NAA10 as a New Prognostic Marker for Cancer Progression

Kim, Sun Myung; Ha, Eunyoung; Kim, Jin‐Young; Cho, Chi-Heum; Shin, So Jin; Seo, Ji Hae

doi:10.3390/ijms21218010

Cited by 15 publications

(10 citation statements)

References 64 publications

(112 reference statements)

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“…In order to evaluate the therapeutic effects of OMVs-MSN-5-FU on the animal model of oral squamous cell carcinoma, the levels of Naa10 and lymphocyte subgroups in the peripheral blood were factored into. Naa10 is the only subunit that can be catalyzed in the N-acetyltransferase A complex, and it plays an important role in the cell biology process ( 22 ). Studies have shown that, Naa10 participates in the autophagy, apoptosis, and proliferation of tumor cells ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Biofilm Nano-Composite Drugs OMVs-MSN-5-FU on Cervical Lymph Node Metastases From Oral Squamous Cell Carcinoma

Huang

2022

Front. Oncol.

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This work was developed to the effects of biofilm composite nano-drug delivery system (OMVs-MSN-5-FU) on lymph node metastasis from oral squamous cell carcinoma. Mesoporous silica nanoparticles loaded with 5-FU (MSN-5-FU) were prepared first. Subsequently, the outer membrane vesicles (OMV) of Escherichia coli were collected to wrap MSN-5-FU, and then OMVs-MSN-5-FU was prepared. It was then immersed in artificial gastric juice and artificial intestinal juice to explore the drug release rate. Next, the effects of different concentrations of the nano-drug delivery systems on the proliferation activity of oral squamous carcinoma cell line KOSC-2 cl3-43 were analyzed. Tumor-bearing nude mice models were prepared by injecting human tongue squamous cell carcinoma cells Tca8113 into BALB/c-nu nude mice. They were injected with the OMVs-MSN-5-FU nano drug carrier system, and peri-carcinoma tissue and cervical lymph node tissue were harvested to observe morphological changes by Hematoxylin – eosin (HE) staining. The scanning electron microscope (SEM) results showed that all MSN, MSN-5-FU, OMV, and OMV-MSN-5-FU were spherical and uniformly distributed, with particle sizes of about 60nm, 80nm, 90nm, and 140nm, respectively. Among them, OMV had a directional core-shell structure. The cumulative drug release rates of artificial gastric juice in 48 hours were 61.2 ± 2.3% and 26.5 ± 3.1%, respectively. The 48 hours cumulative drug release rates of artificial intestinal juice were 70.5 ± 6.3% and 32.1 ± 3.8%, respectively. The cumulative release of MSN-5-FU was always higher than OMV-MSN-5-FU. The cumulative release of MSN-5-FU was always higher than OMV-MSN-5-FU. After injection of OMVS-MSN-5-FU, the number of cancer cells was significantly reduced and cervical lymph node metastasis was significantly controlled. HE staining results showed that OMVS-MSN-5-FU injection reduced the number of stained cells. Dense lymphocytes were clearly observed in the cortex of neck lymphocytes. The OMVs-MSN-5-FU drug delivery system can slow down the drug release rate, significantly inhibit the proliferation activity of oral squamous cancer cells, and control the metastasis of cancer cells to cervical lymph nodes.

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Section: Discussionmentioning

confidence: 99%

Effects of Biofilm Nano-Composite Drugs OMVs-MSN-5-FU on Cervical Lymph Node Metastases From Oral Squamous Cell Carcinoma

Huang

2022

Front. Oncol.

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“…As a controversial protein, NAA10 has been found to play different key roles in many cancer types. Compared with noncancer tissues, the overexpression of NAA10 is displayed in many cancer tissues and cells, such as breast cancer, lung cancer, hepatocellular carcinoma, colorectal cancer, osteosarcoma, oral squamous cell carcinoma, and prostate cancer [24], while low expression in breast cancer and lung cancer was also identified [14]. In the different cell context the multifunction of NAA10 is correlated with tumor invasiveness, progression, metastasis, cell cycle regulation, apoptosis, DNA damage, stress response, development, and disease [13].…”

Section: Discussionmentioning

confidence: 99%

“…Among these DEPs, NAA10 is a multifunctional protein involved in various cellular activities required for proliferation, differentiation, autophagy, and apoptosis [13]. As previously reported, NAA10 can act as a tumor suppressor or an oncoprotein in various malignancies, that could be rendered by cancer specific microenvironment [14]. However, to date, no study has revealed the function of NAA10 in ESCC patients.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ and two‐dimensional‐LC‐MS/MS reveal NAA10 is a potential biomarker in esophageal squamous cell carcinoma

