Myrciaria tenella (DC.) O. Berg (Myrtaceae) Leaves as a Source of Antioxidant Compounds

Abstract

Myrciaria species are widely studied to identify their chemical composition and evaluate their biological activity. Since evidence supporting the potential antioxidant and antiproliferative activity of Myrciaria tenella is lacking, the aim of this work was to evaluate these activities in six different leaf extracts: hexane (CHE), chloroform (CCE), ethanolic (CEE), methanolic (CME), aqueous final (CFAE), and only aqueous (CAE). The presence of phenolic compounds, tannin, saponin, and ursolic acid was determined by thin layer chromatography (TLC). CEE, CME, and CFAE showed in vitro antioxidant activity at the initiation, propagation, and termination stages of oxidative damage. Moreover, no toxicity was observed in the 3T3 non-cancerous cell line. On the other hand, all extracts promoted cell death in the tumor cell lines human cervical adenocarcinoma cell line (HeLa) and human stomach gastric adenocarcinoma cell line (AGS). Based on these results, the effect of CEE on the AGS cell line was analyzed using flow cytometry, and necrosis and late apoptosis were observed. Finally, the Caenorhabditis elegans model showed that CEE was able to reduce the basal reactive oxygen species (ROS) level. Ultra-performance liquid chromatography (UPLC) analysis showed rutin as the major compound in CEE. Therefore, Myrciaria tenella fresh leaves may be potential sources of molecules possessing antioxidant and antiproliferative activities.

In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

“…Panax notoginseng ethanol extracts also increased lifespan and protected C. elegans against oxidative stress. Similar results were observed by Ribeiro et al [ 18 ] when using ethanol extract from Myrciaria tenella leaves. Moreover, a correlation between stress resistance and lifespan extension has been attributed to polyphenols [ 13 , 55 ] and saponins [ 56 ].…”

“…Considering that AsRE showed excellent in vitro antioxidant activity, the nematode C. elegans was used to evaluate the in vivo antioxidant potential of AsRE. In addition, after the results obtained in the in vitro tests and knowing that high concentrations are used in tests with these worms due to the presence of a thick cuticle [ 18 ], the concentrations 1.0, 3.0 and 5.0 mg/mL were used in the tests in vivo.…”

“…Based on this rationale, different studies have been carried out aiming to evaluate the intrinsic potential of extracts and biomolecules as potential antioxidants through in vitro spectrophotometric assays and in vivo models such as C . elegans [ 16 , 17 , 18 ].…”

“…The nematode C. elegans is used to investigate the processes involving aging and resistance to oxidative stress, and is preferred because of its short life cycle, rapid reproduction, easy handling and relatively clear mechanism of life extension. C. elegans is the model organism most used in research for these purposes as it is an alternative that demonstrates rapid safety and efficacy in the screening of drugs and biomolecules [ 17 , 18 , 19 ].…”

Atividade Antioxidante De Óleos Essencias Da Família Myrtaceae Pelo Método De Dpph: Uma Revisão De Literatura

“…A família Myrtaceae abrange cerca 132 gêneros e 5.950 espécies, encontradas em regiões tropicais e subtropicais no mundo (Farag et al, 2018). É conhecida pelos seus OEs apresentarem propriedades aromáticas e medicinais, e frequentemente têm sido descritos em várias atividades biológicas (Bida et al, 2019), dentre as quais estão: a ação antibacteriana e anticâncer (Dias et al, 2019, Adnan, 2019; antiparasitário (Gevú et al, 2019); antimutagênico (Bugarin et al, 2014) e antioxidante (Singh et al, 2012;Fidelis, et al 2018;Maggio et al, 2019;Ribeiro et al, 2019).…”