Na+, K+-ATPase Activating Antibody Displays in vitro and in vivo Beneficial Effects in the Pilocarpine Model of Epilepsy

Freitas, Mayara Lutchemeyer de; Oliveira, Clarissa Vasconcelos de; Mello, Fernanda Kulinski; Funck, Vinícius Rafael; Fighera, Michele Rechia; Royes, Luiz Fernando Freire; Furian, Ana Flávia; Larrick, James W.; Oliveira, Mauro Schneider

doi:10.1016/j.neuroscience.2018.02.044

Cited by 10 publications

(9 citation statements)

References 37 publications

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“…Brain’s use of oxygen and glucose is the fundamental point which this scan shows. This relation of seizure activity with glucose uptake and release of glutamate can be also be measured by fluorescent deoxyglucose and thus, is an important aspect for determination of seizure foci [39,40]. The advanced form of PET is SPECT, an imaging method using nuclear medicine which measures regional cerebral blood flow (rCBF); based on the principle that partial seizures are associated with an increase in rCBF [41].…”

Section: Analytical Tools For Epilepsy Detectionmentioning

confidence: 99%

Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Tiwari

Sharma

Mujawar

et al. 2019

Sensors

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Epilepsy is a serious neurological disorder which affects every aspect of patients’ life, including added socio-economic burden. Unfortunately, only a few suppressive medicines are available, and a complete cure for the disease has not been found yet. Excluding the effectiveness of available therapies, the timely detection and monitoring of epilepsy are of utmost priority for early remediation and prevention. Inability to detect underlying epileptic signatures at early stage causes serious damage to the central nervous system (CNS) and irreversible detrimental variations in the organ system. Therefore, development of a multi-task solving novel smart biosensing systems is urgently required. The present review highlights advancements in state-of-art biosensing technology investigated for epilepsy diseases diagnostics and progression monitoring or both together. State of art epilepsy biosensors are composed of nano-enabled smart sensing platform integrated with micro/electronics and display. These diagnostics systems provide bio-information needed to understand disease progression and therapy optimization timely. The associated challenges related to the development of an efficient epilepsy biosensor and vision considering future prospects are also discussed in this report. This review will serve as a guide platform to scholars for understanding and planning of future research aiming to develop a smart bio-sensing system to detect and monitor epilepsy for point-of-care (PoC) applications.

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Section: Analytical Tools For Epilepsy Detectionmentioning

confidence: 99%

Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Tiwari

Sharma

Mujawar

et al. 2019

Sensors

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“…In addition, the increase in the intracellular sodium concentration can lead to a secondary increase in sodium and calcium exchange, while calcium influx can lead to excitatory synaptic glutamate release and neurotoxicity and neuronal death secondary to calcium overload, 57 further increasing seizure susceptibility. Freitas, M.L.D., et al confirmed that the NA‐K‐ATPase activator DRRSAb reduced glutamate release in pilocarpine‐kindled mouse brain slices in vitro 60 . Funck, V.R., et al found that DRRSAb injection into the hippocampus of mice delayed pentylenetetrazol‐induced seizures and reduced their seizure susceptibility 61 .…”

Section: Chronic Hypoxia Increases Seizure Susceptibility By Changing...mentioning

confidence: 95%

“…Freitas, M.L.D., et al confirmed that the NA‐K‐ATPase activator DRRSAb reduced glutamate release in pilocarpine‐kindled mouse brain slices in vitro. 60 Funck, V.R., et al found that DRRSAb injection into the hippocampus of mice delayed pentylenetetrazol‐induced seizures and reduced their seizure susceptibility. 61 Krishnan, G.P., et al demonstrated that the sodium and potassium ATP enzyme blocker STDN can increase seizure susceptibility and prolong seizure times in experimental animals.…”

Section: Chronic Hypoxia Increases Seizure Susceptibility By Changing...mentioning

