Nano-Kinetics of Probe-Particles in Solution Visualized by a Pin-Fiber Video Scope

Suzuto, Masashi; Hirakawa, Yasuyuki; Ohnishi, Hitoshi; Tachino, Shinji; Shingaki, Tomoteru; Eyring, Edward M.; Masujima, Tsutomu

doi:10.2116/analsci.19.43

Cited by 7 publications

(5 citation statements)

References 10 publications

(5 reference statements)

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“…7 We have already reported observations of gold colloidal particles and living cells by this system. 8 In these reports, we confirm that this newly developed video microscope system has the potential to be a superior device for biological and biomedical applications.…”

Section: Introductionsupporting

confidence: 67%

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Observation and analysis of single DNA nano-kinetics by pin-fiber video scope

Hirakawa

Suzuto

Ohnishi

et al. 2003

Analyst

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The nano-kinetic movement of a single DNA molecule was observed and analyzed by a newly developed video-microscope system with an optical fiber, called a pin-fiber video scope. A single lambda-DNA molecule was put in focus using fiber-illumination, and the stretching and shrinking motion was measured. The molecule's kinetics were analyzed by numerical calculations and are discussed. A photocleavage phenomenon of DNA molecules was also visualized by the pin-fiber video scope. The new video-microscope system has the potential to observe and analyze the nano-kinetics of a single molecule.

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Section: Introductionsupporting

confidence: 67%

“…The PFVS system has several advantages as described in detail elsewhere. 8 These are controllability of the illumination area, two imaging modes, no scanning procedure, easy installation, and low cost. In the present application of DNA observation, the feature of controllability of the illumination area was mainly utilized.…”

Section: Pin-fiber Video Scope (Pfvs)mentioning

confidence: 99%

Observation and analysis of single DNA nano-kinetics by pin-fiber video scope

Hirakawa

Suzuto

Ohnishi

et al. 2003

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“…The authors found that interference patterns of laser speckles on a single human living cell was easily recognized using a ''pin-fiber video microscope'' system we had developed. 6,7) Although human tissue and plant tissue observations have been reported to date, [8][9][10] to our knowledge, this is the first report of a single living human cell observation by laser speckle microscopy. In the experiment, it was found that by comparing a living cell with a fixed (dead) cell, the fluctuation activities of their laser speckle patterns were completely different.…”

mentioning

confidence: 98%

“…The experimental setup was fundamentally identical to that in the pin-fiber video microscope system we had already reported. 6,7) Figure 1 shows a simple schematic of the apparatus. The sample on the stage of the microscope (Zeiss, Axioplan) was illuminated using a laser (Crystalaser, Model BCL-005-M, 5 mW, 473 nm, 0.1 nm linewidth, linear polarization) beam propagated through an optical fiber (Thorlabs, SM500).…”

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confidence: 99%

Laser Speckle Microscope for Instantaneous Determination of Condition of Single Living Cell

Hirakawa¹,

Hasegawa²,

Masujima³

2004

Jpn. J. Appl. Phys.

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“…In this report, we applied the video microscopic method to analyze the dynamics of drug transport into a single cell and further to submicrometer size (0.2-0.8 µm diameter) acidic granules in which drug molecules are specifically concentrated. This kinetic analysis at the nanometer scale is called 'nanokinetics' [19] and we think that an understanding of nanokineticis is important for nanomedicine.…”

mentioning

confidence: 99%