N,N-dimethyl phytosphingosine induces caspase-8-dependent cytochrome c release and apoptosis through ROS generation in human leukemia cells

Kim, Byeong Mo; Choi, Yun Jung; Han, Young‐Soo; Yun, Yeon-Sook; Hong, Sung Hee

doi:10.1016/j.taap.2009.05.020

Cited by 57 publications

(45 citation statements)

References 42 publications

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“…It is reported that quercetin also has antiapoptotic function (Chao et al, 2009;Kim et al, 2009). Our previous studies have shown that quercetin has effectively inhibited pro-apoptotic member Bax and up-regulated anti-apoptotic member Bcl-XL (Bu et al, 2011;Jia et al, 2011).…”

Section: Discussionmentioning

confidence: 97%

Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Jia

Lin

et al. 2012

J. Zhejiang Univ. Sci. B

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Abstract:As a component of diesel exhaust particles, 3-methyl-4-nitrophenol (4-nitro-m-cresol, PNMC) is also a metabolite of the insecticide fenitrothion and imposes hazardous effects on human health. In the present study, the alleviative effect of a common antioxidant flavonoid quercetin on mouse germ cells intoxicated by PNMC was investigated. Results showed that a single intraperitoneal injection of PNMC at 100 mg/kg induced severe testicular damage after one week. PNMC-treated mice showed a significant loss of germ cells (approximate 40% loss of round germ cells). PNMC caused an increase of hydroxyl radical and hydrogen peroxide production and lipid peroxidation, as well as a decrease in glutathione level, superoxide dismutase and glutathione peroxidase activities. Furthermore, treatment of PNMC increased expression of the pro-apoptotic protein Bax and decreased expression of the anti-apoptotic protein Bcl-XL in germ cells. In addition, testicular caspase-3 activity was significantly up-regulated and germ cell apoptosis was significantly increased in the PNMC-treated mice. In contrast, combined administration of quercetin at 75 mg/kg significantly attenuated PNMC-induced testicular toxicity. These results indicate that the antioxidant quercetin displays a remarkable protective effect on PNMC-induced oxidative damage in mouse testes and may represent an efficient supplement to attenuate reproductive toxicity by environmental toxicants to ensure healthy sperm production.

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Section: Discussionmentioning

confidence: 97%

Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Jia

Lin

et al. 2012

J. Zhejiang Univ. Sci. B

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“…Because there is no evidence of the extrinsic apoptosis pathway or the simultaneous activation of caspase 9, it is possible that a caspase 8-Bid-Bax pathway can be triggered by ROS as in N,N-dimethyl phytosphingosine-treated leukemia cells (24). As shown in the present work, superoxide anions are abundantly produced in C7H2-treated cells.…”

Section: Discussionmentioning

confidence: 55%

β-Actin-binding Complementarity-determining Region 2 of Variable Heavy Chain from Monoclonal Antibody C7 Induces Apoptosis in Several Human Tumor Cells and Is Protective against Metastatic Melanoma

Arruda

Santos

Melo

et al. 2012

Journal of Biological Chemistry

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Background:Immunoglobulin complementarity-determining region (CDR) peptides frequently display antitumor activities. Results: Monoclonal antibody C7 CDR-H2 binds to ␤-actin and induces polymerization, F-actin stabilization, and tumor cell death. Conclusion: Alterations of actin dynamics trigger reactive oxygen species and tumor cell apoptosis. Significance: The in vitro apoptotic effect and in vivo antimetastatic activity of peptide C7H2 makes it a candidate to be developed as an anticancer drug.

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“…As previously described, there may be a crosstalk between both apoptotic pathways mediated by Bid 36, 37. Alternatively, caspase‐8 could be triggered by ROS, mediating the mitochondrial pathway and bypassing CD95/Fas engagement 25, 38, 39. It has also been reported the possibility of an interdependence of caspase‐2, ‐8, ‐9, and ‐3 in an apoptotic process mediated by ROS 17, 40.…”

Section: Discussionmentioning

confidence: 84%

AC‐1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma model

et al. 2016

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Antibody‐derived peptides modulate functions of the immune system and are a source of anti‐infective and antitumor substances. Recent studies have shown that they comprise amino acid sequences of immunoglobulin complementarity‐determining regions, but also fragments of constant regions. VH CDR3 of murine mAb AC‐1001 displays antimetastatic activities using B16F10‐Nex2 murine melanoma cells in a syngeneic model. The peptide was cytotoxic in vitro in murine and human melanoma cells inducing reactive oxygen species (ROS) and apoptosis by the intrinsic pathway. Signs of autophagy were also suggested by the increased expression of LC3/LC3II and Beclin 1 and by ultrastructural evidence. AC‐1001 H3 bound to both G‐ and F‐actin and inhibited tumor cell migration. These results are important evidence of the antitumor activity of Ig CDR‐derived peptides.

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N,N-dimethyl phytosphingosine induces caspase-8-dependent cytochrome c release and apoptosis through ROS generation in human leukemia cells

Cited by 57 publications

References 42 publications

Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

β-Actin-binding Complementarity-determining Region 2 of Variable Heavy Chain from Monoclonal Antibody C7 Induces Apoptosis in Several Human Tumor Cells and Is Protective against Metastatic Melanoma

AC‐1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma model

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