Wang

Chen

Han

et al. 2022

Proteomics Clinical Apps

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Purpose: Esophageal squamous cell carcinoma (ESCC) is one of the most common and serious malignancies in China. However, the exact mechanisms of tumor progression are still unclear. Thus, identifying biomarkers for early diagnosis, prognostic and recurrence assessment of ESCC is necessary.Experimental Design: iTRAQ was used to identify differentially expressed proteins (DEPs) in tumor tissues. N-alpha-acetyltransferase 10 (NAA10) is confirmed and validated by immunohistochemistry and western blotting. Furthermore, the effects of NAA10 on TE-1 cells were detected by CCK-8, colonies formation, anchorageindependent growth in soft agar, migration and transwell assays. LinkedOmics was used to identify differential gene expression with NAA10 and to analyze Gene Ontology and KEGG pathways. Coexpression gene network was conducted by the STRING database and Cytoscape software (MCODE plug-in).Results: 516 DEPs were identified. NAA10 was downregulated in cancer tissues and selected for further confirmed. Furthermore, NAA10 can inhibit proliferation and tumorigenesis, and suppress migration and invasion of TE-1. Functional network analysis suggested that NAA10 regulates the ribosome pathways involving eight ribosomal proteins. Conclusion and ClinicalRelevance: These findings clearly demonstrated that NAA10 is a tumor suppressor and novel potential biomarker for ESCC, laying a foundation for further study of the role of NAA10 in carcinogenesis.

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“…NAA10 catalyzes acetylation and is involved in cell proliferation [ 28 ]. More importantly, the NAA10 expression has been found in tumors from various organs, including the urinary bladder, cervix, liver, bone, lung, breast, colon, and prostate [ 29 , 30 ]. However, the pathway by which NAA10 induces tumorigenesis varies depending on the target proteins in different cancer tissues.…”

Section: Discussionmentioning

confidence: 99%

A Multiomics Profiling Based on Online Database Revealed Prognostic Biomarkers of BLCA

Chen

2022

BioMed Research International

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Background. Bladder cancer (BLCA) is one of the most common urological malignancies globally, posing a severe threat to public health. In combination with protein-protein interaction (PPI) network analysis of proteomics, Gene Set Variation Analysis (GSVA) and “CancerSubtypes” package of R software for transcriptomics can help identify biomarkers related to BLCA prognosis. This will have significant implications for prevention and treatment. Method. BLCA data were downloaded from The Cancer Genome Atlas (TCGA) database and GEO database (GSE13507). GSVA analysis converted the gene expression matrix to the gene set expression matrix. “CancerSubtypes” classified patients into three subtypes and established a prognostic model based on differentially expressed gene sets (DEGSs) among the three subtypes. For genes from prognosis-related DEGSs, functional and pathway enrichment analyses and PPI network analysis were carried out. The Human Protein Atlas (HPA) database was used for validation. Finally, the proportion of tumor-infiltrating immune cells (TIICs) was determined using the CIBERSORT algorithm. Results. In total, 414 tumor samples and 19 adjacent-tumor samples were obtained from TCGA, with 145 samples belonging to subtype A, 126 samples belonging to subtype B, and 136 samples belonging to subtype C. Then, we identified 83 DEGSs and constituted a prognostic signature with two of them: “GSE1460_CD4_THYMOCYTE_VS_THYMIC_STROMAL_CELL_DN” and “MODULE_253.” Finally, five subnets of two PPI networks were established, and nine core proteins were obtained: CDH2, COL1A1, EIF2S2, PSMA3, NAA10, DNM1L, TUBA4A, KIF11, and KIF23. The HPA database confirmed the expression of the nine core proteins in BLCA tissues. Furthermore, EIF2S2, PSMA3, DNM1L, and TUBA4A could be novel BLCA prognostic biomarkers. Conclusions. In this study, we discovered two gene sets linked to BLCA prognosis. PPI analysis confirmed the network’s core proteins, and several newly discovered biomarkers of BLCA prognosis were identified.

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NAA10 as a New Prognostic Marker for Cancer Progression

Cited by 15 publications

References 64 publications

Effects of Biofilm Nano-Composite Drugs OMVs-MSN-5-FU on Cervical Lymph Node Metastases From Oral Squamous Cell Carcinoma

Effects of Biofilm Nano-Composite Drugs OMVs-MSN-5-FU on Cervical Lymph Node Metastases From Oral Squamous Cell Carcinoma

iTRAQ and two‐dimensional‐LC‐MS/MS reveal NAA10 is a potential biomarker in esophageal squamous cell carcinoma

A Multiomics Profiling Based on Online Database Revealed Prognostic Biomarkers of BLCA

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