Muscle differentiation induced by p53 signaling pathway-related genes in myostatin-knockout quail myoblasts

Park, Jeong-Woong; Lee, Jeong Hyo; Han, Ji Seon; Shin, Seung Pyo; Park, Tae Sub

doi:10.1007/s11033-020-05935-0

Cited by 12 publications

(7 citation statements)

References 37 publications

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“…The molecular mechanism of MSTN regulation in chicken muscle is not yet fully understood but understanding the potential regulatory mechanism of MSTN is very important in poultry meat production. Previous studies have used transcriptome analysis to explore the transcriptome profile of MSTN knockout (KO) muscle cells of mice and quails to understand the possible molecular mechanism, but few have explored the transcriptome profile of MSTN KO in chicken muscle cells [ 18 , 19 ]. Our aim in the present study was therefore to knock out MSTN in chicken muscle cells to allow a comparison of the transcriptome profiles of MSTN KO cells and WT cells.…”

Section: Discussionmentioning

confidence: 99%

“…Researchers have used CRISPR/Cas9 technology to knock out the MSTN gene in mouse myoblasts (C2C12 cells) and performed RNA-Seq and miRNA-Seq to explore the molecular mechanism of MSTN regulation of muscle cell proliferation [ 18 ]. Other studies have used CRISPR/Cas9 technology to generate MSTN knockout (KO) avian (quail) myoblasts (QM7) to examine the MSTN regulatory networks through RNA-Seq [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts

Zhou

Han

et al. 2021

Genes

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In mammals, Myostatin (MSTN) is a known negative regulator of muscle growth and development, but its role in birds is poorly understood. To investigate the molecular mechanism of MSTN on muscle growth and development in chickens, we knocked out MSTN in chicken fetal myoblasts (CFMs) and sequenced the mRNA transcriptomes. The amplicon sequencing results show that the editing efficiency of the cells was 76%. The transcriptomic results showed that 296 differentially expressed genes were generated after down-regulation of MSTN, including angiotensin I converting enzyme (ACE), extracellular fatty acid-binding protein (EXFABP) and troponin T1, slow skeletal type (TNNT1). These genes are closely associated with myoblast differentiation, muscle growth and energy metabolism. Subsequent enrichment analysis showed that DEGs of CFMs were related to MAPK, P13K/AKT, and STAT3 signaling pathways. The MAPK and P13K/AKT signaling pathways are two of the three known signaling pathways involved in the biological effects of MSTN in mammals, and the STAT3 pathway is also significantly enriched in MSTN knock out chicken leg muscles. The results of this study will help to understand the possible molecular mechanism of MSTN regulating the early differentiation of CFMs and lay a foundation for further research on the molecular mechanism of MSTN involvement in muscle growth and development.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts

Zhou

Han

et al. 2021

Genes

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“…Moreover, inhibition of the MSTN gene expression by shRNA promoted proliferation of the duck embryonic myoblasts [ 25 ]. Also, knockout and overexpression of the MSTN gene in the quail myoblast cell line resulted in an increase and decrease of myotube formation, respectively [ 26 , 27 ], indicating regulation of myogenesis by the MSTN gene.…”

Section: Myogenic Function Of the Mstn Gene In Pou...mentioning

confidence: 99%

Myostatin gene role in regulating traits of poultry species for potential industrial applications

Lee,

Kim,

Lee

2024

J Animal Sci Biotechnol

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The myostatin (MSTN) gene is considered a potential genetic marker to improve economically important traits in livestock, since the discovery of its function using the MSTN knockout mice. The anti-myogenic function of the MSTN gene was further demonstrated in farm animal species with natural or induced mutations. In poultry species, myogenesis in cell culture was regulated by modulation of the MSTN gene. Also, different expression levels of the MSTN gene in poultry models with different muscle mass have been reported, indicating the conserved myogenic function of the MSTN gene between mammalian and avian species. Recent advances of CRISPR/Cas9-mediated genome editing techniques have led to development of genome-edited poultry species targeting the MSTN gene to clearly demonstrate its anti-myogenic function and further investigate other potential functions in poultry species. This review summarizes research conducted to understand the function of the MSTN gene in various poultry models from cells to whole organisms. Furthermore, the genome-edited poultry models targeting the MSTN gene are reviewed to integrate diverse effects of the MSTN gene on different traits of poultry species.

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“…Increasingly, on metric of the value of an animal model will be the extent to which specific tools are available for affecting specific manipulations of its genome. Particularly exciting is the actual editing of the avian genome using lentiviral, adenoviral, electroporation, and CRISPR/Cas9 gene editing, which will further enhance the growing list of genetically modified birds (McGrew et al, 2004 ; Kobayashi et al, 2015 ; Véron et al, 2015 ; Sid and Schusser, 2018 ; Chojnacka-Puchta and Sawicka, 2020 ; Lee et al, 2020 ; Park J. S. et al, 2020 ; Park J. W. et al, 2020 ; Khwatenge and Nahashon, 2021 ). These technologies are allowing phenotype modification meeting both enhanced production goals in domesticated birds and creating modified birds for basic exploration of biological systems during development and in adulthood (Chojnacka-Puchta and Sawicka, 2020 ).…”

Section: Alternative Avian Models For Research In Developmentmentioning

confidence: 99%

“…These technologies are allowing phenotype modification meeting both enhanced production goals in domesticated birds and creating modified birds for basic exploration of biological systems during development and in adulthood (Chojnacka-Puchta and Sawicka, 2020 ). Doubtlessly, the chicken genome has to date been the major focus of these technologies, but primordial germ cell cultures are available for the duck, goose, turkey quail, and pheasant, and experimentally altered phenotypes in these species are emerging from the use of gene editing (Bo et al, 2016 ; Chen et al, 2019 ; Lee et al, 2019 ; Park J. W. et al, 2020 ).…”

Section: Alternative Avian Models For Research In Developmentmentioning

confidence: 99%

Beyond the Chicken: Alternative Avian Models for Developmental Physiological Research

Flores-Santin

Burggren

2021

Front. Physiol.

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Biomedical research focusing on physiological, morphological, behavioral, and other aspects of development has long depended upon the chicken (Gallus gallus domesticus) as a key animal model that is presumed to be typical of birds and generally applicable to mammals. Yet, the modern chicken in its many forms is the result of artificial selection more intense than almost any other domesticated animal. A consequence of great variation in genotype and phenotype is that some breeds have inherent aberrant physiological and morphological traits that may show up relatively early in development (e.g., hypertension, hyperglycemia, and limb defects in the broiler chickens). While such traits can be useful as models of specific diseases, this high degree of specialization can color general experimental results and affect their translational value. Against this background, in this review we first consider the characteristics that make an animal model attractive for developmental research (e.g., accessibility, ease of rearing, size, fecundity, development rates, genetic variation, etc.). We then explore opportunities presented by the embryo to adult continuum of alternative bird models, including quail, ratites, songbirds, birds of prey, and corvids. We conclude by indicating that expanding developmental studies beyond the chicken model to include additional avian groups will both validate the chicken model as well as potentially identify even more suitable avian models for answering questions applicable to both basic biology and the human condition.

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Muscle differentiation induced by p53 signaling pathway-related genes in myostatin-knockout quail myoblasts

Cited by 12 publications

References 37 publications

Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts

Transcriptomic Analysis of MSTN Knockout in the Early Differentiation of Chicken Fetal Myoblasts

Myostatin gene role in regulating traits of poultry species for potential industrial applications

Beyond the Chicken: Alternative Avian Models for Developmental Physiological Research

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