Identification of Hnrph3 as an autoantigen for acute anterior uveitis

Kim, Youngbae; Caberoy, Nora B.; Alvarado, Gabriela; Davis, Janet L.; Feuer, William J.; Li, Wei

doi:10.1016/j.clim.2010.09.008

Cited by 17 publications

(15 citation statements)

References 28 publications

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“…Such pre-panning ORF selection minimizes the interference of non-ORF clones and improves the efficiency of ORF phage selection. During multi-round phage selection, however, non-ORF clones displaying unnatural short peptides with high activities may re-emerge but can be conveniently re-eliminated by post-panning ORF selection [24]. Enriched phages after the post-panning selection should have a high percentage of ORF clones that encode real endogenous proteins with specific binding or functional activities.…”

Section: Description Of Methodsmentioning

confidence: 99%

“…Our experience indicated that stringent post-panning ORF selection is important to ORF phage display, particularly with antibody bait [24]. If necessary, multi-round post-panning ORF selection may be performed to ensure a high percentage of ORF clones.…”

Section: Description Of Methodsmentioning

confidence: 99%

“…The interpretation of autoantibody binding to these mimotopes is problematic. We demonstrated the application of ORF phage display to identify real autoantigens for autoimmune uveitis, an autoimmune disease in the eye, and independently validated the identified autoantigens by Western blot using purified recombinant autoantigens [24]. These results suggested that ORF phage display can improve our ability to efficiently identify real autoantigens as disease biomarkers.…”

Section: Application Of Orf Phage Displaymentioning

confidence: 99%

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ORF phage display to identify cellular proteins with different functions

2012

Methods

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Open reading frame (ORF) phage display is a new branch of phage display aimed at improving its efficiency to identify cellular proteins with specific binding or functional activities. Despite the success of phage display with antibody libraries and random peptide libraries, phage display with cDNA libraries of cellular proteins identifies a high percentage of non-ORF clones encoding unnatural short peptides with minimal biological implications. This is mainly because of the uncontrollable reading frames of cellular proteins in conventional cDNA libraries. ORF phage display solves this problem by eliminating non-ORF clones to generate ORF cDNA libraries. Here I summarize the procedures of ORF phage display, discuss the factors influencing its efficiency, present examples of its versatile applications, and highlight evidence of its capability of identifying biologically relevant cellular proteins. ORF phage display coupled with different selection strategies is capable of delineating diverse functions of cellular proteins with unique advantages.

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Section: Description Of Methodsmentioning

confidence: 99%

Section: Description Of Methodsmentioning

confidence: 99%

Section: Application Of Orf Phage Displaymentioning

confidence: 99%

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ORF phage display to identify cellular proteins with different functions

2012

Methods

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“…With the aim of identifying a preferential autoantigen, a recent study involving 31 patients with acute anterior uveitis (24 with HLA-B27) and 13 healthy subjects used purified patients' IgG on a phage amplification system and further demonstrated its preferential binding to maltose-binding protein-Hnrph3 fusion protein (MBP-Hnrph3). The phage display method used in this study seems to be a valuable approach for identification of novel autoantigens like type 1 collagen α2 antigen [46].…”

Section: Identification and Localization Of The Autoantigen(s)mentioning

confidence: 99%

Cutting-Edge Issues in Autoimmune Uveitis

Levy

Andrade²,

Foeldvari

2011

Clinic Rev Allerg Immunol

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Autoimmune uveitis (AIU) is among the leading causes of preventable blindness. It can be isolated, precede, or appear in the course of a systemic autoimmune inflammatory disease. When suspected, AIU should be promptly referred to an ophthalmologist for proper anatomic classification and local treatment. In recurrent and chronic forms, systemic treatment should be started, usually with corticosteroids and immunesuppressors. In cases of lack of efficacy or intolerance, biologic agents such as monoclonal antibodies anti-TNF (infliximab and adalimumab) and others (abatacept and tocilizumab) are being used. The clinical diseases associated to AIU and the experimental models have helped in the understanding of the pathogenic mechanism. The treatment schemes have improved, and recent advances in basic knowledge are leading to even more effective targeted therapies.

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“…It has typically been used for antigen discovery [71]. For example, T7 cDNA display of sea snake venom gland mRNA identified rabbit anti-sea snake venom IgGs as well as new toxins [72].…”

Section: Proteomic Phage Displaymentioning

confidence: 99%

Interaction Analysis through Proteomic Phage Display

Sundell

Ivarsson

2014

BioMed Research International

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Phage display is a powerful technique for profiling specificities of peptide binding domains. The method is suited for the identification of high-affinity ligands with inhibitor potential when using highly diverse combinatorial peptide phage libraries. Such experiments further provide consensus motifs for genome-wide scanning of ligands of potential biological relevance. A complementary but considerably less explored approach is to display expression products of genomic DNA, cDNA, open reading frames (ORFs), or oligonucleotide libraries designed to encode defined regions of a target proteome on phage particles. One of the main applications of such proteomic libraries has been the elucidation of antibody epitopes. This review is focused on the use of proteomic phage display to uncover protein-protein interactions of potential relevance for cellular function. The method is particularly suited for the discovery of interactions between peptide binding domains and their targets. We discuss the largely unexplored potential of this method in the discovery of domain-motif interactions of potential biological relevance.

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Identification of Hnrph3 as an autoantigen for acute anterior uveitis

Cited by 17 publications

References 28 publications

ORF phage display to identify cellular proteins with different functions

ORF phage display to identify cellular proteins with different functions

Cutting-Edge Issues in Autoimmune Uveitis

Interaction Analysis through Proteomic Phage Display

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