NaCl effects in Zea mays L. x Tripsacum dactyloides (L.) L. hybrid calli and plants

Abstract

High salt concentrations in soils negatively affect maize growth. Techniques such as remote hybridization and in vitro selection have been extensively used to accelerate breeding processes. In order to determine the usefulness of Tripsacum to improve salt tolerance in maize, the effects of NaCl, in vitro and in vivo, were evaluated in an intergeneric hybrid (MT) obtained from crossing Zea mays with Tripsacum dactyloides. Organogenic calli, induced from immature MT hybrid embryos, were exposed to different NaCl concentrations and the survival and regeneration percentages were calculated. Plants of the MT hybrid, obtained from the organogenic calli, were exposed to NaCl concentrations considered harmful for maize. The shoot dry weights of plants exposed to 250 mM NaCl did not show significant differences respect to the control ones. Although sodium content in shoots was incremented 2,5 fold, it was not toxic for this material. The MT hybrid showed better behavior, in vitro and in vivo, that maize genotypes exposed to similar conditions.High salt concentrations in soils negatively affect maize growth and, consequently, produce a large drop in yield (Pasternak et al. 1995). In many countries of the world soil salinity is a serious problem for agriculture and,

The effect of salt stress on the germination of maize (Zea mays L.) seeds and photosynthetic pigments

“…Rahman et al (2000) reported that maize cultivars were significantly more tolerant to salt stress at germination than at later stages of growth. In order to determine the usefulness of Tripsacum in improving salt tolerance in maize, and the effects of NaCl, in vitro and in vivo, Pesqueira et al, (2006) evaluated an intergeneric hybrid obtained from crossing Zea mays L. with Tripsacum dactyloides L.…”

Siderophore Producing Aspergillus spp as Bioinoculant for Enhanced Growth of Mung Bean

“…Intact root system was carefully uprooted to prevent breakage. The following parameters were recorded: Length of root and shoot (cm), Number of lateral roots, Number of leaves, Fresh weight of plant (g), Dry weight of plant (g) (Pesqeira et al, 2006).…”

Salinity-resistant plant growth promoting rhizobacteria ameliorates sodium chloride stress on tomato plants

“…The results of these experiments are presented as tolerance index (Ti) where Ti0RLs/ RLc, where RLs stands for root length of plant grown in the presence of salt and RLc stands for root length of plants grown in the absence of salt. Rate of salt transfer from soil to plant (Tf 0NaCl in plant/ NaCl in soil), known as transfer factor was also calculated on the basis of salt content in plant and salt content in soil (Pesqueira et al 2006).…”

“…This crushed plant material (20 mg) was then added to a mixture of HNO 3 and HClO 4 in the ratio of 87:13. The plant was then heated to 708C for about 4 h and then at 1008C for 3 h. This plant digest was then used for estimating absorbed Na through AAS (Shimadzu 600) (Pesqueira et al 2006).…”