Myricitrin Protects Cardiomyocytes from Hypoxia/Reoxygenation Injury: Involvement of Heat Shock Protein 90

Wang, Min; Sun, Guibo; Du, Yuyang; Tian, Yu; Liao, Ping; Liu, Xuesong; Ye, Jingxue

doi:10.3389/fphar.2017.00353

Cited by 33 publications

(23 citation statements)

References 40 publications

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“…Importantly, inducing Hsp70 expression can effectively improve cell survival and attenuate stress damage (Sahin et al 2009;Xu et al 2017a;Xu et al 2017b). Heat stress also induces Hsp90, which can help its client proteins mostly associated with cellular pro-survival/anti-apoptotic signal transduction pathway to maintain their correct molecular structure (Chrisostomos et al 2000;Wang et al 2017). Hsp110 also performs an important protective function by suppressing protein aggregation induced by stress (Yamagishi et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress

Yin

Tang

Sun

et al. 2018

Cell Stress and Chaperones

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Heat stress is exacerbated by global warming and affects human and animal health, leading to heart damage caused by imbalances in reactive oxygen species (ROS) and the antioxidant system, acid-base chemistry, electrolytes and respiratory alkalosis. Vitamin C scavenges excess ROS, and sodium bicarbonate maintains acid-base and electrolyte balance, and alleviates respiratory alkalosis. Herein, we explored the ability of vitamin C alone and in combination with equimolar sodium bicarbonate (Vitamin C-Na) to stimulate endogenous antioxidants and heat shock proteins (HSPs) to relieve heat stress in H9C2 cells. Control, vitamin C (20 μg/ml vitamin C for 16 h) and vitamin C-Na (20 μg/ml vitamin C-Na for 16 h) groups were heat-stressed for 1, 3 or 5 h. Granular and vacuolar degeneration, karyopyknosis and damage to nuclei and mitochondria were clearly reduced in treatment groups, as were apoptosis, lactate dehydrogenase activity and ROS and malondialdehyde levels, while superoxide dismutase activity was increased. Additionally, CRYAB, Hsp27, Hsp60 and Hsp70 mRNA levels were upregulated at 3 h (p < 0.01), and protein levels were increased for CRYAB at 0 h (p < 0.05) and 1 h (p < 0.01), and for Hsp70 at 3 and 5 h (p < 0.01). Thus, pre-treatment with vitamin C or vitamin C-Na might protect H9C2 cells against heat damage by enhancing the antioxidant ability and upregulating CRYAB and Hsp70.

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Section: Discussionmentioning

confidence: 99%

Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress

Yin

Tang

Sun

et al. 2018

Cell Stress and Chaperones

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“…For all experiments, the cells were plated at an appropriate density in accordance with the experimental design and were grown for 24 h to reach 70% to 80% confluence before experimentation. The H/R model was built according to previously published methods [ 19 ]. Briefly, H9c2 cardiomyocytes were cultured in DMEM without glucose under hypoxia for 6 h in an anaerobic glove box (Type C, Coy Laboratory, CA, USA) and then transferred to a regular incubator with the medium replaced by a normal medium to mimic reperfusion.…”

Section: Methodsmentioning

confidence: 99%

Calenduloside E Ameliorates Myocardial Ischemia-Reperfusion Injury through Regulation of AMPK and Mitochondrial OPA1

Wang

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

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Calenduloside E (CE) is a natural triterpenoid saponin isolated from Aralia elata (Miq.) Seem., a well-known traditional Chinese medicine. Our previous studies have shown that CE exerts cardiovascular protective effects both in vivo and in vitro. However, its role in myocardial ischemia/reperfusion injury (MIRI) and the mechanism involved are currently unknown. Mitochondrial dynamics play a key role in MIRI. This study investigated the effects of CE on mitochondrial dynamics and the signaling pathways involved in myocardial ischemia/reperfusion (MI/R). The MI/R rat model and the hypoxia/reoxygenation (H/R) cardiomyocyte model were established in this study. CE exerted significant cardioprotective effects in vivo and in vitro by improving cardiac function, decreasing myocardial infarct size, increasing cardiomyocyte viability, and inhibiting cardiomyocyte apoptosis associated with MI/R. Mechanistically, CE restored mitochondrial homeostasis against MI/R injury through improved mitochondrial ultrastructure, enhanced ATP content and mitochondrial membrane potential, and reduced mitochondrial permeability transition pore (MPTP) opening, while promoting mitochondrial fusion and preventing mitochondrial fission. However, genetic silencing of OPA1 by siRNA abolished the beneficial effects of CE on cardiomyocyte survival and mitochondrial dynamics. Moreover, we demonstrated that CE activated AMP-activated protein kinase (AMPK) and treatment with the AMPK inhibitor, compound C, abolished the protective effects of CE on OPA1 expression and mitochondrial function. Overall, this study demonstrates that CE is effective in mitigating MIRI by modulating AMPK activation-mediated OPA1-related mitochondrial fusion.

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“…For instance, it is able to modulate the expression of adhesion molecules, which is important in the pathogenesis of atherosclerosis [62]. It also protects H9c2 cardiomyocytes against hypoxia/reoxygenation-induced oxidative stress and apoptosis [63]. It is also considered a safe flavonoid for use in food and beverages, without evidence of genotoxicity in animal research [64].…”

Section: Isolated Compounds From Brazilian Medicinal Plants With Diurmentioning

confidence: 99%

Promising Medicinal Plants with Diuretic Potential Used in Brazil: State of the Art, Challenges, and Prospects

et al. 2020

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Medicinal plants are used in traditional medicine to treat a wide range of ailments. The knowledge of them is handed down from generation to generation and is described in several pharmacopoeia and in the general literature. The immense biodiversity of the Brazilian flora, covering about 25% of all plant species worldwide, makes Brazil a huge potential source of medicinal plants. Indeed, many of these plant species are already used in the Brazilian ethnopharmacology for their probable effect to induce diuresis, to reduce fluid retention, and to treat cardiovascular and renal disorders. This review article describes and discusses the main native Brazilian medicinal plants (including some of their isolated compounds) used as diuretics. It also gives a comprehensive analysis of the most relevant scientific studies presented to date, as well as addressing a special topic with future prospects for plant species that have not yet been scientifically studied. In brief, several plants can be indicated for more detailed study, with a view to obtain scientific subsidies for a new and effective diuretic medicine in the future. These include Bauhinia forficata, Leandra dasytricha, and Tropaeolum majus. Other species have reputed medicinal properties but lack experimental assays to demonstrate their pharmacological effects (e.g., Mikania hirsutissima, Phyllanthus niruri, and Tagetes minuta). Several active principles are indicated as responsible for the diuretic effects of the plants studied, with emphasis on phenolic compounds as flavonoids, phenolic acids, and xanthones. These results should encourage more detailed preclinical, clinical, and phytochemical investigations on Brazilian plants in the future.

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Myricitrin Protects Cardiomyocytes from Hypoxia/Reoxygenation Injury: Involvement of Heat Shock Protein 90

Cited by 33 publications

References 40 publications

Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress

Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress

Calenduloside E Ameliorates Myocardial Ischemia-Reperfusion Injury through Regulation of AMPK and Mitochondrial OPA1

Promising Medicinal Plants with Diuretic Potential Used in Brazil: State of the Art, Challenges, and Prospects

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