Na+, K+-ATPase activity and ATP concentration in horses of the Wielkopolski breed in relation to age

Abstract

This study aimed at determining relationships between the age of the Wielkopolski horses, ATP in whole blood and in the erythrocytes, and between erythrocyte Na + , K + -ATPase activity, and serum concentrations of mineral components. ATP was measured in whole blood and in erythrocytes by HPLC method. Serum concentrations of Ca 2+ and Mg 2+ were measured spectrophotometrically, while Na + and K + by flame photometry. In horses aged from 4 to 48 months, a dynamic decrease in ATP activity was found. Erythrocyte Na + , K + -ATPase activity decreased proportionally with the decrease in ATP activity. The results of this study may enable planning physical effort of horses at optimum use of energetic efficiency of their erythrocytes and mineral components in relation to their age.