Myosin light chain 2 isoforms in gilthead sea bream (Sparus aurata L.): Molecular growth markers at early stages

Moderate and sustained exercise modulates muscle proteolytic and myogenic markers in gilthead sea bream (Sparus aurata)

“…Very recently, this molecule has been related to the intensive stratified formation of new fibers during muscle growth in larvae of gilthead sea bream (38). Moreover, in concordance with previous studies, it has been suggested that MLC2A is more involved in larval growth (mainly hyperplasic growth) whereas MLC2B is more related to the hypertrophic growth that takes places in the adult stages (39,67). Similarly, Silva et al ( 89) also suggested previously that MLC could be a good marker of hyperplasia in blackspot sea bream.…”

Muscle growth mechanisms in response to isoenergetic changes in dietary non-protein energy source at low and high protein levels in juvenile rainbow trout

“…Fmhc, which gene expression did not correlate (r= 0.23; P=0.18) with individual muscle area, also does not appear here as a valid molecular marker of white muscle growth, in line with other results in trout juveniles (Alami-Durante et al, 2010b, 2018. As opposed, the correlation between fmlc2 gene expression and individual trout length (r=0.39; P=0.02) and weight (r=0.42; P=0.01) and between fmlc2 gene expression and individual white muscle total cross-sectional area (r=0.33; close to significance, P=0.05) indicates that fmlc2 gene expression might be not only a good molecular marker of individual juvenile growth, as previously seen in gilthead sea bream juveniles fed with commercial diets (Georgiou et al, 2014) , but also of individual white muscle growth. Further studies in juvenile fish under different nutritional conditions are needed to specify this finding.…”

Transient up- and down-regulation of expression of myosin light chain 2 and myostatin mRNA mark the changes from stratified hyperplasia to muscle fiber hypertrophy in larvae of gilthead sea bream (Sparus aurata L.)

“…In the present study, a significant increase in mlc2b expression marked the onset of hypertrophy (between 25 and 35 dph) and mosaic hyperplasia (between 45 and 60 dph). In addition, another recent study in gilthead sea bream showed that mlc2b expression continued increasing in the post-larval stages (55-117dph), whereas mlc2a expression dropped to low levels (Georgiou et al 2014). Thus, MLC2B becomes the predominant isoform in adult muscle fibers in gilthead sea bream and its expression is significantly correlated to length growth (Georgiou et al 2014).…”

“…In addition, another recent study in gilthead sea bream showed that mlc2b expression continued increasing in the post-larval stages (55-117dph), whereas mlc2a expression dropped to low levels (Georgiou et al 2014). Thus, MLC2B becomes the predominant isoform in adult muscle fibers in gilthead sea bream and its expression is significantly correlated to length growth (Georgiou et al 2014). An early preliminary study on the developmental expression of mlc1 and mlc2 in gilthead sea bream by in situ hybridization showed that mlc2 expression marked the germinal zones of skeletal muscle and the newly formed white fibers, whereas in juveniles, its expression was restricted to myogenic cells interspersed in the fast white muscle (Moutou et al 2005).…”