Nano‐amylose‐2,3‐bis(3,5‐dimethylphenylcarbamate)‐silica hybrid sol immobilized on open tubular capillary column for capillary electrochromatography enantioseparation

Abstract

The chiral organic-inorganic hybrid materials can exhibit a high loading, and the chiral selector nanoparticles can create efficient stationary phases for open-tubular capillary electrochromatography (OT-CEC). Hence, a novel protocol for the preparation of an OT column coated with nano-amylose-2,3-bis(3,5-dimethylphenylcarbamate) (nano-ABDMPC)-silica hybrid sol through in situ layer-by-layer self-assembly method was developed for CEC enantioseparation. By controlling the assembly cycle number of nano-ABDMPC-silica hybrid sol, a homogeneous, dense and stable coating was successfully prepared, which was confirmed by SEM and elemental analysis. As the main parameter influencing the chiral separating effect, the nano-ABDMPC bearing 3-(triethoxysilyl)propyl residues concentration was investigated. The experimental results showed that 10.0 mg/mL nano-ABDMPC bearing 3-(triethoxysilyl)propyl residues coated OT capillary column possessed chiral recognition ability toward the six enantiomers (phenylalanine, tyrosine, tryptophan, phenethyl alcohol, 1-phenyl-2-propanol, and Tröger's base) at some of the different conditions tested. Additionally, the coated OT column revealed adequate repeatability concerning run-to-run, day-to-day and column-to-column. These results demonstrated the promising applicability of nano-ABDMPC-silica hybrid sol coated OT column in CEC enantioseparations.

Recent developments of monolithic and open‐tubular capillary electrochromatography (2017–2019)

“…Silica‐based materials continue to be utilized in separation science because of their good chemical stability, and diverse and accessible chemical modifications . Liu and co‐workers developed a single step in situ biphasic reaction to fabricate a silica‐based porous layer open tubular (PLOT) column, which could easily to control the stationary phase properties and achieve good performance of OT‐CEC separations of complicated peptide products.…”

Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products

“…Removal of the solvent gives the xerogel or aerogel and after a heating step the final solid structure 14 . Table 1 lists the compositions of the various sol–gel mixtures and conditions for gel formation, together with the characterization of the coating and application of the coated capillary 10 , 13 , 15 – 17 .…”