Mycovirus effect on the endophytic establishment of the entomopathogenic fungus Tolypocladium cylindrosporum in tomato and bean plants

Asensio, Noemí Herrero; Márquez, M. Salud Sánchez; Zabalgogeazcoa, Íñigo

doi:10.1007/s10526-012-9476-9

Cited by 15 publications

(7 citation statements)

References 34 publications

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“…Some types of mycovirus have been shown to be detrimental to the host and reduce fungal growth (Van Diepeningen et al, 2006). Autoclaving the media would destroy any mycoviruses but they would still be present in the simply filtered medium, and their presence may have reduced endophyte emergence (Herrero Asensio et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Media Manipulations and the Culture of Beneficial Fungal Root Endophytes

Murphy¹,

Batke²,

Doohan³

et al. 2015

IJB

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Fungal endophytes have great potential as biocontrol and biofertilisation agents for food crops, and it is important to understand and optimise the cultural conditions for endophyte emergence and growth. A range of commercial culture media were tested for their effect on endophyte recovery from the roots of their host (Hordeum murinum), and for their subsequent growth and sporulation. Furthermore, a medium composed of sterile or filtered whole plant extract (WPE) was tested at various dilutions both alone and in combination with one of the commercial media. It was found that there were significant differences between the media in endophyte recovery, endophyte mycelial growth and time to sporulation. A significantly greater number of different endophytes were recovered from roots on the malt extract plus WPE medium than any of the other media tested here. Sabouraud medium gave the greatest mean radial growth of endophyte fungal mycelia after 35 days. The cultures growing on the WPE media took over five times longer to sporulate than all other media. Results indicate that different media are suitable either for the initial recovery and isolation of fungal root endophytes or for increasing fungal biomass and inducing earlier sporulation. The experimental endophytes used here have previously been shown to have biocontrol and biofertilisation benefits for barley cultivars, so our findings have important implications for the development and commercialisation of endophyte inocula.

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Section: Discussionmentioning

confidence: 99%

Media Manipulations and the Culture of Beneficial Fungal Root Endophytes

Murphy¹,

Batke²,

Doohan³

et al. 2015

IJB

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“…Whether certain strains of entomopathogens might be better endophytes than others or have different rates of survival inside plants has been questioned (53). Some evidence indicates that mycoviruses might affect the endophytic capability of fungal strains; in a study of virus-infected and virus-free strains of the entomopathogen T. cylindrosporum inoculated into tomato and bean leaves, the presence of the mycovirus TcV1 affected the performance of isogenic strains in the different host plants (18). A situation like this in B. bassiana could favor the maintenance of some particular viruses in strains that become endophytes of some particular hosts.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Diversity of Viruses in the Entomopathogenic Fungus Beauveria bassiana

Herrero

Dueñas

Quesada‐Moraga

et al. 2012

Appl Environ Microbiol

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bViruses have been discovered in numerous fungal species, but unlike most known animal or plant viruses, they are rarely associated with deleterious effects on their hosts. The knowledge about viruses among entomopathogenic fungi is very limited, although their existence is suspected because of the presence of virus-like double-stranded RNA (dsRNA) in isolates of several species. Beauveria bassiana is one of the most-studied species of entomopathogenic fungi; it has a cosmopolitan distribution and is used as a biological control agent against invertebrates in agriculture. We analyzed a collection of 73 isolates obtained at different locations and from different habitats in Spain and Portugal, searching for dsRNA elements indicative of viral infections. The results revealed that the prevalence of viral infections is high; 54.8% of the isolates contained dsRNA elements with viral characteristics. The dsRNA electropherotypes of infected isolates indicated that virus diversity was high in the collection analyzed and that mixed virus infections occurred in fungal isolates. However, a hybridization experiment indicated that dsRNA bands that are similar in size do not always have similar sequences. Particular virus species or dsRNA profiles were not associated with locations or types of habitats, probably because of the ubiquity and efficient dispersion of this fungus as an airborne species. The sequence of one of the most common dsRNA elements corresponded to the 5.2-kbp genome of a previously undescribed member of the Totiviridae family, termed B. bassiana RNA virus 1 (BbRV1).

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“…There is currently no definitive proof about whether fungus-virus interactions help plant hosts survive in extreme environments, with most previous studies focusing on whether endophytes-both infected and non-infected-can confer heat tolerance to plant hosts [33,69]. For example, the fungus Curvularia protuberia, when infected by a mycovirus, helped panic grass (Dichanthelium lanuginosum) tolerate excessive heat, and researchers intend to investigate whether this strategy can be extended to tomato plants [70]. Viruses have been shown to alter the transcriptome, small RNAome, proteome, metabolome, lipidome, and epigenome of the host.…”

Section: Biological and Ecological Effects Of Mycoviruses In Endophytmentioning

confidence: 99%

Viruses of endophytic and pathogenic forest fungi

Siddique

2020

Virus Genes

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Mycoviruses, just as the fungal endophytes they infect, are ubiquitous biological entities on Earth. Mycoviruses constitute a diverse group of viruses, and metagenomic approaches have-through recent discoveries of been mycoviruses-only recently began to provide evidence of this astonishing diversity. The current review presents (1) various mycoviruses which infect fungal endophytes and forest pathogens, (2) their presumed origins and interactions with fungi, plants and the environment, (3) high-throughput sequencing techniques that can be used to explore the horizontal gene transfer of mycoviruses, and (4) how the hypo-and hypervirulence induced by mycoviral infection is relevant to the biological control of pathogenic fungi.

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Mycovirus effect on the endophytic establishment of the entomopathogenic fungus Tolypocladium cylindrosporum in tomato and bean plants

Cited by 15 publications

References 34 publications

Media Manipulations and the Culture of Beneficial Fungal Root Endophytes

Media Manipulations and the Culture of Beneficial Fungal Root Endophytes

Prevalence and Diversity of Viruses in the Entomopathogenic Fungus Beauveria bassiana

Viruses of endophytic and pathogenic forest fungi

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