Myelin‐Associated Glycoprotein Shares an Antigenic Determinant with a Glycoprotein of Human Melanoma Cells

Noronha, Antonio; Harper, John R.; Ilyas, Amjad A.; Reisfeld, Ralph A.; Quarles, Richard H.

doi:10.1111/j.1471-4159.1986.tb00795.x

Cited by 32 publications

(9 citation statements)

References 56 publications

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“…Immunoblot data on SCLC cells identify two groups of HNK-1-reactive plasma membrane glycoproteins of 80,000 and 130,000 mol wt (33). SCLC cells also release a 100,000-mol wt glycoprotein reactive with HNK-1 into the spent medium of cultured cells (34). A secreted form of NCAM has also been demonstrated in neural tissues (35).…”

Section: Resultsmentioning

confidence: 99%

An adherent subline of a unique small-cell lung cancer cell line downregulates antigens of the neural cell adhesion molecule

1991

Lung Cancer

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Small-cell lung cancer (SCLC) lines are distinguished from non-small-cell lung cancer (NSCLC) lines by their growth in floating aggregates, in contrast to the adherent monolayers formed by NSCLC cells in culture. Of 50 well-characterized SCLC lines recently described by the National Cancer Institute (NCI)-Navy Medical Oncology Branch, only four variant cell lines (SCLC-v) grew as adherent monolayers. One line, NCI-H446, was unique in growing long-term with coexisting floating and surface adherent subpopulations. We have physically segregated these two populations over many passages in vitro to enrich for relatively pure cultures of floating and adherent cells. No differences in c-myc expression, keratin pattern, or cytogenetic appearance were found between the adherent and floating sublines. However, expression of the neuroendocrine marker neuron-specific enolase in the floating cells was three times that found in the adherent cells. The floating subline also had much greater surface expression of neuroendocrine tumor antigens detected by monoclonal antibodies UJ13A and HNK-1, which have been recently shown to detect the neural cell adhesion molecule (NCAM) on SCLC cells. Two other adherent SCLC-v lines were also found to be unreactive with UJ13A and HNK-1, generalizing the association between NCAM expression and the growth of most SCLC cultures as floating aggregates. In conclusion, we have an interesting model to study expression of NCAM as related to the adhesive properties of SCLC cells. (J. Clin. Invest. 1990Invest. . 86:1848Invest. -1854 Key words: lung cancer * neural cell adhesion molecule * neuron-specific enolase Introduction A striking difference between small-cell lung cancer (SCLC)'

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Section: Resultsmentioning

confidence: 99%

An adherent subline of a unique small-cell lung cancer cell line downregulates antigens of the neural cell adhesion molecule

1991

Lung Cancer

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“…However, anti-MAG antibodies have been reported to stain a variety ofother cell types (11)(12)(13)(14)(15), including neurons (16,17). Using antibodies generated against synthetic peptides derived from the 1B236 cDNA sequence, we have also detected 1B236/MAG immunoreactivity within subpopulations of neurons in the adult rat CNS (7,18).…”

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confidence: 86%

Cellular localization of 1B236/myelin-associated glycoprotein mRNA during rat brain development.

