N-acetylglucosamine-6-phosphate deacetylase (NagA) of Listeria monocytogenes EGD, an essential enzyme for the metabolism and recycling of amino sugars

Popowska, Magdalena; Osińska, Magdalena; Rzeczkowska, Magdalena

doi:10.1007/s00203-011-0752-3

Cited by 24 publications

(27 citation statements)

References 57 publications

(78 reference statements)

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“…N-Gluc catalyzes the deacetylation of N-acetylglucosamine-6-phosphate (GlcNAc-6-phosphate) to yield glucosamine-6-phosphate (GlcN-6-P), an intermediate step in the production of uridine diphosphate-GlcNAc (UDP-GlcNAc) [65], [66]. UDP-GlcNAc is a crucial metabolite used in the synthesis of cell walls in bacteria and chitin in yeast and insects, as well as in the GlcNAc moiety of N-linked glycosylation and the glycophosphatidylinositol (GPI) anchor of membrane proteins [65], [67].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Profiling of Diverse Aedes aegypti Strains Reveals Increased Basal-level Immune Activation in Dengue Virus-refractory Populations and Identifies Novel Virus-vector Molecular Interactions

et al. 2013

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Genetic variation among Aedes aegypti populations can greatly influence their vector competence for human pathogens such as the dengue virus (DENV). While intra-species transcriptome differences remain relatively unstudied when compared to coding sequence polymorphisms, they also affect numerous aspects of mosquito biology. Comparative molecular profiling of mosquito strain transcriptomes can therefore provide valuable insight into the regulation of vector competence. We established a panel of A. aegypti strains with varying levels of susceptibility to DENV, comprising both laboratory-maintained strains and field-derived colonies collected from geographically distinct dengue-endemic regions spanning South America, the Caribbean, and Southeast Asia. A comparative genome-wide gene expression microarray-based analysis revealed higher basal levels of numerous immunity-related gene transcripts in DENV-refractory mosquito strains than in susceptible strains, and RNA interference assays further showed different degrees of immune pathway contribution to refractoriness in different strains. By correlating transcript abundance patterns with DENV susceptibility across our panel, we also identified new candidate modulators of DENV infection in the mosquito, and we provide functional evidence for two potential DENV host factors and one potential restriction factor. Our comparative transcriptome dataset thus not only provides valuable information about immune gene regulation and usage in natural refractoriness of mosquito populations to dengue virus but also allows us to identify new molecular interactions between the virus and its mosquito vector.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Profiling of Diverse Aedes aegypti Strains Reveals Increased Basal-level Immune Activation in Dengue Virus-refractory Populations and Identifies Novel Virus-vector Molecular Interactions

et al. 2013

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“…Teichoic acids, along with peptidoglycan, are the main components of the cell wall of Gram-positive bacteria and are thought to control the overall surface charge and affect murein hydrolase activity [17]. L. monocytogenes mutants in genes involved in the synthesis of teichoic acids exhibit decreased integrity of the cell envelope [41], [42]. Furthermore, teichoic acids have been shown to contribute to lysozyme resistance in staphylococci, by preventing the binding of this cell wall hydrolase to its peptidoglycan target [57].…”

Section: Resultsmentioning

confidence: 99%

“…Abnormal cell wall structure and cell morphology have been observed in L. monocytogenes when the content of teichoic acids in the cell wall is reduced [41], [42]. In daptomycin-resistant isolates of S. aureus an increase in the TA content was accompanied by an increase in the thickness of the cell wall [58].…”

Section: Resultsmentioning

confidence: 99%

“…The peptidoglycan of the studied strains was isolated, purified and then digested with the muramidase mutanolysin M1 (Sigma-Aldrich) as described previously [42], except that the purified murein was not N-acetylated before digestion. Soluble muropeptides were reduced by treatment with sodium borohydride and then analyzed by HPLC on a Hypersil octadecylsilane (ODS) reversed-phase column (Teknochroma) according to the method of Glauner [43].…”

Section: Methodsmentioning

confidence: 99%

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Critical Role of a Ferritin-Like Protein in the Control of Listeria monocytogenes Cell Envelope Structure and Stability under β-lactam Pressure

