Nanobeam X-ray fluorescence and diffraction computed tomography on human bone with a resolution better than 120 nm

Palle, Jonas; Wittig, Nina Kølln; Kubec, Adam; Niese, Sven; Rosenthal, Martin; Burghammer, Manfred; Grünewald, Tilman A.; Birkedal, Henrik

doi:10.1016/j.jsb.2020.107631

Cited by 19 publications

(17 citation statements)

References 47 publications

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“…We therefore hypothesized that the smaller crystallites observed by XRD-CT, result from inhibition of crystallite growth by, for example, osteopontin fragments . This would suggest that mineral crystals around osteocyte lacunae may also be expected to be smaller in size; the ability to test this hypothesis can be expected to emerge as $100 nm spatial resolution XRD studies become possible (Palle et al, 2020).…”

Section: Varying Mineral Properties Across Human Osteonal Bonementioning

confidence: 99%

“…The resolution of these measurements is ultimately limited by the available beam size and has only recently surpassed a mm (vide infra), which would be necessary to probe many of the potential variations in bone described above. Using nano-focused hard X-ray beams, a true 3D resolution of better than 120 nm has now been achieved in a multimodal experiment incorporating both diffraction and fluorescence information on bone (Palle et al, 2020).…”

Section: Bone Mineral: One Size Does Not Fit Allmentioning

confidence: 99%

See 1 more Smart Citation

Bone hierarchical structure: spatial variation across length scales

Wittig¹,

Birkedal²

2022

Acta Crystallogr B Struct Sci Cryst Eng Mater

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Bone is a complex hierarchical biomineralized material, which is special amongst biominerals because it is replete with cells, namely, osteocytes. While bone has been scrutinized for centuries, many questions remain open and new research hints that the ultrastructure of bone, encompassing both the bone matrix itself and the embedded cell network, is much more heterogeneous than hitherto realized. A number of these new findings have been made thanks to the enormous developments in X-ray imaging that have occurred in recent decades, and there is promise that they will also allow many of the remaining open questions to be addressed. X-ray absorption or phase imaging affords high three-dimensional (3D) resolution and allows traversing the length scales of bone all the way down to the fine details of the lacuno-canalicular network housing the osteocytes. Multimodal X-ray imaging provides combined information covering both the length scales defined by the size of the measured volume and tomographic resolution, as well as those probed by the signal that is measured. In X-ray diffraction computed tomography (XRD-CT), for example, diffraction signals can be reconstructed tomographically, which offers detailed information about the spatial variations in the crystallographic properties of the bone biomineral. Orientational information can be obtained by tensor tomography. The combination of both small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS) tensor tomography gives information on the orientation of bone nanostructure and crystals, respectively. These new technical developments promise that great strides towards understanding bone structure can be expected in the near future. In this review, recent findings that have resulted from X-ray imaging are highlighted and speculation is given on what can be expected to follow.

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Section: Varying Mineral Properties Across Human Osteonal Bonementioning

confidence: 99%

Section: Bone Mineral: One Size Does Not Fit Allmentioning

confidence: 99%

Bone hierarchical structure: spatial variation across length scales

Wittig¹,

Birkedal²

2022

Acta Crystallogr B Struct Sci Cryst Eng Mater

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“…The elegant use of XRF-CT and XRD-CT in combination achieved a spatial resolution better than 120 nm as demonstrated by Palle et al 9 in an experiment optimised for studies on nanostructured hierarchical materials such as biomineralised bone. The XRF/XRD-CT measurements were conducted at the ESRF ID13 beamline using an X-ray beam focused by Multilayer Laue Lenses to 32 Â 31 nm 2 (FWHM) at an X-ray energy of 12.7 keV and intensity of 1.25 Â 10 10 photons per s. The tomographic data sets were collected with an exposure time of 100 ms per pixel and over an angular range of 180 .…”

Section: Chemical Imaging Using X-ray Techniquesmentioning

confidence: 99%

Atomic spectrometry update – a review of advances in X-ray fluorescence spectrometry and its special applications

Vanhoof

Bacon

Fittschen

et al. 2021

J. Anal. At. Spectrom.

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“…Optimized beamlines for XRD-CT and similar experiments have been designed (Vaughan et al, 2020). As such experiments become more frequent (Palle et al, 2020;Wittig et al, 2019;Dong et al, 2021;Jacques et al, 2011;Vamvakeros et al, 2018Jensen et al, 2021), the speed of integration, i.e. the transformation of the data from pixel coordinates to azimuthal and scattering vector length coordinates, becomes a critical component in the data analysis pipeline.…”

Section: Introductionmentioning

confidence: 99%

Very large-scale diffraction investigations enabled by a matrix-multiplication facilitated radial and azimuthal integration algorithm: MatFRAIA

Jensen¹,

Christensen²,

Weninger³

et al. 2022

J Synchrotron Radiat

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As synchrotron facilities continue to generate increasingly brilliant X-rays and detector speeds increase, swift data reduction from the collected area detector images to more workable 1D diffractograms becomes of increasing importance. This work reports an integration algorithm that can integrate diffractograms in real time on modern laptops and can reach 10 kHz integration speeds on modern workstations using an efficient pixel-splitting and parallelization scheme. This algorithm is limited not by the computation of the integration itself but is rather bottlenecked by the speed of the data transfer to the processor, the data decompression and/or the saving of results. The algorithm and its implementation is described while the performance is investigated on 2D scanning X-ray diffraction/fluorescence data collected at the interface between an implant and forming bone.

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Nanobeam X-ray fluorescence and diffraction computed tomography on human bone with a resolution better than 120 nm

Cited by 19 publications

References 47 publications

Bone hierarchical structure: spatial variation across length scales

Bone hierarchical structure: spatial variation across length scales

Atomic spectrometry update – a review of advances in X-ray fluorescence spectrometry and its special applications

Very large-scale diffraction investigations enabled by a matrix-multiplication facilitated radial and azimuthal integration algorithm: MatFRAIA

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