Higgins

Schmale

Bloom

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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The protein encoded by the rat brain cDNA 1B236 has been shown to be identical to myelin-associated glycoprotein (MAG). In this report we describe the cellular distribution of 1B236/MAG mRNA transcripts in rat brain by using in situ hybridization. At postnatal day 20, large numbers of 1B236/MAG mRNA-containing oligodendrocytes are concentrated in myelinated fiber tracts and throughout gray matter regions. The presence of high levels of 1B236/MAG mRNA within oligodendrocytes at postnatal day 20 is consistent with the proposed role of MAG in formation of the myelin sheath during development. In the adult brain, our results suggest that not only is 1B236/MAG mRNA expressed at reduced levels within oligodendrocytes but also 1B236/MAG or a lB236/MAG-like mRNA is present within neurons. This localization is consistent with the results of previous immunocytochemical studies using antibodies against the 1B236 protein. The apparent developmental profile of 1B236/MAG mRNA with different cell-type-specific patterns of expression suggests that oligodendrocytes and neurons employ different mechanisms for regulating the same gene. Thus, different cell types may use a similar cell adhesion molecule both during myelinogenesis and in the mature nervous system. Myelin-associated glycoprotein (MAG), a constituent of myelin in both peripheral and central nervous systems (CNS), appears to act as a cell adhesion molecule between glia and neurons (1, 2) and may be involved in formation of the periaxonal space (3). Molecular cloning studies (4-6) have shown that MAG is identical to the rat brain protein 1B236, previously defined by characterization of "brainspecific" mRNAs (7). The single 1B236/MAG gene gives rise to several mRNAs by alternative RNA splicing (4); the omission or inclusion ofexon 12 ofthe gene results in mRNAs encoding polypeptides with different carboxyl-terminal sequences and molecular masses of72 and 67 kDa, respectively (4, 5). During development, 1B236/MAG accumulates in the rat CNS until approximately postnatal day (PD) 20, by which time myelination has largely been completed (1). Similarly, the steady-state level of 1B236 mRNA increases until PD 15-25 and drops in the adult to 20% ofthe maximal levels attained during development (8). This developmental change in 1B236/MAG abundance appears to be accompanied by a transition in expression of the alternatively spliced mRNAs and their products: the 72-kDa form is expressed during postnatal development and myelinogenesis, whereas the 67-kDa form predominates in the adult (4, 9).Immunocytochemistry has been used to demonstrate the presence of MAG within oligodendrocytes in both developing and adult CNS (3, 10), consistent with its proposed role in formation of the myelin sheath. However, anti-MAG antibodies have been reported to stain a variety ofother cell types (11-15), including neurons (16, 17). Using antibodies generated against synthetic peptides derived from the 1B236 cDNA sequence, we have also detected 1B236/MAG immunoreactivity within subpopulations o...

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“…Though the coexistence of these two diseases in the same patient could be merely coincidental, the proposed autoimmune nature of both diseases might suggest a relationship between them. Melanocytes and Schwann cells are derived from the neural crest, share potential surface antigens including MAG [14] and gangliosides (GM2, GM3, GD3, GD2) [15] and thus may share common antigenic components with peripheral nerve. Although we did not detect anti-MAG or anti-GM1 antibodies, the possibility of antibodies directed against a common antigen is intriguing and shared immunoreactivity may have accounted for the association between leukoderma and chronic inflammatory demyelinating polyradiculoneuropathy in our patient.…”

Section: Discussionmentioning

confidence: 99%

Leukoderma and chronic inflammatory demyelinating polyradiculoneuropathy in an adolescent with graft-versus-host disease

Okan¹,

Erbey²,

Durmus³

et al. 2015

MMJ

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A 15-year-old Iraqi male patient with acute lymphoblastic leukemia received an allogeneic bone marrow transplant from his brother. On the 18th day after the transplant, the patient developed grade II acute graft-versus-host disease, and treatment included methylprednisolone. On day 140, the patient was diagnosed with leukoderma. On day 150, the patient was admitted to hospital because of numbness and muscle weakness in the extremities and a disturbance of the gait. Neurologic examination showed muscle weakness in the upper and lower extremities and there were no deep tendon reflexes. Nerve conduction studies showed reduced conduction speeds of the motor nerves with demyelinating features. The patient was diagnosed as having a chronic inflammatory demyelinating polyradiculoneuropathy (CIDB) based on clinical and electrophysiological findings. Treatment included methylprednisolone and intravenous immunoglobulin. The neurologic function improved but the skin lesions persisted. This case shows that graft-versus-host disease can act as a triggering factor for the appearance of autoimmune diseases.

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Myelin‐Associated Glycoprotein Shares an Antigenic Determinant with a Glycoprotein of Human Melanoma Cells

Cited by 32 publications

References 56 publications

An adherent subline of a unique small-cell lung cancer cell line downregulates antigens of the neural cell adhesion molecule

An adherent subline of a unique small-cell lung cancer cell line downregulates antigens of the neural cell adhesion molecule

Cellular localization of 1B236/myelin-associated glycoprotein mRNA during rat brain development.

Leukoderma and chronic inflammatory demyelinating polyradiculoneuropathy in an adolescent with graft-versus-host disease

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