Nanobioprobe mediated DNA aptamers for explosive detection

Abstract

Specific nucleic acid aptamers using the microtiter plate based modified SELEX method against explosive trinitrotoluene are reported. Efficient partitioning of dsDNA was carried out using streptavidin labeled gold nanoprobes for the selection of specific aptamers. The selected binders having an affinity of ~10(-7) M were used in the newly developed electrochemical aptasensor, exhibiting a detection limit of around 1 ppb for trinitrotoluene.

Graphene, carbon nanotubes, zinc oxide and gold as elite nanomaterials for fabrication of biosensors for healthcare

“…See supplementary file, section 2.2.2. for more literature. Streptavidin labelled gold nanoprobe and specific nucleic acid aptamers was used for detection of TNT (Priyanka et al, 2014). More examples of GNP biosensor are provided in supplementary file, section 2.2.2.…”

Selection and Biosensor Application of Aptamers for Small Molecules

“…2,4,6-Trinitrotoluene (TNT) is a commonly used explosive material whose detection is necessary for public safety as well as environmental and water decontamination. Different aptamers against it were selected with biosensing applications in mind: Ehrentreich-Förster et al were the only ones up to now to use their sensor for the detection of TNT in soil, solvent and water samples (Ehrentreich-Förster et al, 2008 ); others developed a sandwich assay (Ho et al, 2012 ), an electrochemical (Priyanka et al, 2014 ), and a Förster resonance energy transfer (FRET)-based aptasensor, the latter of which combined the use of a TNT-aptamer and an antibody (Sabherwal et al, 2014 ), but none was tested in real samples.…”

Aptamer-Functionalized Hybrid Nanostructures for Sensing, Drug Delivery, Catalysis and Mechanical Applications

“…By the immobilization of aptamers on appropriate transducers, numerous electrochemical [ 16 , 17 , 18 , 19 ], photoelectrochemical [ 20 , 21 , 22 , 23 ], surface plasmon resonance (SPR) [ 24 , 25 , 26 ], microgravimetric quartz-crystal-microbalance (QCM) [ 27 , 28 , 29 , 30 ], magnetic [ 31 , 32 , 33 ] and acoustic [ 34 , 35 , 36 ] sensors were demonstrated. Different target analytes were sensed by aptamers, including low-molecular-weight compounds, such as cocaine [ 37 , 38 , 39 , 40 ], explosives [ 41 , 42 , 43 ], pesticides [ 44 , 45 ], toxins [ 46 ] and antibiotics [ 47 , 48 , 49 ], and macromolecules such as protein [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ] and biopolymers [ 58 , 59 , 60 ]. The specific recognition of metal ions by aptamers, e.g., Mg 2+ , Zn 2+ and Ni 2+ led to the assembly of catalytic nucleic acids, DNAzymes [ 61 , 62 , 63 ], capable of cleaving oligonucleotides or ligating nucleic acids.…”