Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Bu, Tongliang; Jia, Yudong; Lin, Jingkai; Mi, Yuling; Zhang, Caiqiao

doi:10.1631/jzus.b1100318

Cited by 16 publications

(13 citation statements)

References 33 publications

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“…Nevertheless, previous data concerning the effects of natural biomolecules on sperm proteins are sparse and primarily focus on peptides playing an important role in the intracellular antioxidant defense mechanisms, such as reduced glutathione (GSH). Our data on the ability of particularly QUE to prevent oxidative damage of proteins are in agreement with Ben Abdallah et al [ 46 ] and Bu et al [ 47 ], who observed that QUE supplementation significantly restored the levels of depleted GSH and protected the activities of intracellular enzymes in testicular germ cells. Nevertheless, further studies on the specific mechanism of action by which natural antioxidants protect either structural or functional proteins from oxidative insults are most needed.…”

Section: Discussionsupporting

confidence: 92%

Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Tvrdá

Debacker

Ďuračka

et al. 2020

Animals

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In this study, we evaluated the impact of 5–50 μM quercetin (QUE) and naringenin (NAR) on extended boar spermatozoa in the BTS (Beltsville Thawing Solution) medium for 72 h. Spermatozoa motion, membrane, acrosome, and DNA integrity were investigated immediately after sample dilution (0 h) as well as after 24 h, 48 h, and 72 h of semen storage. Furthermore, reactive oxygen species (ROS) and superoxide production, as well as the extent of oxidative damage to the sperm proteins and lipids, were assessed to determine the potential of QUE and NAR to prevent a potential loss of sperm vitality due to oxidative stress development. Our results indicate that the most notable parameter influenced by QUE was the mitochondrial activity, which remained significantly higher throughout the experiment (p < 0.001 and p < 0.0001; 10 μM), and which correlated with the most prominent maintenance of sperm motility (p < 0.01, 48 h; p < 0.05, 72 h). A significant membrane stabilization (p < 0.01, 24 h and 48 h; p < 0.0001, 72 h) and prevention of lipid peroxidation (p < 0.05, 24 h and 48 h; p < 0.01, 72 h) was primarily observed following administration of 10 and 25 μM NAR; respectively. Administration of 10 μM QUE led to a significant decrease of superoxide (p < 0.0001, 48 h and 72 h) while the most notable decline of ROS generation was recorded in the case of 10 and 25 μM NAR (p < 0.001). This study may provide new information on the specific mechanisms of action involved in the favorable effects of natural biomolecules on spermatozoa.

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Section: Discussionsupporting

confidence: 92%

Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Tvrdá

Debacker

Ďuračka

et al. 2020

Animals

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“…Yue et al [32] reported that tubular diameter increased significantly in immature rats. Bu et al [34] showed that PNMC at low doses did not affect tubular diameter while high doses of the substance (100 mg/kg PNMC) increased tubular diameter and caused germ cell loss, which was compatible with our findings.…”

Section: Discussionsupporting

confidence: 93%

Sıçan Testisinde 3-Metil-4-Nitrofenol İle Oluşturulan Hasara Karşı Morinda citrifolia (Noni)’nın Koruyucu Etkisi

