N6-methyladenosine regulates PEDV replication and host gene expression

Chen, Jianing; Jin, Li; Wang, Zemei; Wang, Liyuan; Chen, Qingbo; Cui, Yaru; Liu, Guangliang

doi:10.1016/j.virol.2020.06.008

Cited by 23 publications

(16 citation statements)

References 46 publications

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“…The m 6 A methyltransferases and demethylases are mainly localized in the nucleus. Infection by viruses that replicate in the cytoplasm, such as enterovirus 71 (EV71), HCV, ZIKV, and porcine epidemic diarrhea virus (PEDV), affects the expression and localization of methyltransferases and demethylases to facilitate their RNA m 6 A modifications, which influences viral replication ( 37 , 38 , 50 – 52 ). To check whether SARS-CoV-2 infection had a similar effect, SARS-CoV-2-infected Vero E6 cells were harvested.…”

Section: Resultsmentioning

confidence: 99%

“…Most RNA viruses that replicate in the cytoplasm, including ZIKV, West Nile virus, PEDV, and EV71, hijack the host m 6 A machinery to modify the RNA and therefore do not encode methyltransferase ( 37 , 38 , 50 , 52 ). SARS-CoV-2 nonstructural proteins NSP14 and NSP16 have methyltransferase function and play key roles in the m 7 G cap and 2′- O -methylation modification ( 59 – 61 ).…”

Section: Discussionmentioning

confidence: 99%

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Methyltransferase-like 3 Modulates Severe Acute Respiratory Syndrome Coronavirus-2 RNA N6-Methyladenosine Modification and Replication

Zhang

Hao

et al. 2021

mBio

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Methyltransferase-like 3 Modulates Severe Acute Respiratory Syndrome Coronavirus-2 RNA N6-Methyladenosine Modification and Replication

Zhang

Hao

et al. 2021

mBio

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“…As CHIKV, coronaviruses also produce subgenomic RNAs. The first identification of m 6 A modifications in a coronavirus was carried out in the porcine epidemic diarrhea virus (PEDV) [108], shortly followed by SARS-CoV-2 [91]. PEDV does not infect humans but causes high mortality associated with severe diarrhea and vomiting in piglets younger than one week old.…”

Section: A and Coronavirusesmentioning

confidence: 99%

From A to m6A: The Emerging Viral Epitranscriptome

Baquero-Pérez

Díez

2021

Viruses

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There are over 100 different chemical RNA modifications, collectively known as the epitranscriptome. N6-methyladenosine (m6A) is the most commonly found internal RNA modification in cellular mRNAs where it plays important roles in the regulation of the mRNA structure, stability, translation and nuclear export. This modification is also found in viral RNA genomes and in viral mRNAs derived from both RNA and DNA viruses. A growing body of evidence indicates that m6A modifications play important roles in regulating viral replication by interacting with the cellular m6A machinery. In this review, we will exhaustively detail the current knowledge on m6A modification, with an emphasis on its function in virus biology.

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“…However, the effects of m6A modifications can either promote viral replication as it is the case for enterovirus 71, influenza A virus and the human immunodeficiency virus, but it can also negatively impact viral production as demonstrated during both HCV and ZIKV infection ( 235 ). M6A modifications have been studied in cells infected with the α-CoV PEDV strain and the authors showed a decrease in demethylation activity that correlates with an increase of m6A addition in the host cellular RNAs resulting in a decrease of PEDV replication ( 236 ). The RNA genome of SARS-CoV-2 contains >50 potential m6A sites based on the presence of specific sequence motifs for m6A modification by the RNA methylase complex METTL3/14 ( 237 ).…”

Section: Discussionmentioning

confidence: 99%

Translational control of coronaviruses

Breyne

Vindry

Guillin

et al. 2020

Nucleic Acids Research

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Coronaviruses represent a large family of enveloped RNA viruses that infect a large spectrum of animals. In humans, the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is responsible for the current COVID-19 pandemic and is genetically related to SARS-CoV and Middle East respiratory syndrome-related coronavirus (MERS-CoV), which caused outbreaks in 2002 and 2012, respectively. All viruses described to date entirely rely on the protein synthesis machinery of the host cells to produce proteins required for their replication and spread. As such, virus often need to control the cellular translational apparatus to avoid the first line of the cellular defense intended to limit the viral propagation. Thus, coronaviruses have developed remarkable strategies to hijack the host translational machinery in order to favor viral protein production. In this review, we will describe some of these strategies and will highlight the role of viral proteins and RNAs in this process.

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N6-methyladenosine regulates PEDV replication and host gene expression

Cited by 23 publications

References 46 publications

Methyltransferase-like 3 Modulates Severe Acute Respiratory Syndrome Coronavirus-2 RNA N6-Methyladenosine Modification and Replication

Methyltransferase-like 3 Modulates Severe Acute Respiratory Syndrome Coronavirus-2 RNA N6-Methyladenosine Modification and Replication

From A to m6A: The Emerging Viral Epitranscriptome

Translational control of coronaviruses

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