Mycoremediation of manganese and phenanthrene by Pleurotus eryngii mycelium enhanced by Tween 80 and saponin

Abstract

Bioremediation of areas co-contaminated with metals and polycyclic aromatic hydrocarbons (PAHs) by mushrooms has attracted considerable attention in recent years. In this study, Pleurotus eryngii was introduced for the removal of Mn and phenanthrene (Phe) from potato liquid medium (PDL) simultaneously. Effects of Tween 80 and saponin on P. eryngii growth together with Mn uptake as well as Phe removal were investigated. Although pollutants had a negative effect on mycelial morphology and growth, P. eryngii could still tolerate and remove Mn and Phe. Tween 80 increased removal of Mn and Phe through increase of P. eryngii growth, Phe solubility, pollutants bioavailability, and specific surface area of mycelium pellets, moreover, the activities of manganese peroxidase (MnP) and laccase, which played an important role on PAHs biodegradation. The maximal removal of Mn and Phe was achieved (92.17 and 93.85 % after 15 days incubation, respectively) with 0.6 g L(-1) Tween 80. Treatments with saponin markedly inhibited P. eryngii growth (50.17-66.32 % lower relative to control) due to its fungistatic activity. Nevertheless, saponin could slightly enhance Phe removal through increasing solubility of Phe, and Phe removal rate varied from 80.53 to 87.06 % in saponin treatments. Joint stress of Mn and Phe induced a strong antioxidative response, and superoxide dismutase (SOD) activity decreased in surfactants-treated mycelium compared with control. Generally, Tween 80 was more suitable for strengthening mycoremediation by P. eryngii than saponin, and could be a promising alternative for the remediation of heavy metals and PAHs co-contaminated sites by mushrooms.

Potential microbial drivers of biodegradation of polycyclic aromatic hydrocarbons in crude oil sludge using a composting technique

“…There also is insufficient information to indicate the contribution of the surfactants to improved bioavailability, which, as reported by Bezza and Chirwa, does not necessarily enhance the process of biodegradation of aromatic PAHs . Although the addition of surfactants had no apparent effect on degradation efficiency, their presence enhanced fungal growth . Enhanced fungal growth upon addition of surfactants signifies that the added surfactants were not toxic to indigenous microbial entities in the various treatments.…”

“…73 Although the addition of surfactants had no apparent effect on degradation efficiency, their presence enhanced fungal growth. 74 Enhanced fungal growth upon addition of surfactants signifies that the added surfactants were not toxic to indigenous microbial entities in the various treatments. This gives credence to previous findings that the toxicity of anionic surfactant SDS to microorganisms is not significant considering the biodegradability of SDS.…”

Degradation of polycyclic aromatic hydrocarbons (phenanthrene and pyrene) by the ligninolytic fungi Ganoderma lucidum isolated from the hardwood stump

“…S133 (Hadibarata and Tachibana 2010), G. lucidum BCRC 36021 (Ting et al 2011), Pleurotus ostreatus sp. ATCC38540 (Tirado-Torres et al 2016), Pleurotus eryngii (Wu et al 2016). Ting et al (2011) observed that G. lucidum BCRC 36021 degraded 98.70% of phenanthrene in basal medium at 30 °C but in this study, phenanthrene degradation was investigated in mineral salt broth, in our knowledge, no one study has been found on the degradation of phenanthrene by G. lucidum strain CCG1 in mineral salt broth.…”

“…Thus, it is necessary to remediate the PAH compound from the environment. The United States Environmental Protection Agency (USEPA) has been listed 16 PAHs as priority pollutants, including phenanthrene and pyrene (Jin et al 2007;Puglisi et al 2007;Wu et al 2016). Phenanthrene and pyrene are considered as model compounds for the PAH degradation study because they were found most abundantly in the PAH-polluted environment (Bezalel et al 1996;Makkar and Rockne 2003;Mrozik and Piotrowska-Seget 2010).…”

Novel self‐immobilized biomass mixture based on mycelium pellets for wastewater treatment: A review

“…Thus, pure pellets are preferred over other materials for wastewater treatment. Several studies have investigated the removal of metals by pure pellets from wastewater, such as Mn, Phe (Wu, Xu, Ding, Li, & Xu, ), Cr (Xu et al., ), and Se (Espinosa‐Ortiz, Rene, Hullebusch, & Lens, ), and found that the pure pellets can perform well within the pH range of 2–7 and the temperature range of 25–35°C.…”