Myelin basic protein specific T-helper cells induce experimental anterior uveitis

Adamus, Grażyna; Amundson, Drake; Vainiene, M.; Ariail, K.; Machnicki, Michał; Weinberg, Andrew D.; Offner, H.

doi:10.1002/(sici)1097-4547(19960615)44:6<513::aid-jnr1>3.0.co;2-e

Cited by 40 publications

(6 citation statements)

References 18 publications

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“…The forebrain is attacked only in the most severe cases and in the late stages of the disease (Lassmann et al, 1988). In Lewis rats with MBP‐induced EAE, an anterior uveitis which was delayed compared to the EAE course was reported by Verhagen et al (1994), and by Adamus et al (1996). In our relapsing EAE model, we observed such an anterior uveitis which was also delayed and the rats still displayed severe clinical symptoms 50 days after EAE induction, a long time after complete recovery of EAE.…”

Section: Discussionmentioning

confidence: 64%

“…After EAE induction, retinopathy and optic neuritis were described in rhesus monkeys (von Sallmann et al, 1967; Hayreh et al, 1981; Hayreh, 1981) and optic neuritis was reported in guinea pigs (Rao et al, 1977). Recently, several authors have examined the ophtalmologic manifestations of EAE in Lewis rats and all described the induction of anterior uveitis (Shikishima et al, 1993; Verhagen et al, 1994; Adamus et al, 1996). More recently, Hu et al (1998a,b) investigated the cellular responses in the optic nerve and retina of Lewis rats with MBP‐induced EAE.…”

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confidence: 99%

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Distribution in ocular structures and optic pathways of immunocompetent and glial cells in an experimental allergic encephalomyelitis (EAE) relapsing model

Villarroya

Klein

Thillaye-Goldenberg

et al. 2001

J of Neuroscience Research

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Relapsing experimental allergic encephalomyelitis (EAE) was induced in DA rats and the ocular pathologic events were examined at the various phases of the illness. About 80% of EAE rats presented anterior uveitis (AU), even after complete EAE recovery. We studied the phenotype and localization of immunocompetent cells, the major histocompatibility complex (MHC) class I and II antigen expression, as well as the chemokine monocyte chemoattractant protein-1 (MCP-1) appearance. In control animals, there were many glial fibrillary acidic protein (GFAP)(+) cells and OX42(+) cells in the ciliary body, retina, optic nerve and chiasma. Except in retina, we observed constitutive MHC class I and II expression. During the EAE acute phase, there was up-regulation of MHC class II and GFAP antigens in iris, ciliary body, limbus, and optic pathways. MHC class I and ED2 antigens were expressed in meninges and in the prechiasmatic cisterna, by cells which could have a role in immune surveillance. MCP-1 mRNA was highly expressed in optic pathways during the acute phase and the protein was expressed by astrocytes, macrophages, and lymphocytes. During the relapsing phase, MCP-1 was weakly expressed to disappear almost completely during the final recovery phase. The expression of MHC class II on astrocytes was increased during the relapsing and final recovery phase in which the inflammatory lesions persisted. These findings suggest that ocular areas and optic pathways, mainly optic chiasma, are important targets in the relapsing EAE.

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Section: Discussionmentioning

confidence: 64%

mentioning

confidence: 99%

Distribution in ocular structures and optic pathways of immunocompetent and glial cells in an experimental allergic encephalomyelitis (EAE) relapsing model

Villarroya

Klein

Thillaye-Goldenberg

et al. 2001

J of Neuroscience Research

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“…20 21 This suggests shared antigenic determinants between neurological and ophthalmological manifestations with similar pathomechanisms. 21 Treatment with IFN-b reduced ocular inflammation in this model. 22 This beneficial effect may be useful, especially in patients with partial resistance to systemic corticosteroids or immunosuppressive drugs.…”

Section: Discussionmentioning

confidence: 80%

Interferon as a treatment for uveitis associated with multiple sclerosis

Becker

2005

British Journal of Ophthalmology

111

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“…Several antigens, including endotoxin, MAA and myelin basic protein (MBP), have been used to induce AAU in animals [1, 4, 16]. These animal models are valuable to provide molecular insights into immunological mechanisms for the pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Identification of Hnrph3 as an autoantigen for acute anterior uveitis

Kim

Caberoy

Alvarado

et al. 2011

Clinical Immunology

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Acute anterior uveitis (AAU) is the most common form of autoimmune uveitis in the eye with few known autoantigens. Identification of autoantigens will improve our understanding of the molecular mechanisms and capability for disease diagnosis. Phage display is a powerful technology for autoantigen identification. However, because of uncontrollable reading frames, phage display with conventional cDNA libraries identifies high percentage of non-open reading frames (non-ORFs) with minimal implications for autoantigen identification. We recently developed ORF phage display technology with minimal reading frame problem. Herein we used ORF phage display to identify 18 patient-specific clones, including 16 ORFs encoding endogenous proteins as candidate autoantigens for AAU. One of the identified antigens was heterogeneous nuclear ribonucleoprotein H3 (Hnrph3) that was further characterized for AAU relevance and independently verified by Western blot. These results demonstrate that ORF phage display is a valuable approach for identification of unknown autoantigens.

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Myelin basic protein specific T-helper cells induce experimental anterior uveitis

Cited by 40 publications

References 18 publications

Distribution in ocular structures and optic pathways of immunocompetent and glial cells in an experimental allergic encephalomyelitis (EAE) relapsing model

Distribution in ocular structures and optic pathways of immunocompetent and glial cells in an experimental allergic encephalomyelitis (EAE) relapsing model

Interferon as a treatment for uveitis associated with multiple sclerosis

Identification of Hnrph3 as an autoantigen for acute anterior uveitis

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