Antioxidant Baccharis trimera Leaf Extract Suppresses Lipid Accumulation in C. elegans Dependent on Transcription Factor NHR-49

Abstract

Obesity is a global public health problem that is associated with oxidative stress. One of the strategies for the treatment of obesity is the use of drugs; however, these are expensive and have numerous side effects. Therefore, the search for new alternatives is necessary. Baccharis trimera is used in Brazilian folk medicine for the treatment of obesity. Here, B. trimera leaf extract (BT) showed antioxidant activity in seven in vitro tests, and it was not toxic to 3T3 murine fibroblasts or Caenorhabditis elegans. Furthermore, BT reduces the intracellular amount of reactive oxygen species and increases C. elegans survival. Moreover, these effects were not dependent on transcription factors. The inhibition of fat accumulation by BT in the C. elegans model was also investigated. BT reduced lipid accumulation in animals fed diets without or with high amount of glucose. Furthermore, it was observed using RNA interference (iRNA) that BT depends on the transcription factor NHR-49 to exert its effect. Phytochemical analysis of BT revealed rutin, hyperoside, and 5-caffeoylquinic acid as the main BT components. Thus, these data demonstrate that BT has antioxidant and anti-obesity effects. However, further studies should be conducted to understand the mechanisms involved in its action.

Chemical Constituents from Leaves of Baccharis sphenophylla (Asteraceae) and Their Antioxidant Effects

“…as a slimming tea [ 4 , 5 , 6 , 7 , 8 ]. Moreover, various biological activities have also been described, including antibacterial, antifungal, antiviral, antiprotozoal, anti-inflammatory, cytotoxic, and antioxidant activities [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. Additionally, the genus is known for its aromatic properties and is widely used in the cosmetic and herbal industries.…”

“…Additionally, the genus is known for its aromatic properties and is widely used in the cosmetic and herbal industries. Previous phytochemical studies of the genus reported several classes of natural products, such as cinnamic acid derivatives [ 7 , 8 , 9 , 10 , 11 ], flavonoids [ 10 , 11 , 12 , 13 , 14 , 15 , 16 ], terpenoids [ 11 , 15 , 16 ], and volatile oils [ 17 , 18 , 19 , 20 , 21 , 22 , 23 ].…”