2016
DOI: 10.5713/ajas.16.0749
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Myotube differentiation in clustered regularly interspaced short palindromic repeat/Cas9-mediated MyoD knockout quail myoblast cells

Abstract: ObjectiveIn the livestock industry, the regulatory mechanisms of muscle proliferation and differentiation can be applied to improve traits such as growth and meat production. We investigated the regulatory pathway of MyoD and its role in muscle differentiation in quail myoblast cells.MethodsThe MyoD gene was mutated by the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 technology and single cell-derived MyoD mutant sublines were identified to investigate the global regulatory mechanism … Show more

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Cited by 14 publications
(17 citation statements)
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“…It is known that Pax7 acts as a transcription factor for the regulation of muscle precursor cell status (Kim, Lee, Park, & Park, ), and MyoD as a myogenic regulatory factor for the induction of myogenic differentiation (Kim et al, ). To investigate the functional effects of AGN extract, undifferentiated pCM cells were treated with AGN extract and examined expression of Pax7 and MyoD.…”
Section: Resultsmentioning
confidence: 99%
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“…It is known that Pax7 acts as a transcription factor for the regulation of muscle precursor cell status (Kim, Lee, Park, & Park, ), and MyoD as a myogenic regulatory factor for the induction of myogenic differentiation (Kim et al, ). To investigate the functional effects of AGN extract, undifferentiated pCM cells were treated with AGN extract and examined expression of Pax7 and MyoD.…”
Section: Resultsmentioning
confidence: 99%
“…In both of the undifferentiated and differentiated stage, qRT‐PCR and Western blotting showed that the transcriptional levels of Pax7 were up‐regulated while MyoD expression levels significantly decreased in AGN‐treated pCM cells (Figures and ). Pax7 is known as a transcription factor that plays a critical role in regulation of muscle precursor cells and so, the overexpression of Pax7 could make the myoblast cells stop the proliferation and re‐enter the quiescent stage of satellite cell stage (Kim et al, ). Jang et al .…”
Section: Discussionmentioning
confidence: 99%
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“…Understanding the genes or genetic markers involved in biological functions and regulatory pathways can help to improve economically important traits in the poultry industry [1][2][3]. Thus, functional genomic studies are powerful and effective for investigating the modulatory mechanisms between cell proliferation and differentiation, in particular in skeletal muscles [4][5][6][7]. Our study was conducted in chicken myoblasts derived from embryonic tissue.…”
Section: Introductionmentioning
confidence: 99%