Yigit¹,

Armutak²,

Esener³

et al. 2016

Kafkas Univ Vet Fak Derg

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In this study, the protective effect of Morinda citrifolia (Noni) against 3-methyl-4-nitrophenol (PNMC) toxicity in rat testes was investigated with an experimental period of five days. Fifty-six adult male Sprague-Dawley rats were allocated into seven experimental groups and a control (n:7). One group received only Noni. Testicular tissue injury of six experimental groups was induced by subcutaneous injection of PNMC at three different doses (1, 10 and 100 mg/kg) and three received Noni treatment (2 ml per rat by gavage). On day six all rats were sacrificed and then blood samples and testis tissues were collected. Serum testosterone, FSH and LH levels were assessed. Testicular tissues were evaluated histomorphometrically in terms of tubular diameter, seminiferous epithelium density, luminal space and interstitial tissue and immunohistochemically labelled with inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) markers to assess oxidative damage. Histomorphometrically, most severe tissue injury was observed in the group of 10 mg/kg PNMC. Tissue injury improved significantly in its corresponding treatment group (with Noni). iNOS and eNOS levels increased in all PNMC groups and decreased with Noni treatments. Noni was most effective in the group of 100mg/kg PNMC in terms of oxidative damage. Serum hormone levels revealed no significant results. In conclusion, Noni reduced PNMC-induced tissue injury in rat testes. Rat, Noni, Testes, iNOS, eNOS Keywords: Sıçan Testisinde 3-Metil-4-Nitrofenol İle Oluşturulan Hasara Karşı Morinda citrifolia (Noni)'nın Koruyucu Etkisi ÖzetBu çalışmada beş günlük deneysel bir periyotla Morinda citrifolia (Noni)'nın sıçan testislerinde 3-metil 4-nitrofenol (PNMC) toksisitesine karşı koruyucu etkisi araştırıldı. Ellialtı adet yetişkin erkek Sprague-Dawley sıçanı eşit sayıda yedi deneysel, bir kontrol grubuna ayrıldı (n:7). Bir gruba sadece Noni uygulandı. Altı deneysel grupta testiste doku hasarı farklı dozlarda (1, 10 and 100 mg/kg) subkutan PNMC enjeksiyonu ile indüklendi ve üç gruba Noni tedavisi uygulandı (her sıçan için 2ml gavaj ile). Altıncı günde tüm sıçanlar sakrifiye edilerek kan ve testis doku örnekleri toplandı. Serum testosteron, FSH ve LH seviyeleri değerlendirildi. Testis dokuları, tubuler çap, seminifer epitel yoğunluğu, lumen aralığı ve interstisyel doku açısından histomorfometrik olarak incelendi ve oksitatif hasarı değerlendirmek üzere indüklenebilir nitrik oksit sentaz (iNOS) ve endotelyal nitrik oksit sentaz (eNOS) belirteçleri ile immunohistokimyasal olarak işaretlendi. Histomorfometrik olarak en şiddetli doku hasarı 10 mg/kg'lık PNMC grubunda gözlendi. Doku hasarı bu gruba karşılık gelen tedavi (Noni ile) grubunda anlamlı ölçüde iyileşti. iNOS ve eNOS düzeyleri tüm PNMC gruplarında yükseldi ve Noni tedavisi ile aynı değerler düşüş gösterdi. Noni'nin oksidatif hasar bakımından en çok 100mg/kg'lık PNMC grubunda etkin olduğu izlendi. Serum hormon değerleri anlamlı sonuçlar vermedi. Sonuç olarak, Noni, sıçan testislerinde PNM...

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“…These results, when taken together, clearly indicated that PP and PNMC disrupted the redox status balance in the host splenocytes/immune cells. This data is consistent with previous studies that established the effect of PNMC on oxidative stress parameters in several models (Mi et al 2009;Bu et al 2012). The data also provide support for the proposal that PP-/PNMCinduced toxicity in splenocytes may be due at least in part to oxidative damage.…”

Section: Discussionsupporting

confidence: 92%

In vitroeffect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production

Yang

Wan

et al. 2016

Journal of Immunotoxicology

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View Crossmark data Citing articles: 3 View citing articles JOURNAL OF IMMUNOTOXICOLOGY, 2016 VOL. 13, NO. 4, 548-556 http://dx.doi.org/10.3109/1547691X.2016 RESEARCH ARTICLEIn vitro effect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production Gasoline exhaust particles (GEP) and diesel exhaust particles (DEP) are considered to be some of the most important air pollutants. Among the many constituents in these pollutant particles, 4-pentylphenol (PP) and 3-methyl-4-nitrophenol (PNMC) are considered important phenolics in GEP and DEP, respectively. The aim of this study was to investigate the effect of in vitro exposure to commercially-supplied PP and PNMC on populations of, and production of interleukin (IL)-2, IL-4 and granzyme-B by, mouse splenic lymphocytes. After in vitro exposure to PP or PNMC for 48 h, splenocyte viability was measured, cell phenotypes, e.g. B-cell (CD19), T-cells (CD3), T-cell subsets (CD4 and CD8), were quantified by flow cytometry and production of IL-2, IL-4 and granzyme-B was assessed via ELISA. The oxidative toxicity of PP and PNMC toward the splenocytes was also evaluated using measures of hydroxyl radical and malondiadehyde production and changes in glutathione peroxidase and superoxide dismutase activities. Results showed that in vitro exposure to PP and PNMC inhibited splenic cell parameters in a dose-related manner. Exposure to PP and PNMC decreased splenic T-lymphocyte populations and splenocyte production of cytokines and granzyme B, as well as induced oxidative stress in the splenocytes. The results also showed that the percentages of CD3 + T-cells overall and of CD4 + and CD8 + T-cells therein, among exposed splenocytes, were reduced; neither compound appeared to affect levels of CD19 + B-cells. Overall, the suppressive effects of PP were stronger than PNMC. The data here provide support for the proposal that PP-/PNMC-induced toxicity in splenocytes may be due at least in part to oxidative damage and that PP and PNMC -as components of GEP and DEP -might significantly impact on splenic T-cell formation/release of cytokines/granzymes in situ.ARTICLE HISTORY

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Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Cited by 16 publications

References 33 publications

Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Sıçan Testisinde 3-Metil-4-Nitrofenol İle Oluşturulan Hasara Karşı Morinda citrifolia (Noni)’nın Koruyucu Etkisi

In vitroeffect of 4-pentylphenol and 3-methyl-4-nitrophenol on murine splenic lymphocyte populations and cytokine/granzyme production

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