N-chlorosuccinimide enhancing the antimicrobial effect of benzalkonium chloride on biofilm <i>Pseudomonas aeruginosa</i> and its interaction mechanisms

Huang, Zaihui; Zheng, Qi; Ding, Xin

doi:10.1080/10934529.2022.2095176

Cited by 2 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The toxicity of aliphatic and aromatic alcohols for P. putida was measured by inhibition of the DHA activity [51]. DHA activity was also used for the evaluation of the potential impact of BACs on the microbial activity of sludge [13,52], as well as the biofilm of P. aeruginosa [53]. Regarding the DHA response to BAC in our study, only P. putida MSCL 650 cultivated in the presence of 50 mg/L BAC at 23 • C showed DHA activity (Figure A3C,E (see Appendix A)).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Biofilm Formation and Bacterial Resistance to Benzalkonium Chloride under Contrasting Cultivation Conditions

et al. 2023

View full text Add to dashboard Cite

Benzalkonium chloride (BAC) is one of the most commonly used quaternary ammonium compounds in the pharmaceutical, cosmetic, and food industries. The aim of our study was to compare the physiological responses of Escherichia coli MSCL 332, Pseudomonas putida MCCL 650, and Staphylococcus epidermidis MSCL 333 on 50 mg/L BAC in rich and poor medium (100% and 5% tryptone soya broth (TSB)) in the temperature range from 8 °C to 37 °C, under static and shaking conditions. A high-throughput, 96-well microplate method was used to compare a broad range of cultivation conditions. The effect of BAC on growth, biofilm formation activity, and dehydrogenase and fluorescein diacetate hydrolysis activity was evaluated. Addition of BAC to 100% TSB inhibited biofilm formation at 37 °C by 2.4, 1.8, and 1.6 times for E. coli, P. putida, and S. epidermidis, respectively. In turn, BAC stimulated biofilm formation in E. coli in 5% TSB at 37 °C and 100% TSB at 8 °C, i.e., 1.4 and 1.3 times, respectively. Statistical optimization of broth composition with emphasis on biofilm formation and further testing under experimental conditions was performed with P. putida.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of Biofilm Formation and Bacterial Resistance to Benzalkonium Chloride under Contrasting Cultivation Conditions

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Liu and co-workers showed that 0.04− 0.07 mmol/L N-chlorosuccinimide pretreatment increased the effectiveness of benzalkonium chloride on biofilm cells by 30%−70% because of increased membrane permeability, oxidative damage, and metabolic disorder. 125 In the study by Laretta Garde and co-workers, combinations of modern commercial QACs [Prontosan (PHMB), octeniline (octenidine dihydrochloride)], ethylene diamine tetra-acetic acid (EDTA), and serine protease (Esperase) were investigated. 126 According to the results, a clear synergistic effect of QACs and EDTA was observed when exposed to biofilms.…”

Section: Qacsmentioning

confidence: 99%

From Antibacterial to Antibiofilm Targeting: An Emerging Paradigm Shift in the Development of Quaternary Ammonium Compounds (QACs)

et al. 2023

View full text Add to dashboard Cite

In a previous development stage, mostly individual antibacterial activity was a target in the optimization of biologically active compounds and antiseptic agents. Although this targeting is still valuable, a new trend has appeared since the discovery of superhigh resistance of bacterial cells upon their aggregation into groups. Indeed, it is now well established that the great majority of pathogenic germs are found in the environment as surface-associated microbial communities called biofilms. The protective properties of biofilms and microbial resistance, even to high concentrations of biocides, cause many chronic infections in medical settings and lead to serious economic losses in various areas. A paradigm shift from individual bacterial targeting to also affecting more complex cellular frameworks is taking place and involves multiple strategies for combating biofilms with compounds that are effective at different stages of microbiome formation. Quaternary ammonium compounds (QACs) play a key role in many of these treatments and prophylactic techniques on the basis of both the use of individual antibacterial agents and combination technologies. In this review, we summarize the literature data on the effectiveness of using commercially available and newly synthesized QACs, as well as synergistic treatment techniques based on them. As an important focus, techniques for developing and applying antimicrobial coatings that prevent the formation of biofilms on various surfaces over time are discussed. The information analyzed in this review will be useful to researchers and engineers working in many fields, including the development of a new generation of applied materials; understanding biofilm surface growth; and conducting research in medical, pharmaceutical, and materials sciences. Although regular studies of antibacterial activity are still widely conducted, a promising new trend is also to evaluate antibiofilm activity in a comprehensive study in order to meet the current requirements for the development of highly needed practical applications.

show abstract

N-chlorosuccinimide enhancing the antimicrobial effect of benzalkonium chloride on biofilm Pseudomonas aeruginosa and its interaction mechanisms

Cited by 2 publications

References 42 publications

Characterization of Biofilm Formation and Bacterial Resistance to Benzalkonium Chloride under Contrasting Cultivation Conditions

Characterization of Biofilm Formation and Bacterial Resistance to Benzalkonium Chloride under Contrasting Cultivation Conditions

From Antibacterial to Antibiofilm Targeting: An Emerging Paradigm Shift in the Development of Quaternary Ammonium Compounds (QACs)

Contact Info

Product

Resources

About