N,N-Dimethyl-p-phenylenediamine dihydrochloride-based method for the measurement of plasma oxidative capacity during human aging

Mehdi, Mohammad Murtaza; Rizvi, Syed Ibrahim

doi:10.1016/j.ab.2013.01.032

Cited by 20 publications

(16 citation statements)

References 10 publications

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“…The spectrophotometric DMPD method, described by [171,206,207] Fogliano et al in 1999, is similar to the ABTS method. In the presence of an adequate oxidant solution, the radical cation DMPD…”

Section: Flavonoids -From Biosynthesis To Human Healthmentioning

confidence: 99%

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Antioxidant Capacity of Anthocyanin Pigments

Martín¹,

Kuskoski²,

Navas³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

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Anthocyanins are a family of natural pigments classified into the group of flavonoids, considered to be responsible for the color and taste of many fruits and vegetables, i.e. berries. Anthocyanins are common components of the human diet. Besides their interest as colorant because of their coloring properties, the study of anthocyanin compounds stems from their wide applicability in the prevention and even in the treatment of various human diseases. However, various aspects of the pharmacological roles of anthocyanins remain in the dark, having still several obstacles to the development of robust diets or prescribing lines on consumption of anthocyanins. The chemical structure of anthocyanins determines in large measure its capacity and efficacy as an antioxidant agent. In this study, the following aspects are reviewed: the antioxidant effect of anthocyanin pigments; the oxidative stress, the bioavailability after intake and biological aspects of anthocyanins, the method for measuring the antioxidant activity of anthocyanins, the relationship between structure and activity; and the influence of the anthocyanins in the antioxidant activity of wines. Finally an overview of some potential uses in food industry is attempted mainly focusing in the anthocyanin encapsulation topic. Attention has been paid to the more recent publications in the field.

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Section: Flavonoids -From Biosynthesis To Human Healthmentioning

confidence: 99%

“…DMPD cannot be used with hydrophobic antioxidants, as it is only water soluble [171,206]. DMPD method is not considered suitable for assays of coloured compounds, as interference can occur in the measurements, because they absorb in the same region of the spectrum.…”

Section: Flavonoids -From Biosynthesis To Human Healthmentioning

confidence: 99%

Antioxidant Capacity of Anthocyanin Pigments

Martín¹,

Kuskoski²,

Navas³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

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“…The plasma oxidant capacity in terms of plasma ferric equivalent oxidative potential (PFEOP) was measured according to method of Mehdi and Rizvi [ 22 ]. Briefly 100 mM stock solution of DMPD was prepared by dissolving 209 mg of DMPD in 10 mL of distilled water.…”

Section: Methodsmentioning

confidence: 99%

Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin

Singh

Kesharwani

Misra

et al. 2016

Biochemistry Research International

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Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present throughout all cell types. It transfers electron from intracellular substrates to extracellular acceptors for regulation of redox status. Curcumin, isolated from Curcuma longa, has modulatory effects on cellular physiology due to its membrane interaction ability and antioxidant potential. The present study investigates the effect of curcumin on PMRS activity of erythrocytes isolated from Wistar rats in vitro and in vivo and validated through an in silico docking simulation study using Molegro Virtual Docker (MVD). Effects of curcumin were also evaluated on level of glutathione (GSH) and the oxidant potential of plasma measured in terms of plasma ferric equivalent oxidative potentials (PFEOP). Results show that curcumin significantly (p < 0.01) downregulated the PMRS activity in a dose-dependent manner. Molecular docking results suggest that curcumin interacts with amino acids at the active site cavity of cytochrome b 5 reductase, a key constituent of PMRS. Curcumin also increased the GSH level in erythrocytes and plasma while simultaneously decreasing the oxidant potential (PFEOP) of plasma. Altered PMRS activity and redox status are associated with the pathophysiology of several health complications including aging and diabetes; hence, the above finding may explain part of the role of curcumin in health beneficial effects.

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“…An increased potential for oxidative stress increases the risk of developing age-related pathologies, including cardiovascular disease. Reprinted with permission from Mehdi & Rizvi (2013 69 1. OXIDATIVE STRESS AND AGING this pathway is the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP).…”

Section: The Molecular Basis Of Oxidative Stress As Applied To the Camentioning

confidence: 99%

“…3.3). 7 This increased oxidant potential is indicative of the increasing imbalance between the generation and clearance of ROS throughout the biologic aging process, contributing to the pathogenesis of agerelated cardiovascular disease. Furthermore, as age increases, the antioxidant activity of several antioxidant enzymes in the cardiovascular system decreases (see Fig.…”

Section: Introductionmentioning

confidence: 99%