Myringosclerosis Develops within 9 h of Myringotomy

Abstract

The aim of the present experimental study was to elucidate the temporal development of myringosclerosis. Twenty-four Sprague-Dawley rats were myringotomized bilaterally. At 3, 6, 9, 12, 18, 24, 30, 36, 48, 60, 72 and 84 h after the myringotomy, 2 animals at each time were examined otomicroscopically and thereafter sacrificed. The pars flaccida and pars tensa were excised and prepared for light- and electron-microscopic studies. Otomicroscopically, myringosclerosis was visible in the pars tensa 24 h after myringotomy, whereas no sclerotic lesions were noted in the pars flaccida. Histologically, sclerotic lesions were present in the pars tensa and pars flaccida 9 and 12 h, respectively, after myringotomy. The pars flaccida responds promptly with an inflammatory reaction characterized by abundant macrophages. Myringosclerosis develops promptly after myringotomy and its establishment is related to an inflammatory reaction.

Calcium deposition and expression of bone modelling markers in the tympanic membrane following acute otitis media

“…Raustyte et al (+) represents only sporadic or isolated staining; + represents staining in up to approximately 25% of examined tissue; ++ represents staining in up to approximately 50% of examined tissue; +++ represents staining in more than 50% of examined tissue. The microscopic tympanic membrane calcification occurs although no clinical, otomicroscopical signs of sclerosis are evident, which is in agreement with prior investigations [1]. The macrophage may be central in the process and pathogenesis of tympanosclerosis.…”

“…Clinically, myringosclerotic lesions are seen as whitish, bone-like sclerotic plaques in the TM. In accordance with clinical findings, myringosclerosis develops after otitis media (OM) and paracentesis in an experimental setting [1,2]. The pathogenesis of this phenomenon of calcification is still inadequately understood.…”

“…Simple myringotomy induces a wound in the tympanic membrane. The wound healing involves an inflammatory reaction, which leads to sclerosis [1,29,30]. In case of pre-existing inflammation at myringotomy, as seen during AOM, a high number of calcium scavenging macrophages is already present, and this may explain why myringosclerosis is less extensive in case of a concurrent inflammation in an experimental setting [2].…”

A New Trend in the Management of Pediatric Deep Neck Abscess: Achievement of the Medical Treatment Alone

“…Myringosclerosis, a rare and important complication, is a rapidly developing process. Mattsson et al (7) reported that sclerotic changes in the tympanic membrane developed within 9 hours of myringotomy and showed that a severe inflammatory response histologically occurred 12-24 hours after myringotomy in pars flaccida. Tympanosclerosis, which is histologically seen in 80% of cases with myringotomy, can only be seen in 40% of cases with otomicroscopy.…”

“…It is considered a special scar tissue or healed inflammation after recurrent otitis media (ROM). The second speculation is that it is the direct effect of hydrolytic enzymes in serous fluid due to lamina propria (2,(5)(6)(7). Another etiological factor is trauma.…”