“…Establishment of OIR mouse model C57BL/6J mice obtained from the SJA Laboratory Animal Center (Changsha, Hunan, China) were used in the study. The OIR model was established as previously described [8,9]. Pups were exposed to hyperoxic conditions (75%) on postnatal day (P) 7, and moved back to room air at P12.…”
Section: Methodsmentioning
confidence: 99%
“…As the most prevalent mRNA modification in mammals [4], N6-methyladenosine (m 6 A) RNA methylation exerts potential epigenetic functions in various physiological and pathological processes [5][6][7]. We recently revealed the profiles of altered m 6 A epitranscriptomes of mRNAs and long non-coding RNAs in the retinas of oxygen-induced retinopathy (OIR) [8], a classical mouse model for retinal neovascular diseases [9]. Several studies suggested the regulatory roles of m 6 A modifications in retinal neovascular diseases.…”
Ischemia-induced pathological neovascularization in the retina is a leading cause of blindness in various age groups. The purpose of the current study was to identify the involvement of circular RNAs (circRNAs) methylated by N
6
-methyladenosine (m
6
A), and predict their potential roles in oxygen-induced retinopathy (OIR) in mice. Methylation assessment via microarray analysis indicated that 88 circRNAs were differentially modified by m
6
A methylation, including 56 hyper-methylated circRNAs and 32 hypo-methylated circRNAs. Gene ontology enrichment analysis predicted that the enriched host genes of the hyper-methylated circRNAs were involved in cellular process, cellular anatomical entity, and protein binding. Host genes of the hypo-methylated circRNAs were enriched in the regulation of cellular biosynthetic process, the nucleus, and binding. According to the Kyoto Encyclopedia of Genes and Genomes analysis, those host genes were involved in the pathways of selenocompound metabolism, salivary secretion, and lysine degradation. MeRIP-qPCR verified significant alterations in m
6
A methylation levels of mmu_circRNA_33363, mmu_circRNA_002816, and mmu_circRNA_009692. In conclusion, the study revealed the m
6
A modification alterations in OIR retinas, and the findings above shed light on the potential roles of m
6
A methylation in circRNA regulatory functions in the pathogenesis of ischemia-induced pathological retinal neovascularization.
“…Establishment of OIR mouse model C57BL/6J mice obtained from the SJA Laboratory Animal Center (Changsha, Hunan, China) were used in the study. The OIR model was established as previously described [8,9]. Pups were exposed to hyperoxic conditions (75%) on postnatal day (P) 7, and moved back to room air at P12.…”
Section: Methodsmentioning
confidence: 99%
“…As the most prevalent mRNA modification in mammals [4], N6-methyladenosine (m 6 A) RNA methylation exerts potential epigenetic functions in various physiological and pathological processes [5][6][7]. We recently revealed the profiles of altered m 6 A epitranscriptomes of mRNAs and long non-coding RNAs in the retinas of oxygen-induced retinopathy (OIR) [8], a classical mouse model for retinal neovascular diseases [9]. Several studies suggested the regulatory roles of m 6 A modifications in retinal neovascular diseases.…”
Ischemia-induced pathological neovascularization in the retina is a leading cause of blindness in various age groups. The purpose of the current study was to identify the involvement of circular RNAs (circRNAs) methylated by N
6
-methyladenosine (m
6
A), and predict their potential roles in oxygen-induced retinopathy (OIR) in mice. Methylation assessment via microarray analysis indicated that 88 circRNAs were differentially modified by m
6
A methylation, including 56 hyper-methylated circRNAs and 32 hypo-methylated circRNAs. Gene ontology enrichment analysis predicted that the enriched host genes of the hyper-methylated circRNAs were involved in cellular process, cellular anatomical entity, and protein binding. Host genes of the hypo-methylated circRNAs were enriched in the regulation of cellular biosynthetic process, the nucleus, and binding. According to the Kyoto Encyclopedia of Genes and Genomes analysis, those host genes were involved in the pathways of selenocompound metabolism, salivary secretion, and lysine degradation. MeRIP-qPCR verified significant alterations in m
6
A methylation levels of mmu_circRNA_33363, mmu_circRNA_002816, and mmu_circRNA_009692. In conclusion, the study revealed the m
6
A modification alterations in OIR retinas, and the findings above shed light on the potential roles of m
6
A methylation in circRNA regulatory functions in the pathogenesis of ischemia-induced pathological retinal neovascularization.
“…1 ). For example, as a classical model for retinal angiogenesis, a mouse model of oxygen-induced retinopathy (OIR) shows significant differences of m 6 A modification in mRNA, circRNA, and lncRNA compared to the control group [ 25 , 26 ]. Fig.…”
Section: A Modification In Ophthalmic Diseasesmentioning
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