Myostatin inhibits porcine intramuscular preadipocyte differentiation in vitro

Sun, Wenxing; Dodson, Michael V.; Jiang, Zhihua; Yu, Shijie; Chu, Weiwei; Chen, J.

doi:10.1016/j.domaniend.2015.10.005

Cited by 22 publications

(17 citation statements)

References 32 publications

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“…4). We previously found that MSTN could inhibit the differentiation of pre-adipocytes 26 . Therefore, we presumed that MSTN contributes to the MCM inhibiting effect on intramuscular adipocytes.…”

Section: Resultsmentioning

confidence: 97%

“…Some cytokines including IL-1α, IL-2, IL-4, TNFα, INFα and NF-κB could impact the GR expression in many cell types 48–50 . Besides, the other myokines including IL-6, IL-15, TNFα, Irisin, and MSTN which could inducing thermogenesis or inhibit adipogenesis might have the ability to regulate expression of GR 26, 51–54 . Our previous study found that myokines MSTN inhibited the differentiation of porcine adipocytes 26 .…”

Section: Discussionmentioning

confidence: 99%

“…Besides, the other myokines including IL-6, IL-15, TNFα, Irisin, and MSTN which could inducing thermogenesis or inhibit adipogenesis might have the ability to regulate expression of GR 26, 51–54 . Our previous study found that myokines MSTN inhibited the differentiation of porcine adipocytes 26 . Hence, we detected the concentration of MSTN in muscle, and the results showed that concentration of MSTN in muscle MCM was extremely high.…”

Section: Discussionmentioning

confidence: 99%

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Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue

Chu

Wei

Han

et al. 2017

Sci Rep

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Intramuscular adipose is conducive to good pork quality, whereas subcutaneous adipose is considered as waste in pig production. So uncovering the regulation differences between these two adiposes is helpful to tissue-specific control of fat deposition. In this study, we found the sensitivity to glucocorticoids (GCs) was lower in intramuscular adipocytes (IMA) compared with subcutaneous adipocytes (SA). Comparison of glucocorticoid receptor (GR) revealed that IMA had lower GR level which contributed to its reduced GCs sensitivity. Higher methylation levels of GR promotor 1-C and 1-H were detected in IMA compared with SA. GR expression decrease was also found in adipocytes when treated with muscle conditioned medium (MCM) in vitro, which resulted in significant inhibition of adipocytes proliferation and differentiation. Since abundant myostatin (MSTN) was detected in MCM by ELISA assay, we further investigated the effect of this myokine on adipocytes. MSTN treatment suppressed adipocytes GR expression, cell proliferation and differentiation, which mimicked the effects of MCM. The methylation levels of GR promotor 1-C and 1-H were also elevated after MSTN treatment. Our study reveals the role of GR in muscle fiber inhibition on intramuscular adipocytes, and identifies myostatin as a muscle-derived modulator for adipose GR level.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue

Chu

Wei

Han

et al. 2017

Sci Rep

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“…In addition, another adipogenesis transcription factor, C/EBPɑ , and lipid metabolism-related genes such as glycerol-3-phosphate dehydrogenase ( GPDH ), diacylglycerol O-acyltransferase ( DGAT ), acyl-CoA synthetase long-chain family member1 ( ACS1 ), adipose triglyceride lipase ( ATGL ), and hormone-sensitive lipase ( HSL ) are inhibited by MSTN in 3T3-L1 preadipocyte adipose differentiation [25]. Moreover, the adipogenesis of primary preadipocytes isolated from bovine, porcine fat tissue and intramuscular preadipocytes isolated from porcine longissimus dorsi muscles is also inhibited by treatment with adipogenesis medium plus MSTN [6, 26, 27]. …”

Section: Differentiation Is Inhibited By Mstn In Preadipocytesmentioning

confidence: 99%

“…Myostatin (MSTN), a negative regulator of skeletal muscle growth, can be detected in not only muscle tissues but also adipose tissues [3]. Evidence has now been obtained demonstrating that MSTN could regulate the adipogenesis of mesenchymal stem cells in the determination and differentiation phases [4–6]. In this review, we mainly summarize the structure, tissue distribution, and signal transduction of MSTN and explore the role of MSTN in the adipogenesis of preadipocytes, multipotent stem cells and transgenic animals in mammals.…”

Section: Introductionmentioning

confidence: 99%

The function of myostatin in the regulation of fat mass in mammals

et al. 2017

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Myostatin (MSTN), also referred to as growth and differentiation factor-8, is a protein secreted in muscle tissues. Researchers believe that its primary function is in negatively regulating muscle because a mutation in its coding region can lead to the famous double muscle trait in cattle. Muscle and adipose tissue develop from the same mesenchymal stem cells, and researchers have found that MSTN is expressed in fat tissues and plays a key role in adipogenesis. Interestingly, MSTN can exert a dual function, either inhibiting or promoting adipogenesis, according to the situation. Due to its potential function in controlling body fat mass, MSTN has attracted the interest of researchers. In this review, we explore its function in regulating adipogenesis in mammals, including preadipocytes, multipotent stem cells and fat mass.

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Resveratrol Inhibits Human Visceral Preadipocyte Proliferation and Differentiation in vitro

Sun

Han

et al. 2019

Lipids

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Visceral obesity is a high‐risk factor for diabetes and metabolic syndrome. Resveratrol, a natural polyphenolic compound, has been reported to inhibit preadipocyte differentiation. However, the effect of resveratrol on human visceral preadipocyte (HPA‐v) differentiation remains largely unknown. LIM domain only 3 (LMO3) promotes human preadipocyte differentiation by enhancing peroxisome proliferator‐activated receptor γ (PPARγ) transcriptional activity, which is the master regulator of adipogenesis. The purpose of our study was to determine the effect of resveratrol (0–50 μM) on HPA‐v proliferation and differentiation, and the role of LMO3 in resveratrol‐mediated regulation of HPA‐v differentiation. Resveratrol inhibited HPA‐v proliferation and differentiation in a dose‐dependent manner, and significantly decreased the mRNA expression levels of PPARG, CCAAT/enhancer‐binding protein α (CEBPA), fatty acid‐binding protein 4 (FABP4), acetyl‐CoA carboxylase (ACC), and fatty acid synthase (FAS) (p < 0.05) at 10, 20, and 50 μM. The mRNA and protein levels of LMO3 were significantly reduced by ≥20 μM resveratrol (p < 0.05), and overexpression of LMO3 partially attenuated resveratrol‐induced reduction of HPA‐v differentiation by enhancing the PPARG transcriptional activity. Together, our study suggested that resveratrol reduced HPA‐v proliferation and differentiation, as well as LMO3, which was partially responsible for the reduction of resveratrol‐mediated adipocyte differentiation.

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Myostatin inhibits porcine intramuscular preadipocyte differentiation in vitro

Cited by 22 publications

References 32 publications

Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue

Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue

The function of myostatin in the regulation of fat mass in mammals

Resveratrol Inhibits Human Visceral Preadipocyte Proliferation and Differentiation in vitro

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