myo-Inositol Metabolism in the Neonatal and Developing Rat Fed a myo-Inositol-free Diet

Abstract

Neonatal rats of the Holtzman strain, 6 days of age, were fed a myo-inositol restricted liquid formula by gastric intubation for 10 days, after which they were fed a purified myo-inositol-free diet until they were 72 days old. No differences in weight gain were observed between myo-inositol/100 ml of formula or 150 mg myo-inositol/100 g diet. Most tissues examined from rats fed the myo-inositol deprived formula and diet had lower free myo-inositol levels than the controls with the exception of the liver. Despite reduced free and lipid-bound myo-inositol in the liver, there was no evidence of fatty liver in the young rats at any age. The cerebrum and cerebellum of myo-inositol deprived rats had normal myelination and mitochondriogenesis as judged by the levels of 2',3'-cyclic nucleotide-3'-phosphohydrolase (EC 3.1.4.1) and fumarase (EC 4.2.1.2) activity, respectively.

Longitudinal 1H MRS of rat forebrain from infancy to adulthood reveals adolescence as a distinctive phase of neurometabolite development

“…, in a report on developing mice conducted at 9.4 T, observed no change in T 1 relaxation in the thalamus as a function of maturation. Following correction for T 1 relaxation effects, our data fit within the range of metabolite concentrations found in literature reports of both in vivo and extracted rat brain neurochemical concentrations measured on or near the developmental ages investigated in this study, as shown in Table .…”

Human breast milk sugars and polyols over the first 10 puerperium days

“…myo ‐Inositol requirements for human keratinocytes are markedly higher than for any other cell type (Gordon et al, ). Rat pups fed with a myo ‐inositol restricted diet in the early neonatal period develop cataract (Burton et al, ).…”

Inositol Phosphatase in Developing Rat Duodenum, Jejunum and Ileum

“…A very high rate of turnover, with a very low concentration of Pis in the intestinal mucosa [20], raises the question whether they might have a role in regulating the absorption of nutrients or cell proliferation in the intes tine. The lipid-bound inositol concentration and Pi-specific phospholipase-C activity (the main enzyme of Pi-turnover cycle) in the small intestine are rapidly increased during the neonatal period of development [21,22]. Recently, we demonstrated the presence of a specific inositol phosphatase in the small in testine [23].…”