Green synthesis of HAp/CS@β-CD nanocomposite and its applications for bone cell proliferation

Abstract

In this study, nHAp/CS@β–CD–E nanocomposite has been synthesized by incorporating of chitosan (CS), β-cyclodextrin (β-CD), and Ficus pumila (L.) leaf extract with nano-hydroxyapatite (nHAp) via co-precipitation method. Meanwhile, the nHAp obtained from seashells as the starting material sources. The formation of nHAp crystalline phase structure, interfacial interactions, thermal stability and surface structural morphology of nanocomposite samples were characterized by physicochemical methods including XRD, FT-IR, TGA/DTA and SEM, respectively. Investigation of osteoblast cell proliferation activity for the MT3C3-osteoblast cell line was carried out using the MMT method. The results showed that nHAp/CS@β–CD biocomposite has osteoblastic cell proliferation activity, in which the composite contains the Ficus pumila (L.) leaf extract has higher activity than composite without extract. This research has new prospects in the field of biomedical material research.