Mycoplasma-induced BALB/c 3T3 collagenase is a mammalian enzyme

Kluve, B; Merrick, William C.; Gershman, Howard

doi:10.1042/bj2120641

Cited by 5 publications

(1 citation statement)

References 19 publications

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“…Mycoplasma contamination is a common problem in laboratories that use continuously growing cell lines. However, the effect of mycoplasma contamination on specific differentiated cell functions vis a vis cell proliferation has been little studied (2,10). The data presented here show that mycoplasma infection of human B cell lines causes significant perturbation of immunoglobulin synthesis, processing, and expression.…”

Section: Discussionmentioning

confidence: 85%

Effects of mycoplasma contamination on immunoglobulin biosynthesis by human B lymphoblastoid cell lines

Hendershot¹,

Levitt²

1985

Infect Immun

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The synthesis and secretion of immunoglobulin M (IgM), as well as the relative ratio of membrane and secretory mu heavy chain (mu m and mu s, respectively), were evaluated in mycoplasma-contaminated B lymphoblastoid cell lines. The ratio of mu m to mu s was drastically lowered in infected cultures, and mu s chains could now combine with light chains. A 50 to 100% increase in IgM synthesis occurred in contaminated cultures, and small amounts of IgM were detectable in the culture media. These molecules possessed mu chains typical of secreted IgM. Reexpression of mu on the surface of B lymphoblastoid cells was substantially delayed in mycoplasma-contaminated cultures. Thus, mycoplasma contamination alters the synthesis and expression of a specific differentiated gene product (immunoglobulin) in B cell lines; such changes could significantly affect the interpretation of data on immunoglobulin synthesis by B cells with different phenotypes. This system may also provide a means of studying how mycoplasma infection alters specific gene expression in B cell lines.

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Section: Discussionmentioning

confidence: 85%

Effects of mycoplasma contamination on immunoglobulin biosynthesis by human B lymphoblastoid cell lines

Hendershot¹,

Levitt²

1985

Infect Immun

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Mycoplasmas in Cell Culture

Barile

Rottem²

1993

Rapid Diagnosis of Mycoplasmas

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Mycoplasma provides for the spreading of opossum kidney cells in a serum-free, defined medium

Leiderman

Dennis

1989

In Vitro Cell Dev Biol

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During the course of investigating the growth and differentiation of opossum kidney cells in serum-free medium, it was observed that a mycoplasma contamination (M. hyorhinis) contributed to the spreading of cells. Contaminated cells, seeded on collagen-coated plates, spread out and grew to confluency in Dulbecco's modified eagle's medium/Ham's F12 nutrient mixture containing insulin, transferrin, sodium selenite, and bovine serum albumin fraction V. In addition, differentiated characteristics, including parathyroid hormone-inhibitable, sodium-dependent phosphate transport, were expressed by these cells grown in this medium. After the infection was eradicated, the contamination-free cells would not spread out and proliferate in the same serum-free medium as they had done in the presence of mycoplasma. Normal cellular development, however, was attained after cells were plated in serum-free medium that included fetuin. Cells once again spread out, grew to confluency, and were able to express their differentiated characteristics.

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Mycoplasma-induced BALB/c 3T3 collagenase is a mammalian enzyme

Abstract: A collagenase previously reported to accumulate in the medium of cultures of BALB/c 3T3 cells on infection with Mycoplasma orale [Kluve, Merrick, Stanbridge & Gershman (1981)

Cited by 5 publications

References 19 publications

Effects of mycoplasma contamination on immunoglobulin biosynthesis by human B lymphoblastoid cell lines

Effects of mycoplasma contamination on immunoglobulin biosynthesis by human B lymphoblastoid cell lines

Mycoplasmas in Cell Culture

Mycoplasma provides for the spreading of opossum kidney cells in a serum-free, defined medium

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