Bone hierarchical structure: spatial variation across length scales

Wittig, Nina Kølln; Birkedal, Henrik

doi:10.1107/s2052520622001524

Cited by 11 publications

(8 citation statements)

References 52 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tomography-based diffraction techniques have recently advanced from 2D to 3D studies (Wittig & Birkedal, 2022;Birkbak et al, 2015). Here, the spatial resolution is limited by the X-ray beam size, typically in the range $1-10 mm, but in some instances spatial resolutions down to 120 nm have been reported (Palle et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Bone mineral properties and 3D orientation of human lamellar bone around cement lines and the Haversian system

Grünewald¹,

Johannes²,

Wittig³

et al. 2023

IUCrJ

Self Cite

View full text Add to dashboard Cite

Bone is a complex, biological tissue made up primarily of collagen fibrils and biomineral nanoparticles. The importance of hierarchical organization in bone was realized early on, but the actual interplay between structural features and the properties on the nanostructural and crystallographic level is still a matter of intense discussion. Bone is the only mineralized tissue that can be remodeled and, at the start of the formation of new bone during this process, a structure called a cement line is formed on which regular bone grows. Here, the orientational relationship of nanostructural and crystallographic constituents as well as the structural properties of both nanostructural and crystallographic constituents around cement lines and the Haversian system in human lamellar bone are investigated. A combination of small- and wide-angle X-ray scattering tensor tomography is employed together with diffraction tomography and synchrotron computed tomography to generate a multi-modal image of the sample. This work shows that the mineral properties vary as a function of the distance to the Haversian canal and, importantly, shows that the cement line has differing mineral properties from the surrounding lamellar bone, in particular with respect to crystallite size and degree of orientation. Cement lines make up a significant portion of the bone matrix despite their small size, hence the reported findings on an altered mineral structure, together with the spatial modulation around the Haversian canal, have implications for the formation and mechanics of bone.

show abstract

Section: Introductionmentioning

confidence: 99%

Bone mineral properties and 3D orientation of human lamellar bone around cement lines and the Haversian system

Grünewald¹,

Johannes²,

Wittig³

et al. 2023

IUCrJ

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bone is mainly composed of osteocytes, collagen fibers, and cellular matrix, which play an irreplaceable role in the metabolic activities and growth and development of the human body. [ 27 ] According to its different morphologies, human bone can be divided into long, short, flat, and irregular bones. [ 28 ] The middle bone of the lone bone is dense and connects the two end epiphyses, called the backbone.…”

Section: Relevant Bone Properties For the Generation Of Bone Organoidsmentioning

confidence: 99%

Section: Relevant Bone Properties For the Generation Of Bone Organoidsmentioning

confidence: 99%

“…The new bone is continuously calcified and strengthened by the promotion of trace elements and hormones. The mechanical strength is also [27] Copyright 2022, International Union of Crystallography. B) Biomimetic design of implants for cortical and cancellous bone.…”

Section: Steps In Bone Regenerationmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A Review of Advanced Biomaterials and Cells for the Production of Bone Organoid

Zhang

et al. 2023

Small Science

View full text Add to dashboard Cite

Rapid advancements in traditional bone tissue engineering have led to innovation in bone repair models and the resolution of insurmountable clinical issues like graft scarcity. The pathophysiological process of treating bone disease, however, is a multidimensional and multimodal regenerative regulatory mechanism that includes numerous immune, inflammatory, or metabolic responses related to the graft or the organism itself. Based on a 3D in vitro cell culture system that is remarkably identical to the body's bone tissue, the bone organoid is a biomimicking bone organ environment. It can accurately mimic the actual repair and regeneration condition in vivo because it shares the same physiological function, structure, morphology, and metabolic process as endogenous bone tissue. As a disruptive regenerative medicine technology, it has wide application prospects in the fields of organ development, gene editing, disease modeling, and precision therapy. Herein, the development process and physiological basis of different cell‐based bone organoids are reviewed, the current status of the application of different materials, cells, and construction methods for building bone organoids is described, and the prospects and challenges for the development of bone organoids in future medical fields is discussed.

show abstract

Bone Hierarchical Structure: Heterogeneity and Uniformity

Rodriguez‐Palomo,

Østergaard,

Birkedal

2023

Adv Funct Materials

View full text Add to dashboard Cite

Bone has a complex hierarchical structure with structural integration from nm to cm. The understanding of bone structure is developing rapidly due to improvements in available methodologies that allow unravelling structures across several length scales. These methods include advances in electron microscopy, in particular, focused ion beam scanning electron microscopy (FIB‐SEM), confocal laser scanning microscopy techniques, X‐ray imaging, X‐ray diffraction tomography (XRD‐CT), and tensor tomography (small angle X‐ray scattering tensor tomgraphy, SAXS‐TT and wide angle X‐ray scattering tensor tomgraphy, WAXS‐TT). Special emphasis is placed on the latter X‐ray techniques that are emerging into powerful tools. Through a review of selected recent results on the structure of the bone matrix as well as the lacuno‐canalicular network housing the osteocyte cells of bone, it is proposed that bone is more heterogeneous than typically described and that local variation in composition and crystallography may play a significant role in bone biology in health and disease.

show abstract

Bone hierarchical structure: spatial variation across length scales

Cited by 11 publications

References 52 publications

Bone mineral properties and 3D orientation of human lamellar bone around cement lines and the Haversian system

Bone mineral properties and 3D orientation of human lamellar bone around cement lines and the Haversian system

A Review of Advanced Biomaterials and Cells for the Production of Bone Organoid

Bone Hierarchical Structure: Heterogeneity and Uniformity

Contact Info

Product

Resources

About