In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

Abstract

Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe2+ and Cu2+), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.

Atividades biológicas de Agave sisalana com ênfase para a ação antimicrobiana: uma revisão da literatura

“…Keriko & Mutua (2008), apontam sua atividade inseticida, Raju e Mehta (2009), seu potencial na quimioprevenção e controle do câncer, Silveira et al, (2009), seu uso como antiparasitária, Dunder et al, (2010), como anti-inflamatório e analgésico. Abreu (2013), por sua vez, descreve os seus efeitos sob a atividade comportamental e neuroquímica, Botura et al, (2013), como ovicida, Zhang et al, (2013), imunomodulador, Vieira et al, (2014), antifúngico, Nunes et al, (2015), larvicida, López-Romero et al, (2018), antibacteriano, De Oliveira et al, (2019, anticâncer e Barreto et al, (2020) como antioxidante.…”

Antioxidant activity, xanthine oxidase inhibition and acute oral toxicity of Dillenia philippinensis Rolfe (Dilleniaceae) leaf extract

“…A volume of 25 μL of 0.01% hydrogen peroxide was added to the reaction mixture and incubated for 60 min at 37°C. The standard utilized in the assay was L-ascorbic acid using a concentration range from 39-1250 µg/mL, and the absorbance was measured at 536 nm using Multiskan Go Spectrophotometer manufactured by Thermo Fisher Scientific, Japan (Barreto et al, 2020). The hydroxyl radical scavenging activity was measured using the following equation [1].…”

Antioxidant Baccharis trimera Leaf Extract Suppresses Lipid Accumulation in C. elegans Dependent on Transcription Factor NHR-49

“…Among these characteristics, the following stand out: small size, simple and transparent body, high reproductive capacity, and low cost; these factors facilitate its easy cultivation in laboratories [ 35 ]. In addition to these factors, C. elegans has become, in recent years, a useful model for the study of fat storage [ 36 , 37 ], mainly due to the conservation of lipid metabolism pathways in mammals [ 38 ], as well as, to evaluate the intrinsic potential of extracts and biomolecules as potential antioxidant agents [ 33 , 39 , 40 ], since they have high homology of their genome with the mammalian genome, in relation to the phase I detoxification system (cytochrome p450), phase II [ 41 ], and phase III [ 42 ], which are important for studies of antioxidant activity.…”