Laser Speckle Microscope for Instantaneous Determination of Condition of Single Living Cell

Abstract

amplifier and scaling equipment. The lirni will undertake the entire analysis if desired.An alternativc analysis, from foils consisting of neptunium, plutonium, and z3xU in boron shields, of bare and cadmium covered gold. and of sulphur, is available for monitoring nuclear reactor excursions.

Living Plant Observation by a Laser Speckle Microscopy

“…When the observation region in the leaf was changed, it was found that the laser speckle fluctuation on an edge of the leaf was more active than any other regions of the leaf and that the condition of the observed laser speckle on a living leaf was slightly different from that on human cells. [1][2][3] The images obtained by the laser speckle microscopy consist of static speckles and dynamic speckles which cause speckle fluctuations. 1,2,4) In this experiment, the static speckle came to the front and the dynamic speckle was posteriorly-located, while the dynamic speckle was mainly observed in the case of human cells.…”

“…[1][2][3] The images obtained by the laser speckle microscopy consist of static speckles and dynamic speckles which cause speckle fluctuations. 1,2,4) In this experiment, the static speckle came to the front and the dynamic speckle was posteriorly-located, while the dynamic speckle was mainly observed in the case of human cells. 5) This may be explained by the different cellular structure between plants and human or animals.…”

“…The speckle fluctuation had been processed with a computer program (MathWorks, MATLAB) utilizing fast Fourier transformation (FFT) in the case of human cell observations 1,2) ; however, it was necessary to prepare a special algorithm to evaluate the laser speckle fluctuation on the leaf because of the behaviour difference of the laser speckle between leaves and human cells. The scheme as shown in Fig.…”

“…The merit of this technique is the ease in obtaining information of organisms without any special preparations such as staining and gene transfection. 1,2) In this study, observation of a living plant was performed by the laser speckle microscopy. A living leaf was observed for half a day at intervals of one hour.…”

Imaging of nanoparticle-labeled stem cells using magnetomotive optical coherence tomography, laser speckle reflectometry, and light microscopy

“…In addition to MM-OCT and light microscopy, we use laser speckle reflectometry as a 2-D reference modality to investigate sample motion. 21 To that, the sample was illuminated in a darkfield manner by a collimated laser beam at 638 nm, which was generated by a fiber-coupled laser diode with 1 mW output power (Lasiris PTL-500-635-1-2.0, Coherent Canada) and a fiber collimator (Fig. 2).…”