Nano/micro technologies for delivering macromolecular therapeutics using poly(d,l-lactide-co-glycolide) and its derivatives

Mundargi, Raghavendra C.; Babu, V. Ramesh; Rangaswamy, Vidhya; Patel, Paresh N.; Aminabhavi, Tejraj M.

doi:10.1016/j.jconrel.2007.09.013

Cited by 954 publications

(600 citation statements)

References 104 publications

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“…In many studies to date, the influence of changing numerous process parameters -such as the amount of polymer and drug used, the time for preparation, and the selected excipients (different emulsifier or release modifier) -on particles produced via the double emulsion method has been described (Buske et al, 2012;Corrigan and Li, 2009;Cruz et al, 2011;Feczkó et al, 2011;Mundargi et al, 2008). However, for comparison of the potential of a polymer to act as an encapsulation material it is of utmost importance to keep the majority of process parameters unchanged.…”

Section: Results and Disussionmentioning

confidence: 99%

Impact of PEG and PEG- b -PAGE modified PLGA on nanoparticle formation, protein loading and release

Rietscher

Czaplewska

Majdanski

et al. 2016

International Journal of Pharmaceutics

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Section: Results and Disussionmentioning

confidence: 99%

Impact of PEG and PEG- b -PAGE modified PLGA on nanoparticle formation, protein loading and release

Rietscher

Czaplewska

Majdanski

et al. 2016

International Journal of Pharmaceutics

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“…Microparticles of PLGA have been widely studied for their use as drug and antigen delivery systems [3], [31] and [6]. These particulate systems are able to deliver drugs or antigens such as DNA, proteins, and peptides over prolonged periods of time, and induce strong immune responses against encapsulated antigens [13], which makes them attractive candidates for vaccine development.…”

Section: Discussionmentioning

confidence: 99%

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Gómez

Csaba

Fischer

et al. 2008

Journal of Controlled Release

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Surface modifications of poly(lactide-co-glycolide) microparticles with different polycationic electrolytes have mainly been studied for conjugation to antigens and/or adjuvants. However, the in vivo immunological effects of using surface charged particles have not been address yet. In this study, microparticles were coated or not with protamine, a cationic and arginine-rich electrolyte that confers microparticles with a positively surface charge. We then evaluated the potential of protamine-coatings to assist the induction of immune responses in mice. Interestingly, enhanced antibodies and T-cell responses were observed in mice treated with the coated particles. In vitro studies suggested that the improved immunological performance was mediated by an increased uptake. Indeed, protamine-coated particles that carried a plasmid were even internalised into non-phagocytic cells and to cause their transfection. These results open the way for further research into a novel technology that combines the use protamine for facilitated cell penetration of that and biodegradable microparticles for prolonged antigen or drug release.

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“…Novel approaches include use of polymeric NP (Richardson et al, 2001) and the careful choice of the encapsulating polymer provides a biodegradable and biocompatible formulation (Mundargi et al, 2008). Biodegradable polyesters are ideal precursors for making NP that encapsulate smaller peptides and proteins, leading to improved pharmacokinetic profiles and reduced frequency of administration (Chan et al, 2009), but they are not without difficulty (Takenaga et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Preparation and in vivo evaluation of insulin-loaded biodegradable nanoparticles prepared from diblock copolymers of PLGA and PEG

Haggag

Abdel-Wahab

Ojo

et al. 2016

International Journal of Pharmaceutics

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A B S T R A C TThe aim of this study was to design a controlled release vehicle for insulin to preserve its stability and biological activity during fabrication and release. A modified, double emulsion, solvent evaporation, technique using homogenisation force optimised entrapment efficiency of insulin into biodegradable nanoparticles (NP) prepared from poly (DL-lactic-co-glycolic acid) (PLGA) and its PEGylated diblock copolymers. Formulation parameters (type of polymer and its concentration, stabiliser concentration and volume of internal aqueous phase) and physicochemical characteristics (size, zeta potential, encapsulation efficiency, in vitro release profiles and in vitro stability) were investigated. In vivo insulin sensitivity was tested by diet-induced type II diabetic mice. Bioactivity of insulin was studied using Swiss TO mice with streptozotocin-induced type I diabetic profile. Insulin-loaded NP were spherical and negatively charged with an average diameter of 200-400 nm. Insulin encapsulation efficiency increased significantly with increasing ratio of co-polymeric PEG. The internal aqueous phase volume had a significant impact on encapsulation efficiency, initial burst release and NP size. Optimised insulin NP formulated from 10% PEG-PLGA retained insulin integrity in vitro, insulin sensitivity in vivo and induced a sustained hypoglycaemic effect from 3 h to 6 days in type I diabetic mice..

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Nano/micro technologies for delivering macromolecular therapeutics using poly(d,l-lactide-co-glycolide) and its derivatives

Cited by 954 publications

References 104 publications

Impact of PEG and PEG- b -PAGE modified PLGA on nanoparticle formation, protein loading and release

Impact of PEG and PEG- b -PAGE modified PLGA on nanoparticle formation, protein loading and release

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Preparation and in vivo evaluation of insulin-loaded biodegradable nanoparticles prepared from diblock copolymers of PLGA and PEG

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