Nanobiosensor-Based Diagnostic System: Transducers and Surface Materials

Dutta, Gorachand

doi:10.1007/978-981-32-9840-8_1

Cited by 10 publications

(3 citation statements)

References 54 publications

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“…Biological receptors are recognition elements that can be enzymes, proteins, antibodies, nucleic acids, cells, tissues, or receptor molecules [184]. These molecules are responsible for giving specificity to the biosensor and must be in direct contact with the transducer [179,185]. Depending on the recognition element, biosensors can be catalytic, including enzymes, microorganisms, organelles, cells, or tissues and based on affinity, including antibodies, nucleic acids, proteins, peptides, and aptamers [176,177].…”

Section: Monitoring Signatures Of Biomarkers With Biosensors In the C...mentioning

confidence: 99%

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Vásquez

Orozco

2022

Anal Bioanal Chem

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As a more efficient and effective way to address disease diagnosis and intervention, cutting-edge technologies, devices, therapeutic approaches, and practices have emerged within the personalized medicine concept depending on the particular patient's biology and the molecular basis of the disease. Personalized medicine is expected to play a pivotal role in assessing disease risk or predicting response to treatment, understanding a person's health status, and, therefore, health care decision-making. This work discusses electrochemical biosensors for monitoring multiparametric biomarkers at different molecular levels and their potential to elucidate the health status of an individual in a personalized manner. In particular, and as an illustration, we discuss several aspects of the infection produced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a current health care concern worldwide. This includes SARS-CoV-2 structure, mechanism of infection, biomarkers, and electrochemical biosensors most commonly explored for diagnostics, prognostics, and potentially assessing the risk of complications in patients in the context of personalized medicine. Finally, some concluding remarks and perspectives hint at the use of electrochemical biosensors in the frame of other cutting-edge converging/emerging technologies toward the inauguration of a new paradigm of personalized medicine. Graphical abstract

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Section: Monitoring Signatures Of Biomarkers With Biosensors In the C...mentioning

confidence: 99%

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Vásquez

Orozco

2022

Anal Bioanal Chem

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“…Moreover, an electrochemical signal of a redox-active species can be amplified using electrochemical redox cycling when its oxidized and reduced forms are stable. In particular, electrochemical-chemical (EC) redox cycling is well-suited for the simple and sensitive detection of excreted redox-active species [28,[36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid detection of Alternaria alternata using an excreted redox‐active species

et al. 2023

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Airborne fungi pose a serious threat to public health. Alternaria alternata (A. alternata) is a fungus that has been associated with the development of asthma. Detection using redox‐active species excreted from fungi is an effective method for a simple electrochemical fungal biosensor. The achievable electrochemical signal in most fungi, however, is exceptionally low because of the low amount of excreted redox‐active species and their slow excretion rates. Herein, we report that A. alternata excretes an exceptionally large amount of a redox‐active species that can be used for sensitive and selective detection of A. alternata. The excretion rate is enhanced in Tris buffer, and the electrochemical‐chemical redox cycling involving excreted redox‐active species significantly increases the electrochemical signals. Only A. alternata among five common airborne fungi provides large electrochemical signals, which allows selective detection of A. alternata. The calculated detection limit for A. alternata is ~20 spores/mL with an incubation period of 10 min, indicating that the detection method is highly sensitive and rapid. The detection method does not require complicated procedures or harsh pretreatment and is optimal for point‐of‐care testing of A. alternata.

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“…The possibility of detecting these analytes via non-invasive methods using readily available sweat can be made possible by designing these bioreceptors for speci c analytes. The advent of nano-biosensing [15][16][17][18] combined with various electrochemistry methods and arti cial intelligence to interpret the results has made it possible to realize such biosensors. On-going research on such point-of-care sensors is targeted towards improving their sensitivity and selectivity by testing the various bioreceptor combinations.…”

Section: Introductionmentioning

confidence: 99%

An insilico design of a peptide bioreceptor for cortisol using molecular modelling techniques

Deshpande,

De,

Badhe

et al. 2023

Preprint

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Cortisol is established as a reliable biomarker for stress prompting intensified research in developing wearable sensors to detect it via eccrine sweat. Since cortisol is present in sweat in trace quantities, typically 8-140ng/mL, developing such biosensors necessitates the design of bioreceptors with appropriate sensitivity and selectivity. In this work, we present a systematic methodology and semi-automated high -throughput screening tool for candidate bioreceptors from protein databases, via molecular docking, ranking them according to their binding affinities by conducting automated AutoDock Vina scoring simulations and finally validation via full atomistic steered molecular dynamics computations including umbrella sampling to estimate the potential of mean force using GROMACS. These explicit molecular dynamic calculations are carried out in eccrine sweat environment taking into consideration the protein dynamics and solvent effects. Subsequently, we present a candidate baseline peptide bioreceptor selected as a continuous sequence of amino acids favourably interacting with the target ligand i.e., cortisol from the active binding site of the proteins and maintaining its tertiary structure. A unique cysteine residue introduced at the N-terminus allows orientation-specific surface immobilization of the peptide onto the gold electrodes and to ensure exposure of the binding site. Comparative binding affinity simulations of this peptide with the target ligand along with commonly interfering species e.g., progesterone, testosterone and glucose are also presented to demonstrate the validity of this proposed peptide as a candidate baseline bioreceptor for future cortisol biosensor development.

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Nanobiosensor-Based Diagnostic System: Transducers and Surface Materials

Cited by 10 publications

References 54 publications

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine

Simple and rapid detection of Alternaria alternata using an excreted redox‐active species

An insilico design of a peptide bioreceptor for cortisol using molecular modelling techniques

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