood vessels in the haversian canal but also accelerated the bone repair process. We concluded that these MBG-NH2/IGF@SF/VEGF scaffolds could promote bone defect repair under accelerating angiogenesis. Our finding provides a new potential biomaterial for bone tissue engineering.

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The effect of enhanced bone marrow in conjunction with 3D-printed PLA-HA in the repair of critical-sized bone defects in a rabbit model

Cited by 7 publications

References 35 publications

Regenerative Efficacy of Supercritical Carbon Dioxide-Derived Bone Graft Putty in Rabbit Bone Defect Model

Regenerative Efficacy of Supercritical Carbon Dioxide-Derived Bone Graft Putty in Rabbit Bone Defect Model

A silk fibroin/chitosan/nanohydroxyapatite biomimetic bone scaffold combined with autologous concentrated growth factor promotes the proliferation and osteogenic differentiation of BMSCs and repair of critical bone defects

The Effect of Angiogenesis-Based Scaffold of MesoporousBioactive Glass Nanofiber on Osteogenesis

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