Degradation of polycyclic aromatic hydrocarbons (phenanthrene and pyrene) by the ligninolytic fungi Ganoderma lucidum isolated from the hardwood stump

Abstract

Background: Due to progress in science and technology, several harmful polycyclic aromatic hydrocarbons are synthesized and released into the environment. In the present investigation, a phenanthrene-and pyrene-degrading white rot fungi Ganoderma lucidum strain CCG1 was isolated from the Janjgir Champa district of Chhattisgarh, India, and then the degradation of phenanthrene and pyrene was estimated by high-performance liquid chromatography.Results: It was found that G. lucidum able to degrade 99.65% of 20 mg/L of phenanthrene and 99.58% of pyrene in mineral salt broth after 30th day of incubation at 27 °C. G. lucidum produced significant amounts (p < 0.0001) of ligninolytic enzymes (Laccase, lignin peroxidase and manganese peroxidase) in the phenanthrene-and pyrenecontaining mineral salt broth. G. lucidum produced maximum 10,788.00 U/L laccase, 3283.00 U/L Lignin peroxidase and 47,444.00 U/L Manganese peroxidase enzymes in the presence of phenanthrene and produced maximum 10,166.00 U/L laccase, 3613.00 U/L lignin peroxidase and 50,977.00 U/L manganese peroxidase enzymes in the presence of pyrene. Therefore, G. lucidum will be a potent phenanthrene and pyrene degrader from the environment.

Biodegradation and detoxification of phenanthrene in in vitro and in vivo conditions by a newly isolated ligninolytic fungus Coriolopsis byrsina strain APC5 and characterization of their metabolites for environmental safety

“…Various microbes such as bacterial species, Pseudomonas aeruginosa PFL-P1 (Mahto and Das, 2020), Paracoccus aminovorans HPD-2 (Teng et al, 2010), Burkholderia cepacia (Chen et al, 2013), Roseobacter clade (Zhou et al, 2020) Sphingomonas sp. GY2B (Liu et al, 2017) and fungal species, Trametes hirsuta D7 (Hidayat and Yanto, 2018), Ganoderma lucidum (Agrawal et al, 2018), Pleurotus ostreatus (Pozdnyakova et al, 2018), Leucoagaricus gongylophorus (Ike et al, 2019), and Armillaria sp. FO22 (Hadibarata and Kristanti, 2012) have been reported for the remediation of PAHs contaminated soil, however as compared with bacteria, fungi is a promising alternative for the remediation of PAHs.…”

Biodegradation and detoxification of phenanthrene in in-vitro and in-vivo conditions by a newly isolated ligninolytic fungus Coriolopsis byrsina strain APC5 and characterization of their metabolites for environmental safety

“…Various microbes such as bacterial species, Pseudomonas aeruginosa PFL-P1 (Mahto and Das, 2020), Paracoccus aminovorans HPD-2 (Teng et al, 2010), Burkholderia cepacia (Chen et al, 2013), Roseobacter clade (Zhou et al, 2020) Sphingomonas sp. GY2B (Liu et al, 2017) and fungal species, Trametes hirsuta D7 (Hidayat and Yanto, 2018), Ganoderma lucidum (Agrawal et al, 2018), Pleurotus ostreatus (Pozdnyakova et al, 2018), Leucoagaricus gongylophorus (Ike et al, 2019), and Armillaria sp. FO22 (Hadibarata and Kristanti, 2012) have been reported for the remediation of PAHs contaminated soil, however as compared with bacteria, fungi is a promising alternative for the remediation of PAHs.…”

Soil characteristics constrain the response of bacterial and fungal communities and hydrocarbon degradation genes to phenanthrene soil contamination and phytoremediation with poplars

“…It is formed by three fused rings and it is relatively easy to degrade by a wide range of microorganisms through aerobic or anaerobic metabolic pathways (Cerniglia 1993). Most of microbial degraders are bacteria or archaea, although PHE degradation has been described among fungi (Haritash et Kaushik 2009; Agrawal, Verma et Shahi 2018). Typically, under aerobic conditions the initial step of the bacterial catabolic pathways starts with the incorporation of molecular oxygen into the aromatic nucleus by a ring hydroxylating dioxygenase (RHD) enzyme system to yield cis dihydrodiol.…”