Nanocomposite Materials Developed from Nano‐hydroxyapatite Impregnated Chitosan/κ‐Carrageenan for Bone Tissue Engineering.

Abstract

Herein, nano-hydroxyapatite impregnated chitosan/k-carrageenan (nHAp/CHI/k-CGN) nanocomposite was prepared through co-precipitation approach and was compared with nano-hydroxyapatite/chitosan (nHAp/CHI) nanocomposite for bone regeneration capability. The comparative assessment carried out through Scanning electron microscopy, Fourier transform infra-red spectroscopy, X-ray diffraction and mechanical testing revealed rough surface morphology, better interaction between the components, favorable crystallinity, and higher mechanical properties for the nHAp/CHI/k-CGN nanocomposite as compared to the bicomponent nanocomposite.Moreover, the in vitro biomineralization study showed increased deposition of apatite layer on nHAp/CHI/k-CGN nanocomposite as compared to nHAp/CHI nanocomposite. The cytocompatibility studies carried out using the MG-63 cell line revealed greater cell viability when cultured in the presence of nHAp/CHI/k-CGN nanocomposite as compared to nHAp/CHI. In addition, appropriate swelling ability, enhanced protein adsorption, and favorable degradation rate were also observed for the nHAp/CHI/k-CGN nanocomposite. All the results substantiate that nHAp/CHI/k-CGN nanocomposite could be a better candidate for bone regeneration.

Biocompatible nanocomposite scaffolds based on carrageenan incorporating hydroxyapatite and hesperidin loaded nanoparticles for bone tissue regeneration

“…Furthermore, the PHBV NPs‐CGN‐HA hydrogel showed higher efficiency in terms of promoting MG‐63 proliferation, as compared with the PHBV NPs‐CGN one ( p < 0.05), thus supporting the efficient role of HA in increasing the growth and development of osteoblasts, 44 which could be attributed to the osteoconductive nature of HA. In agreement with our findings, several scientists have also reported the greater viability of osteoblast cells for the scaffolds fabricated with HA 45,46 . Beside these, assessing the results of the viability of cells treated with drug solution and the HPN‐CGN hydrogel showed that the proliferation level of MG‐63 cells on the HPN‐CGN hydrogel was remarkably higher than that of the drug solution, thus confirming the osteoblastic stimulatory effect of both hydrogel material and HPN.…”

“…In agreement with our findings, several scientists have also reported the greater viability of osteoblast cells for the scaffolds fabricated with HA. 45,46 Beside these, assessing the results of the viability of cells treated with drug solution and the HPN-CGN hydrogel…”

Green synthesis of HAp/CS@β-CD nanocomposite and its applications for bone cell proliferation

“…This may indicate that the nano-rough surface can improve cell adhesion and growth for bone regeneration. Thus, the higher surface nano-roughness of the nanocomposite may facilitate greater cell adhesion and proliferation in it [25].…”

Fabrication of k-Carrageenan/Alginate/Carboxymethyl Cellulose basedScaffolds via 3D Printing for Potential Biomedical Applications

“…Alginate [ 22 ], chitosan [ 23 ], pectin [ 24 ], carboxymethyl cellulose [ 2 ], agarose [ 25 ] and k-carrageenan [ 26 ] are often used for their biological and physical properties that are vital for obtaining suitable scaffolds [ 16 , 27 ]. 3D printed hydrogels made of chitosan and pectin were assessed for lidocaine delivery in wound treatment; the results indicated that these materials represent proper candidates for wound dressings [ 24 ].…”