N-acryloxysuccinimide-containing copolymeric microspheres for the immobilization of bimolecules

Yoshida, Minoru; Asano, Masaharu; Yokota, Tsutomu; Chosdu, Rahayu; Kumakura, Minoru

doi:10.1002/pol.1989.140271107

Cited by 16 publications

(7 citation statements)

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“…Particles with amino, hydroxyl, carbonyl, succinimide, benzotriazole, epoxide groups were usually used for this purpose [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Often, the covalent binding of proteins onto a polymer support was accompanied with the undesired (difficult to control) physical adsorption.…”

Section: Introductionmentioning

confidence: 99%

Poly(styrene/acrolein) and poly(styrene/α-tert-butoxy-ω- vinylbenzyl-polyglycidol) microspheres. Similarities and differences

Basiǹska

2002

E-Polymers

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Properties of two types of polystyrene microspheres with polyacrolein and polyglycidol components in their surface layers are compared. Microspheres were prepared in batch radical emulsifier-free emulsion copolymerizations of styrene with acrolein and/or α-tert-butoxy-ω-vinylbenzyl-polyglycidol macromonomer (M n = 2700). Polymerizations were initiated with potassium peroxodisulfate, and the ratio of initial concentrations of styrene and initiator was constant. Number average diameters of poly(styrene/acrolein) (P(S/A)) and of poly(styrene/polyglycidol) (P(S/PGL)) particles were in the range of 200 -650 nm and decreased with increasing concentration of acrolein and/or polyglycidol in the polymerizing mixtures. The diameter polydispersity of synthesized particles (D /D w n ) was usually lower than 1.02. X-ray photoelectron spectroscopy for P(S/A) and P(S/PGL) microspheres showed that surface layers of particles were significantly enriched in polyacrolein or polyglycidol segments, surface fractions of which increased with increasing concentration of the more hydrophilic comonomer in the polymerizing mixture. In the case of P(S/A) particles, the maximal fraction of polyacrolein approached 80 mol-%, whereas for P(S/PGL) particles the maximal surface fraction of PGL was 42 mol-%. Human serum albumin was used as a model protein for studies of attachment onto P(S/A) and P(S/PGL) microspheres. It has been found that for both kinds of particles, the maximal surface concentration of attached (adsorbed and/or covalently immobilized) protein decreased with increasing fraction of hydrophilic component in the particle surface layer (polyacrolein or polyglycidol units). In the case of P(S/A) particles, adsorption always accompanied covalent immobilization of proteins. To the contrary, covalent immobilization of proteins onto the P(S/PGL) microspheres proceeded without adsorption of proteins.

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Section: Introductionmentioning

confidence: 99%

Poly(styrene/acrolein) and poly(styrene/α-tert-butoxy-ω- vinylbenzyl-polyglycidol) microspheres. Similarities and differences

Basiǹska

2002

E-Polymers

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“…Since that time, polymer microspheres have been widely employed in protein and nucleic acid separations, immunoassays and diagnosis, for the immobilization of polysaccharides and enzymes, and in cell labeling and embolization . Depending on the application, polymer microspheres were typically 0.1–100 µm in size and contained various functional groups such as aldehyde, carboxyl, hydroxyl, amino, and oxirane groups . Emulsion polymerization was often used to prepare the polymer particles; however, monodisperse microspheres of desirable size and surface properties can be produced with advantage by dispersion polymerization, which is a one‐step technique that does not require the emulsification of monomers in the reaction medium .…”

Section: Introductionmentioning

confidence: 99%

“…12,13 Depending on the application, polymer microspheres were typically 0.1-100 mm in size and contained various functional groups such as aldehyde, carboxyl, hydroxyl, amino, and oxirane groups. 2,7,14,15 Emulsion polymerization was often used to prepare the polymer particles; however, monodisperse microspheres of desirable size and surface properties can be produced with advantage by dispersion polymerization, which is a one-step technique that does not require the emulsification of monomers in the reaction medium. 15,16 On the contrary, the monomer, stabilizer, and initiator are dissolved in the solvent, from which the polymer precipitates and forms nuclei which continue to grow into the mature polymer particles.…”

mentioning

confidence: 99%

RAFT of sulfobetaine for modifying poly(glycidyl methacrylate) microspheres to reduce nonspecific protein adsorption