confidence: 99%

Relationship between chronic hypoxia and seizure susceptibility

Fan

2022

CNS Neurosci Ther

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Chronic hypobaric hypoxia in high-altitude areas can affect the functions of the heart, 1 cerebrovascular system, and respiratory system, impair cognition, accelerate the progression of neurodegenerative diseases, seriously affect the normal physiological function of body systems, and even cause systemic diseases. 2 Qinghai Province is located in northwestern China, with an average elevation of more than 3200 meters. Its natural hypobaric hypoxic environment provides a unique advantage for studying neurological diseases caused by chronic hypoxia. In recent years, with the increase in construction on the Qinghai-Tibet Plateau and the need for development in the western region of China, a large number of engineers, scholars, and other relevant personnel from low-altitude areas have entered the plateau, which is likely to cause prominent public health problems with regard to the neurological system in the plateau area of China.Therefore, the study of neurological diseases caused by chronic hypobaric hypoxic environments has broad prospects.To date, research investigating neurological diseases caused by chronic hypoxia in international academic research has mainly focuses on the fields of cerebrovascular diseases, brain cognitive function, and nervous system degenerative diseases. 3 However, few studies have investigated the relationship between chronic hypoxia and epilepsy. In addition, the treatment of epilepsy focuses on the control of symptoms after the onset of epilepsy, 4 and there is a lack of in-depth studies on the early prevention and treatment of epilepsy and the geographical characteristics of its onset. Acute hypoxia can lead to epileptic seizures, and its clinical manifestations

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“…Freitas ML et al incubated brain slices with DRRSAb and reported a subsequent glutamate release ex vivo. 64 Furthermore, activation of Na+-K+-ATPase using DRRSAb invivodecreasedseizuresusceptibilityinthepilocarpinemodelof epilepsy. 64 Funck et al 5 alsofoundthatDRRSAbwillincreasehippocampal Na+-K+-ATPase activity in mice and attenuate seizure susceptibilityinpost-SEanimalmodels.Theseresultsshowedthat the intervention of Na+-K+-ATPasewouldimpactepilepsy.…”

Section: Protectiveeffectsinepilepsyafterapplying Na+ -K+ -Atpaseacti...mentioning

confidence: 99%

“…Some researchers use Na+‐K+‐ATPase activating antibody DRRSAb to activate the ion pump. Freitas ML et al incubated brain slices with DRRSAb and reported a subsequent glutamate release ex vivo 64 . Furthermore, activation of Na+‐K+‐ATPase using DRRSAb in vivo decreased seizure susceptibility in the pilocarpine model of epilepsy 64 .…”

Section: Na+‐k+‐atpase and Seizures/epilepsy: Evidence From Bench And...mentioning

confidence: 99%

The role of Na⁺‐K⁺‐ATPasein the epileptic brain

et al. 2022

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It is essential for normal structure and function of all cell membranes. Itsprimaryfunctionsincludemaintenanceiongradientacrossmembranes 2-4 anduptakeandreleaseofneurotransmitters. 5-7 Therefore, Na+-K+-ATPaseplaysacrucialroleinionhomeostasisandcellular excitability. Dysfunction of Na+-K+-ATPasemayleadtomanytypes ofcentralnervoussystem(CNS)disorders,includingepilepsy. 8-11 Epilepsyisacommonneurologicaldisordercharacterizedbyrecurrentspontaneousseizures, 12 causedbythehighlysynchronized firingofneuronswithhyperexcitability. 13 It affects about 70 million population around the world. 14 Unfortunately, about one-third of epilepticpatientsremaindrug-resistant, 15 leadingtoasituationthat needsamoreeffectivedrugtarget.Anincreasingnumberofstudies unveiled a significant role of Na+-K+-ATPase in epilepsy. Notably, mutation of genes encoding Na+-K+-ATPase leads to epilepsy as partofitsphenotype.Inrodentmodelsofepilepsy,theactivityof Na+-K+-ATPasewasreportedtochangeaswell.Pharmacologicalinhibition of Na+-K+-ATPasewillcauseepilepticseizureinrodentsas well. Na+-K+-ATPaseactivatingantibody,bycontrast,wasreported tohaveaprotectiveeffectonepilepsy.However,discordantresults arenotuncommon,probablyduetodifferencesinetiologies,testing timing, features of various epilepsy models, etc. Hence, in this review,webrieflysummarizestructureandphysiologicalfunctionof Na+-K+-ATPaseintheCNS.Thenweaimtosummarizeandevaluate currentunderstandingsofNa+-K+-ATPaseandepilepsy,hopingto provideacomprehensiveandnovelviewontheroleofATPaseinthe epileptic brain and also therapeutic strategies associated with the

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Na+, K+-ATPase Activating Antibody Displays in vitro and in vivo Beneficial Effects in the Pilocarpine Model of Epilepsy

Cited by 10 publications

References 37 publications

Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Relationship between chronic hypoxia and seizure susceptibility

The role of Na⁺‐K⁺‐ATPasein the epileptic brain

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Na+, K+-ATPase Activating Antibody Displays in vitro and in vivo Beneficial Effects in the Pilocarpine Model of Epilepsy

Cited by 10 publications

References 37 publications

Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Relationship between chronic hypoxia and seizure susceptibility

The role of Na+‐K+‐ATPasein the epileptic brain

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The role of Na⁺‐K⁺‐ATPasein the epileptic brain