MyoD, Myogenin, and Desmin-nls-lacZ Transgene Emphasize the Distinct Patterns of Satellite Cell Activation in Growth and Regeneration

Creuzet, Sophie; Lescaudron, Laurent; Li, Zhenlin; Fontaine-Pérus, Josiane

doi:10.1006/excr.1998.4100

Cited by 61 publications

(46 citation statements)

References 63 publications

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“…5c,f). Pax7 is expressed in quiescent and activated SCs, and MyoD is expressed in SCs that are activated during muscle regeneration 10,35,36 . At 1 day after CTX injection, MyoD þ cells were more numerous in TG mice (Fig.…”

Section: Resultsmentioning

confidence: 99%

MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

Zhai

et al. 2015

Nat Commun

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Skeletal muscle stem cells, called satellite cells, are a quiescent heterogeneous population. Their heterogeneity is influenced by Pax7, a well-defined transcriptional regulator of satellite cell functions that defines two subpopulations: Pax7 Hi and Pax7 Lo . However, the mechanisms by which these subpopulations are established and maintained during myogenesis are not completely understood. Here we show that miR-431, which is predominantly expressed in the skeletal muscle, mediates satellite cell heterogeneity by fine-tuning Pax7 levels during muscle development and regeneration. In miR-431 transgenic mice, the Pax7 Lo subpopulation is enriched, enhances myogenic differentiation and accelerates muscle regeneration. Notably, miR-431 attenuates the muscular dystrophic phenotype in mdx mice and may be a potential therapeutic target in muscular diseases. miR-431 transgenic mice are a unique genetic model for investigating the cellular features and biological functions of Pax7 Lo satellite cells during muscle development and regeneration.

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Section: Resultsmentioning

confidence: 99%

MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

Zhai

et al. 2015

Nat Commun

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“…In other models, using crushing, 27 freezing, 9 or chemically induced injuries to skeletal muscle 12 to study satellite cell activation, proliferation and regeneration, two response of satellite cells to activation have been described. These are based on the integrity of the basal lamina.…”

Section: Discussionmentioning

confidence: 99%

Mobilisation of satellite cells following ischaemia and reperfusion in primate skeletal muscle

Gregory

Mars

2004

SA J. Sports Med.

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Objective. To describe the morphological and morphometric features of activated skeletal muscle satellite cells in primates, using an ischaemic reperfusion model. Setting. The study was undertaken at the Biomedical Resource Centre and the Electron Microscopy Unit of the University of KwaZulu-Natal. Interventions. Eight vervet monkeys were anaesthetised and subjected to 3 hours of tourniquet-induced lower limb ischaemia. Open muscle biopsies were taken from tibialis anterior muscle immediately after tourniquet release and 12, 24, 36 and 48 hours after tourniquet release. Control biopsies were taken from the opposite limb. Main outcome measures. Description of the morphological and morphometric changes in satellite cells after activation, as seen on transmission electron microscopy. Results. Two distinct patterns of satellite cell activation are described. In group 1, the cytoplasm of the satellite cell expands around the myocyte and the gap between the satellite cell and the myocyte appears to break down, or in group 2, the novel observation of the satellite cell breaking away from the myofibre and becoming a myocyte totally encased in its own basal lamina. The satellite cells of group 1 were significantly longer than the group 2 cells (p = 0.018) and this was associated with a significant reduction in the percentage of nuclear to cell area (p = 0.011). Conclusions. Tourniquet-induced ischaemic reperfusion injury is shown to result in two distinct patterns of satellite cell activation which may represent different functions or subsets of satellite cells.

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“…The relevant markers of proliferating satellite cells, which are silent in quiescent satellite cells, are desmin, Myf5, MyoD, and PCNA [86,105,106] (Figure 4). In particular, a Myf5 promoter activity has been demonstrated in resident satellite cells using the knock-in Myf5nLacZ pos [94,107,108].…”

Section: The Phase Of Regeneration Remodeling and Maturationmentioning

confidence: 99%

The Basis of Muscle Regeneration

Musarò

2014

Advances in Biology

117

138

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Muscle regeneration recapitulates many aspects of embryonic myogenesis and is an important homeostatic process of the adult skeletal muscle, which, after development, retains the capacity to regenerate in response to appropriate stimuli, activating the muscle compartment of stem cells, namely, satellite cells, as well as other precursor cells. Moreover, significant evidence suggests that while stem cells represent an important determinant for tissue regeneration, a "qualified" environment is necessary to guarantee and achieve functional results. It is therefore plausible that the loss of control over these cell fate decisions could lead to a pathological transdifferentiation, leading to pathologic defects in the regenerative process. This review provides an overview about the general aspects of muscle development and discusses the cellular and molecular aspects that characterize the five interrelated and time-dependent phases of muscle regeneration, namely, degeneration, inflammation, regeneration, remodeling, and maturation/functional repair.

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MyoD, Myogenin, and Desmin-nls-lacZ Transgene Emphasize the Distinct Patterns of Satellite Cell Activation in Growth and Regeneration

Cited by 61 publications

References 63 publications

MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

Mobilisation of satellite cells following ischaemia and reperfusion in primate skeletal muscle

The Basis of Muscle Regeneration

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