Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Tvrdá, Eva; Debacker, Megané; Ďuračka, Michal; Kováč, Ján; Bučko, Ondřej

doi:10.3390/ani10101930

Cited by 26 publications

(31 citation statements)

References 51 publications

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“…This could be easily related to the ameliorative effects of L. sativum extract on glutamate-induced DNA damage, cell viability, and the antioxidant effects or scavenging activity. This is in good agreement with two recent studies which demonstrated that quercetin and nariginin were shown to induce mitochondrial activity, significant membrane stabilization, and prevention of lipid peroxidation of treated cells [ 40 , 41 ]. Table 2 demonstrates the richness of L. sativum with tannins, flavonoids, alkaloids, and anthraquinones as phytochemical with well-documented therapeutic potency.…”

Section: Discussionsupporting

confidence: 92%

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Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells

Al-Dbass

Amina

Musayeib

et al. 2021

Translational Neuroscience

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Glutamate excitotoxicity is considered one of the major causes of retinal ganglion cell death in many retinal diseases. Retinal ganglion cell degeneration causes severe blindness since visual signals from the eye to the brain are conducted only through retinal ganglion cells. Objective: We aimed to explore the potential ameliorative effects of L. sativum against glutamate excitotoxicity-induced retinal ganglion cell damage. Methods: Pure retinal ganglion cells were divided into a control group (untreated); L. sativum-treated groups in which retinal ganglion cells were treated with 5, 10, 50, or 100 µg/mL L. sativum seed extract for 2 h; glutamate-treated groups in which cells were treated with 5, 10, 50, or 100 µM glutamate for 48 h; and L. sativum/glutamate groups [pretreatment with L. sativum for 2 h (50 or 100 µg/mL) before glutamate treatment at 100 µM for 48 h]. Cell damage was assessed by comet assay and cell viability was by MTT test. Results: Tailed DNA, tail length, and tail moment of the 50 and 100 mM glutamate-treated groups were significantly greater than those of the blank control group, while the L. sativum-treated groups demonstrated nonsignificantly different tailed DNA, tail length, and tail moment compared with the blank control group, but significantly lower values compared with the glutamate-treated groups. Conclusion: L. sativum ameliorated the cell viability in retinal ganglion cells after high-concentration glutamate exposure. L. sativum seed extracts were efficient anti-excitotoxic and antioxidant agent that might improve the clinical presentation of many neurological disorders.

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Section: Discussionsupporting

confidence: 92%

“…The antioxidant effects of L. sativum are shown in Table 6 . This could be attributed to the multiple antioxidant compounds recorded in this plant ( Tables 1 and 2 ) which demonstrate high scavenging power of lipid peroxides and ROS [ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells

Al-Dbass

Amina

Musayeib

et al. 2021

Translational Neuroscience

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“…According to Diao et al [ 79 ], 10 μmol/L QUE could significantly improve the motion behavior of spermatozoa collected from leukocytospermic patients. In vitro studies on animal spermatozoa also agree that exposure of male gametes to particularly QUE concentrations ranging between 10 and 25 μmol/L may lead to a higher preservation of motility in bovine [ 80 ] and boar spermatozoa [ 81 , 82 ]. Furthermore, pivotal experiments on cryopreserved boar [ 83 ], stallion [ 84 ], red fowl [ 85 ] and ram [ 86 ] spermatozoa suggest that low QUE concentrations administered to the freezing and thawing medium improve the sperm motility and a subsequent fertilization potential.…”

Section: Quercetinmentioning

confidence: 91%

“…Recent in vitro studies indicate that QUE is a highly efficient biomolecule in preventing the loss of the mitochondrial membrane potential [ 81 ] and a subsequent mitochondrial dysfunction by its ability to accumulate inside the mitochondria [ 90 ] and to control ROS production by its antioxidant activity [ 87 ]. The mitochondrial system is considered to be the primary source of intracellular ROS, whereas QUE may play important roles in the absorption and neutralization of ROS created mainly by the activity of NADPH oxidase and NADH-dependent oxidoreductase, localized in the inner mitochondrial membranes [ 65 , 66 , 91 ].…”

Section: Quercetinmentioning

confidence: 99%

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

et al. 2021

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Emerging evidence from in vivo as well as in vitro studies indicates that natural biomolecules may play important roles in the prevention or management of a wide array of chronic diseases. Furthermore, the use of natural compounds in the treatment of male sub- or infertility has been proposed as a potential alternative to conventional therapeutic options. As such, we aimed to evaluate the effects of selected natural biomolecules on the sperm production, structural integrity, and functional activity. At the same time, we reviewed their possible beneficial or adverse effects on male reproductive health. Using relevant keywords, a literature search was performed to collect currently available information regarding molecular mechanisms by which selected natural biomolecules exhibit their biological effects in the context of male reproductive dysfunction. Evidence gathered from clinical trials, in vitro experiments and in vivo studies suggest that the selected natural compounds affect key targets related to sperm mitochondrial metabolism and motion behavior, oxidative stress, inflammation, DNA integrity and cell death. The majority of reports emphasize on ameliorative, stimulating and protective effects of natural biomolecules on the sperm function. Nevertheless, possible adverse and toxic behavior of natural compounds has been indicated as well, pointing out to a possible dose-dependent impact of natural biomolecules on the sperm survival and functionality. As such, further research leading to a deeper understanding of the beneficial or adverse roles of natural compounds is necessary before these can be employed for the management of male reproductive dysfunction.

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“…Similar to QRC, naringenin (NRG, 2,3-dihydro-5,7-dihydroxy-2-(4-hydroxyphenyl)-4 H -1-benzopyran-4-one) is a natural flavonoid belonging to flavanones, commonly available in tomatoes, bergamot, and citrus fruits that merit further attention [ 21 ]. In particular, recent experiments carried out on boar semen [ 22 ] found that NRG was more effective against lipid peroxidation, while QRC acted as a stronger protective agent against protein oxidation.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Human Sperm Mitochondrial Respiration Efficiency by Plant Polyphenols

et al. 2021

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Plant bioactives, such as polyphenols, can differentially affect (positively or negatively) sperm quality, depending on their concentration. These molecules have been proposed as natural scavengers of reactive oxygen species (ROS) for male infertility treatment. However, few data are available about their effects on the molecular mechanisms related to sperm quality and, in particular, to sperm mitochondrial function. We investigated the effects of quercetin, naringenin, genistein, apigenin, luteolin, and resveratrol at the concentration of 0.1–1000 nM on mitochondrial respiration efficiency. Upon chemical exposure, spermatozoa were swollen in a hypotonic solution and used for polarographic assays of mitochondrial respiration. All tested compounds, except for apigenin, caused a significant increase in the mitochondrial respiration efficiency at the concentration of 0.1 nM, and a significant decrease starting from concentrations of 10 nM. The analysis of oxygen consumption rate in the active and in the resting state of mitochondrial respiration suggested different mechanisms by which the tested compounds modulate mitochondrial function. Therefore, by virtue of their ability to stimulate the respiration active state, quercetin, genistein, and luteolin were found to improve mitochondrial function in asthenozoospermic samples. Our results are relevant to the debate on the promises and perils of natural antioxidants in nutraceutical supplementation.

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Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Cited by 26 publications

References 51 publications

Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells

Lepidium sativum as candidate against excitotoxicity in retinal ganglion cells

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

Modulation of Human Sperm Mitochondrial Respiration Efficiency by Plant Polyphenols

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