“…Moreover, adenovirus-mediated knockdown of COP1 could increase ATGL levels and ameliorate steatosis in HFD-fed mice. , Moreover, N-end rule pathway E3 ligases UBR1 and UBR2 modulated ATGL levels in liver. Knock-in mice with N-end rule-resistant mutation of ATGL (F2A) ( Atgl F2A/F2A ) exhibit enhanced lipolysis and were less susceptible to high fat-induced hepatic steatosis and obesity . E3 ligase peroxin2 (PEX2) also ubiquitinates ATGL.…”