Koubková

Macková

Proks

et al. 2015

J. Polym. Sci. Part A: Polym. Chem.

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The minimization of nonspecific protein adsorption is a crucial step in the development of bioseparation processes, immunoassays, and affinity diagnostics. Among the numerous biomaterials, polyzwitterions are known to effectively suppress protein and cell adhesion. This article describes the formation of monodisperse polymer microspheres coated with polysulfobetaine with the aim to limit nonspecific adsorption of bovine serum albumin (BSA) as a model protein. In this process, 2-lm poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization. To render the microspheres hydrophilic and biocompatible, [3-(methacryloylamino)propyl]dimethyl(3-sulfopropyl)ammonium hydroxide (MPDSAH) was grafted from the surface by reversible addition-fragmentation chain transfer (RAFT) polymerization. Elemental analysis of the modified microspheres revealed up to 20 wt % of poly{[3-(methacryloylamino)propyl]dimethyl(3-sulfopropyl)ammonimum hydroxide} (PMPDSAH). The microspheres were characterized in terms of particle size, morphology, and zeta potential. The amount of BSA nonspecifically adsorbed on the PMPDSAH-modified microspheres decreased to half of that captured on the unmodified PGMA microspheres.

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“…There have been several reports about RIGP of polar monomers onto polymer film to obtain hydrophilic property for versatile applications. [7][8][9] For example, an ion-exchange membrane can be used in water desalination, 10 as a carrier for immobilization of medical products, 11 as a separator in alkaline batteries, 12,13 and in other application. 14,15 Enzymes are widely used as biocatalysts in chemical, pharmaceutical and food industrials, and as specific ligands in clinical analysis.…”

Section: Introductionmentioning

confidence: 99%

Radiolytic immobilization of lipase on poly(glycidyl methacrylate)-grafted polyethylene microbeads

et al. 2004

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Poly(glycidyl methacrylate)-grafted polyethylene microbeads (PGPM) presenting epoxy groups were prepared by radiation-induced graft polymerization of glycidyl methacrylate on the polyethylene microbead. The obtained PGPM was characterized by IR spectroscopic, X-ray photoelectrons spectroscopy (XPS), scanning electron microscope (SEM), and thermal analyses. Furthermore, the abundance of epoxy groups on the PGPM was determined by titration and elemental analysis after amination. The epoxy group content was calculated to be in the range 0.29~0.34 mmol/g when using the titration method, but in the range 0.53~0.59 mmol/g when using elemental analysis (EA) after amination. The lipase was immobilized to the epoxy groups of the PGPM under various experimental conditions, including changes to the pH and the epoxy group content. The activity of the lipase-immobilized PGPM was in the range from 160 to 500 unit/mg·min. The activity of the lipase-immobilized PGPM increased upon increasing the epoxy group content. The lipase-immobilized PGPM was characterized additionally by SEM, electron spectroscopy for chemical analysis (ESCA), and EA.

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N-acryloxysuccinimide-containing copolymeric microspheres for the immobilization of bimolecules

Cited by 16 publications

References 1 publication

Poly(styrene/acrolein) and poly(styrene/α-tert-butoxy-ω- vinylbenzyl-polyglycidol) microspheres. Similarities and differences

Poly(styrene/acrolein) and poly(styrene/α-tert-butoxy-ω- vinylbenzyl-polyglycidol) microspheres. Similarities and differences

RAFT of sulfobetaine for modifying poly(glycidyl methacrylate) microspheres to reduce nonspecific protein adsorption

Radiolytic immobilization of lipase on poly(glycidyl methacrylate)-grafted polyethylene microbeads

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