Mycoplasmas Isolated from the Respiratory Tract of Cattle and Goats in Tanzania

Kusiluka, L.J.M.; Ojeniyi, B.; Friis, Ν. F.; Kazwala, R. R.; Kokotovic, Branko

doi:10.1186/bf03549638

Cited by 17 publications

(2 citation statements)

References 29 publications

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“…capripneumoniae (Mccp), Mmc, and Movi. [1][2][3]. Among these, Mccp mainly infect ungulates including sheep, goats, and wild animals [4][5][6], and it is associated with 60% mortality and 90% morbidity in goats [7].…”

Section: Introductionmentioning

confidence: 99%

Characterization and epidemiologic analysis of mycoplasmal pneumonia of sheep in Qinghai Province

Yang,

Chen,

Rahman

et al. 2024

PLoS ONE

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Mycoplasmal pneumonia in sheep and goats usually result covert but huge economic losses in the sheep and goat industry. The disease is prevalent in various countries in Africa and Asia. Clinical manifestations in affected animals include anorexia, fever, and respiratory symptoms such as dyspnea, polypnea, cough, and nasal discharge. Due to similarities with other respiratory infections, accurate diagnosis can be challenging, and isolating the causative organism is often problematic. However, the utilization of molecular techniques, such as PCR, allows for rapid and specific identification of pathogens. Thus, a goat infection model with Mycoplasma was established and the pathogen was tested using PCR. The results indicated that this approach could be effectively utilized for the rapid detection of mycoplasma in clinical settings. Additionally, the prevalence of contagious pleuropneumonia of sheep in Qinghai Province was further investigated through PCR analysis. A total of 340 nasal swabs were collected from 17 sheep farms in Qinghai province. Among these samples, 84 tested positive for Mycoplasma mycoides subsp. capri (Mmc) and 148 tested positive for Mycoplasma ovipneumoniae (Movi), resulting in positive rates of 24.71% and 43.53% respectively. Furthermore, our investigation revealed positive PCR results for nasal swabs, trachea, and lung samples obtained from sheep exhibiting symptoms suggestive of mycoplasma infection. Moreover, three distinct strains were isolated from these positive samples. Additionally, the inflammatory cytokines of peripheral blood mononuclear cells (PBMCs) were assessed using RT-PCR. The findings demonstrated a high susceptibility of sheep to Movi in Qinghai province, with infected sheep displaying an inflammatory response. Consequently, the outcomes of this study will furnish valuable epidemiological insights for the effective prevention and control of this disease within Qinghai Province.

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Section: Introductionmentioning

confidence: 99%

Characterization and epidemiologic analysis of mycoplasmal pneumonia of sheep in Qinghai Province

Yang,

Chen,

Rahman

et al. 2024

PLoS ONE

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“…Similarly, in Ethiopia prevalence of CCPP in female animals (6.66%) was found lowered compared to bucks 24.08%(Regassa et al, 2010). Various other researchers from Ethiopia and Tanzania reported that sex does not play signi cant role in epidemiology of CCPP(Kusiluka et al, 2000;Hadush et al, 2009;Mekuria et al, 2010; Yousuf et al, 2012…”

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confidence: 97%

Determination of Anti-Mycoplasma Capricolum Subsp. Capripneumoniae Antibodies For The Sero-Epidemiology of Contagious Caprine Pleuropneumonia

Ahmad¹,

Khan²,

Sadique³

et al. 2022

Preprint

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Contagious caprine pleuro-pneumonia (CCPP) is fatal disease of goats and causes huge economic losses due to high morbidity and mortality. CCPP is enlisted as notifiable animal disease by OIE. The causative agent of CCPP is Mycoplasma capricolum subsp. capripneumoniae (Mccp). The present study was aimed to investigate the seroprevalence of CCPP in northern Pakistan. The study areas were divided into three zones; northern zone, central zone and tribal zone. A total of 1300 serum samples were collected during November 2017 – April 2019 from goats of different age and sex and were analysed by monoclonal antibody based cELISA. The analyses revealed 227 (17.5%) samples positive for anti-Mccp antibodies. The zone wise distribution of CCPP in goats was significantly different (P˂0.05), indicated by positive sera for Mccp of 23% animals from northern zone followed by 15% and 13% animals from tribal and central zones, respectively. The analysis of data showed non-significant values in the seroprevalence among bucks and doe(s), indicated by anti-Mccp sera from 16.6% bucks and 18.3% doe (s). Moreover, among different age groups the prevalence of disease in adult goats (20%) was significantly (P˂0.05) higher than kids (10.8%). It is evident from the present study that CCPP caused by Mccp is prevalent in Pakistan and both sex of animals are equally susceptible to Mccp infection. Furthermore the disease is more prevalent in northern zone.

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Mycoplasma

Brown¹,

May²,

Bradbury³

et al. 2015

Bergey's Manual of Systematics of Archaea and Bacteria

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My.co.plas'ma. Gr. masc. n. myces a fungus; Gr. neut. n. plasma something formed or molded, a form; N.L. neut. n. Mycoplasma fungus form. Tenericutes / Mollicutes / Mycoplasmatales / Mycoplasmataceae / Mycoplasma Pleomorphic cells, 300–800 nm in diameter, varying in shape from spherical, ovoid or flask‐shaped, or twisted rods, to slender branched filaments ranging in length from 50 to 500 nm. Cells lack a cell wall and are bounded by a single plasma membrane. Gram‐stain‐negative due to the absence of a cell wall. Some have a complex internal cytoskeleton. Some have a specific tip structure that mediates attachment to host cells or other surfaces. Usually nonmotile , but gliding motility has been demonstrated in some species. Aerobic or facultatively anaerobic . Optimum growth at 37°C is common, but permissive growth temperatures range from 20 to 45°C. Chemo‐organotrophic , usually using either sugars or arginine as the major energy source. Require cholesterol or related sterols for growth. Colonies are usually less than 1 mm in diameter. The typical colony has a fried‐egg appearance . The genome size of species examined ranges from 580 kbp to about 1350 kbp . The codon UGA encodes tryptophan in all species examined. Commensals or pathogens in a wide range of vertebrate hosts. DNA G + C content ( mol %): 23–40. Type species : Mycoplasma mycoides (Borrel, Dujardin‐Beaumetz, Jeantet and Jouan 1910) Freundt 1955, 73 ( Asterococcus mycoides Borrel, Dujardin‐Beaumetz, Jeantet and Jouan 1910, 179).

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Mycoplasmas Isolated from the Respiratory Tract of Cattle and Goats in Tanzania

Cited by 17 publications

References 29 publications

Characterization and epidemiologic analysis of mycoplasmal pneumonia of sheep in Qinghai Province

Characterization and epidemiologic analysis of mycoplasmal pneumonia of sheep in Qinghai Province

Determination of Anti-Mycoplasma Capricolum Subsp. Capripneumoniae Antibodies For The Sero-Epidemiology of Contagious Caprine Pleuropneumonia

Mycoplasma

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