Nanocapsulated Rift Valley Fever Vaccine Candidates and Relative Immunological and Histopathological Reactivity in Out Bred Swiss Mice

el-Razek, Noha Emad El-Din Abd; Shoman, Sahar; Mohamed, Aly F.

doi:10.4172/2157-7560.1000115

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Contrary to our data, a study reported that AA and formalin nonadjuvanted vaccines showed a higher antibody level compared with that detected post immunization with β -PL-inactivated vaccine where the oxidation of viral proteins is the inactivating mechanism of AA [26].…”

Section: Discussioncontrasting

confidence: 99%

“…Accordingly, current study aimed to evaluate the use of HemaGel to enhance the long-acting stability of locally prepared β -PL- and AA-inactivated rabies vaccines as well as its stabilization efficacy under thermal preservation conditions. HemaGel was used as plasma expander to avoid adverse effects of intact gelatin [17] and was utilized as stabilizer [12] to Rift Valley fever Vaccine (RVFV) [26]. This study showed that HemaGel can induce a higher level of RVFV stability and higher long-lasting antibody levels when added to Binary Ethyleneimine- (BEI-) inactivated RVFV than β -PL-inactivated vaccine.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Different Stabilizers and Inactivating Compounds for the Enhancement of Vero Cell Rabies Vaccine Stability and Immunogenicity:In VitroStudy

Olayan

El‐Khadragy

Mohamed³

et al. 2019

BioMed Research International

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Inactivation of rabies virus is essential for rabies vaccine preparation where the inactivating compound that is currently recommended for rabies vaccine preparation is β-propiolactone (β-PL). This compound is considered better than phenol and formalin but it is expensive and potentially carcinogenic. Data revealed that Ascorbic acid (AA) with cupric ions could yield complete and irreversible inactivation of rabies virus without adversely affecting its antigenicity. Additionally, the results of testing the vaccine potency with the selected inactivating compounds were comparable (P<0.05), and ED50 was higher than the recommended World Health Organization (WHO) limits. The use of HemaGel (plasma substitute) for testing vaccine stabilization was compared with the currently used vaccine stabilizers (human albumin and lactose). HemaGel yielded better stability than the other tested stabilizers. Monitoring of cellular and humoral immune responses indicated that both the total IgG level against rabies vaccine and the IFN and IL5 levels obtained with the HemaGel-stabilized vaccines were higher than those obtained with human albumin- and lactose-stabilized vaccine candidates.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of Different Stabilizers and Inactivating Compounds for the Enhancement of Vero Cell Rabies Vaccine Stability and Immunogenicity:In VitroStudy

Olayan

El‐Khadragy

Mohamed³

et al. 2019

BioMed Research International

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“…Researchers are now indulged to apply nanotechnology in designing the prodrugs for better delivery of drugs to the targeted site (targeting being an publicized promise of nanotechnology) [47][48][49], to increase its reactivity, to increase its surface-volume ratio [50,51], to reduce the toxicity (for good safety and biocompatibility) [52] and also to retain their lifespan till it reaches the site of action [53][54][55][56][57][58].…”

Section: Prodrug As a Part Of Nanoworldmentioning

confidence: 99%

Cutting Edge Approach on Prodrug: Contrivance for Target Drug Delivery

Varsha¹,

Rao²,

Rao³

2011

JBB

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Drug administration procedures are very much vital and are considered to be the prime-most criteria during the drug design and its appliance. Scrutinizing this, drugs are designed in such a way, bringing some change in their persona like bioavailability and bioequivalence. One such approach called prodrug, first started in 1950's is still a fertile area of research because of it's insist persona. Prodrugs are the shrouded drugs which are one or two chemical or enzymatic steps away from the active parent drug. Present review delineates about the prodrug action, types of prodrugs, new prodrug therapies, nanotech allied prodrugs.

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“…They are not equally efficient for all antigens due to their restricted immune‐enhancement effects and local reactions such as redness, swelling and pain at the injection site, or systemic reactions such as fever, chills and body aches associated with unfavourable immunoglobulin E responses [ 4 , 5 , 6 ]. Considering the above‐mentioned weak points for alum‐based adjuvants, biodegradable NPs as an adjuvant were developed by numerous procedures [ 7 , 8 , 9 , 10 , 11 ]. One of the important challenges in therapeutics is to deliver drug to the target site and preserve the necessary dosage in a sufficient period to achieve the desired medical outcome [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Investigating preparation and characterisation of diphtheria toxoid‐loaded on sodium alginate nanoparticles

Aghamiri

Noofeli

Saffarian

et al. 2022

IET Nanobiotechnology

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This paper aims to investigate the preparation and characterisation of the alginate nanoparticles (NPs) as antigen delivery system loaded by diphtheria toxoid (DT). For this purpose, both the loading capacity (LC) and Loading efficiency (LE) of the alginate NPs burdened by DT are evaluated. Moreover, the effects of different concentrations of sodium alginate and calcium chloride on the NPs physicochemical characteristics are surveyed in addition to other physical conditions such as homogenization time and rate. To do so, the NPs are characterised using particle size and distribution, zeta potential, scanning electron microscopy, encapsulation efficiency, in vitro release study and FT-IR spectroscopy. Subsequently, the effects of homogenization time and rate on the NPs are assessed. At the meantime, the NPs LC and efficiency in several DT concentrations are estimated. The average size of the NPs was 400.7 and 276.6 nm for unloaded and DT loaded, respectively. According to the obtained results, the zeta potential of the blank and DT loaded NPs are estimated as −23.7 mV and −21.2 mV, respectively. Whereas, the LC and LE were >80% and >90%, in that order. Furthermore, 95% of the releasing DT loaded NPs occurs at 140 h in the sustained mode without any bursting release. It can be concluded that the features of NPs such as morphology and particle size are strongly depended on the calcium chloride, sodium alginate concentrations and physicochemical conditions in the NPs formation process. In addition, appropriate concentrations of the sodium alginate and calcium ions would lead to obtaining the desirable NPs formation associated with the advantageous LE, LC (over 80%) and sustained in vitro release profile. Ultimately, the proposed NPs can be employed in vaccine formulation for the targeted delivery, controlled and slow antigen release associated with the improved antigen stability.

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Nanocapsulated Rift Valley Fever Vaccine Candidates and Relative Immunological and Histopathological Reactivity in Out Bred Swiss Mice

Cited by 7 publications

References 25 publications

Evaluation of Different Stabilizers and Inactivating Compounds for the Enhancement of Vero Cell Rabies Vaccine Stability and Immunogenicity:In VitroStudy

Evaluation of Different Stabilizers and Inactivating Compounds for the Enhancement of Vero Cell Rabies Vaccine Stability and Immunogenicity:In VitroStudy

Cutting Edge Approach on Prodrug: Contrivance for Target Drug Delivery

Investigating preparation and characterisation of diphtheria toxoid‐loaded on sodium alginate nanoparticles

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