Krawczyk‐Balska

Lipiak

2013

PLoS ONE

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The human pathogen Listeria monocytogenes is susceptible to the β-lactam antibiotics penicillin G and ampicillin, and these are the drugs of choice for the treatment of listerial infections. However, these antibiotics exert only a bacteriostatic effect on this bacterium and consequently, L. monocytogenes is regarded as β-lactam tolerant. It is widely accepted that the phenomenon of bacterial tolerance to β-lactams is due to the lack of adequate autolysin activity, but the mechanisms of L. monocytogenes tolerance to this class of antibiotics are poorly characterized. A ferritin-like protein (Fri) was recently identified as a mediator of β-lactam tolerance in L. monocytogenes, but its function in this process remains unknown. The present study was undertaken to improve our understanding of L. monocytogenes tolerance to β-lactams and to characterize the role of Fri in this phenomenon. A comparative physiological analysis of wild-type L. monocytogenes and a fri deletion mutant provided evidence of a multilevel mechanism controlling autolysin activity in cells grown under β-lactam pressure, which leads to a reduction in the level and/or activity of cell wall-associated autolysins. This is accompanied by increases in the amount of teichoic acids, cell wall thickness and cell envelope integrity of L. monocytogenes grown in the presence of penicillin G, and provides the basis for the innate β-lactam tolerance of this bacterium. Furthermore, this study revealed the inability of the L. monocytogenes Δ fri mutant to deplete autolysins from the cell wall, to adjust the content of teichoic acids and to maintain their D-alanylation at the correct level when treated with penicillin G, thus providing further evidence that Fri is involved in the control of L. monocytogenes cell envelope structure and stability under β-lactam pressure.

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“…monocytogenes B also regulates the transcription of the nagABR operon (13, 72), which encodes two deaminases necessary for N-acetylglucosamine (GlcNAc) degradation (NagA and NagB) (82) and NagR, which functions as a transcriptional inhibitor in the absence of GlcNAc (83). Apart from being a vital part of the bacterial cell wall, monomeric GlcNAc is also a major component of chitin; therefore, GlcNAc is among the most abundant carbon sources in the environment.…”

Section: In Metabolism Of Harmful Components and Utilization Of Diffementioning

confidence: 99%

Resilience in the Face of Uncertainty: Sigma Factor B Fine-Tunes Gene Expression To Support Homeostasis in Gram-Positive Bacteria

Guldimann

Boor

Wiedmann

et al. 2016

Appl Environ Microbiol

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Gram-positive bacteria are ubiquitous and diverse microorganisms that can survive and sometimes even thrive in continuously changing environments. The key to such resilience is the ability of members of a population to respond and adjust to dynamic conditions in the environment. In bacteria, such responses and adjustments are mediated, at least in part, through appropriate changes in the bacterial transcriptome in response to the conditions encountered. Resilience is important for bacterial survival in diverse, complex, and rapidly changing environments and requires coordinated networks that integrate individual, mechanistic responses to environmental cues to enable overall metabolic homeostasis. In many Gram-positive bacteria, a key transcriptional regulator of the response to changing environmental conditions is the alternative sigma factor B . B has been characterized in a subset of Gram-positive bacteria, including the genera Bacillus, Listeria, and Staphylococcus. Recent insight from nextgeneration-sequencing results indicates that B -dependent regulation of gene expression contributes to resilience, i.e., the coordination of complex networks responsive to environmental changes. This review explores contributions of B to resilience in Bacillus, Listeria, and Staphylococcus and illustrates recently described regulatory functions of B .

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N-acetylglucosamine-6-phosphate deacetylase (NagA) of Listeria monocytogenes EGD, an essential enzyme for the metabolism and recycling of amino sugars

Cited by 24 publications

References 57 publications

Transcriptomic Profiling of Diverse Aedes aegypti Strains Reveals Increased Basal-level Immune Activation in Dengue Virus-refractory Populations and Identifies Novel Virus-vector Molecular Interactions

Transcriptomic Profiling of Diverse Aedes aegypti Strains Reveals Increased Basal-level Immune Activation in Dengue Virus-refractory Populations and Identifies Novel Virus-vector Molecular Interactions

Critical Role of a Ferritin-Like Protein in the Control of Listeria monocytogenes Cell Envelope Structure and Stability under β-lactam Pressure

Resilience in the Face of Uncertainty: Sigma Factor B Fine-Tunes Gene Expression To Support Homeostasis in Gram-Positive Bacteria

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