Myricitrin and bioactive extract ofAlbizia amaraleaves: DNA protection and modulation of fertility and antioxidant-related genes expression

Abstract

Context: Albizia species are reported to exhibit many biological activities including antiovulatory properties in female rats and antispermatogenic and antiandrogenic activities in male rats. Objective: The present study investigates the flavonoids of Albizia amara (Roxb.) B. Boivin (Fabaceae) leaves and evaluates their activity on gene expression of fertility and antioxidant glutathione-S-transferaserelated genes of treated female mice in addition to their effect on DNA damage. Materials and methods: Plant materials were extracted by using 70% methanol for 48 h, the extract was chromatographed on a polyamide 6S column, each isolated compound was purified by using Sephadex LH-20 column; its structure was elucidated by chemical and spectral methods. Both the leaves extract and myricitrin (200, 30 mg/kg bw/d, respectively) were assayed for their effect on DNA damage in female mice after four weeks treatment using Comet assay. Their modulatory activity on gene expression of fertility (aromatase CYP19 and luteinizing hormone LH) and antioxidant glutathione-S-transferase (GST)-related genes of treated female mice were investigated by real-time PCR (qPCR). Results: Quercetin-3-O-gentiobioside, myricitrin, quercetin-3-O-a-rhamnopyranoside, myricetin, quercetin and kaempferol were isolated and identified from the studied taxa. Myricitrin and the extract induced low rate of DNA damage (4.8% and 5%, respectively), compared with the untreated control (4.2%) and significantly down-regulated the expression of CYP19 and LH genes and up-regulated GST gene. Discussion and conclusion: Our results highlight the potential effect of the leaves extract of Albizia amara and myricitrin as fertility-regulating phytoconstituents with ability to protect DNA from damage and cells from oxidative stress. ARTICLE HISTORY

Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse

“…One study revealed severe damage to the seminiferous tubules such as the absence of the germinal epithelium from the wall of seminiferous tubules, reduction in the size of the seminiferous tubules, atrophy of the tubules, expansion of interstitial spaces between seminiferous tubule and vacuolization in the seminiferous tubule in diabetic rats [28]. Concomitant with the present study, it was demonstrated that myricitrin can regulate the fertility along with the ability to protect DNA from cells and damage from oxidative stress as its potential effects [29]. Antioxidant treatment with Nacetyl-L-cysteine has been demonstrated as a testicular apoptotic cell death forbidden by regulation of testicular antioxidant defense under diabetic conditions [5].…”

Flavonoid constituents of Dobera glabra leaves: amelioration impact against CCl 4 -induced changes in the genetic materials in male rats

“…The sequences of specific primer of the genes used are listed in Table 1. The relative quantification of the target (GLAST, GLT-1 and SNAT3) to the reference (b-actin) was determined by using the 2 ÀDDCT method (Kassem et al 2016).…”

Sephadex® LH-20, Isolation, and Purification of Flavonoids from Plant Species: A Comprehensive Review

“…Hydroalcoholic fractions of some species have been previously applied for isolation and purification of quercetin ( 98 ): hydro-methanolic (70%) extracts of leaf and aerial part of Albizia amara [ 83 ] and Allium porrum [ 84 ] eluting via methanol and methanol‒water (6:4), respectively, and hydro-ethanolic (50%) extract of sugarcane top part of Saccharum officinarum (eluent: chloroform‒methanol 1:1) [ 53 ]. Furthermore, this aglycone flavonol has been isolated from stem aqueous extract of Bauhinia strychnifolia using methanol as eluent in SLH gel filtration method [ 85 ].…”